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The MyoD family of myogenic factors is regulated by electrical activity: isolation and characterization of a mouse Myf-5 cDNA.
Buonanno A
,
Apone L
,
Morasso MI
,
Beers R
,
Brenner HR
,
Eftimie R
.
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A full-length cDNA coding for a homolog of the human Myf-5 was isolated from a BC3H-1 mouse library and characterized. The clone codes for a protein of 255 amino acids that is 89%, 88% and 68% identical to the human, bovine and Xenopus myf-5, respectively. The mouse Myf-5 cDNA (mmyf-5), as well as sequences coding for MyoD, myogenin and Mrf-4, were used to probe Northern blots to analyze the effects of innervation on the expression of the MyoD family of myogenic factors. Mouse myf-5, MyoD and myogenin mRNAs levels were found to decline in hind limb muscles of mice between embryonic day 15 (E15) and the first postnatal week, a period that coincides with innervation. In contrast, Mrf-4 transcripts increase during this period and reach steady-state levels by 1-week after birth. To distinguish if the changes in myogenic factor expression are due to a developmental program or to innervation, mRNA levels were analyzed at different times after muscle denervation. Mmyf-5 transcripts begin to accumulate 2 days postdenervation; after 1 week levels are 7-fold higher than in innervated muscle. Mrf-4, MyoD and myogenin transcripts begin to accumulate as soon as 8h after denervation, and attain levels that are 8-, 15- and 40-fold higher than found in innervated skeletal muscle, respectively. The accumulation of these three mRNAs precedes the increase of nicotinic acetylcholine receptor alpha subunit transcripts, a gene that is transcriptionally regulated by MyoD-related factors in vitro. Using extracellular electrodes to directly stimulate in situ the soleus muscle of rats, we found that 'electrical activity' per se, in absence of the nerve, represses the increases of myogenic factor mRNAs associated with denervation.
Braun,
Myf-6, a new member of the human gene family of myogenic determination factors: evidence for a gene cluster on chromosome 12.
1990, Pubmed
Braun,
Myf-6, a new member of the human gene family of myogenic determination factors: evidence for a gene cluster on chromosome 12.
1990,
Pubmed
Braun,
A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts.
1989,
Pubmed
Braun,
Transcriptional activation domain of the muscle-specific gene-regulatory protein myf5.
1990,
Pubmed
Brennan,
Myogenin resides in the nucleus and acquires high affinity for a conserved enhancer element on heterodimerization.
1990,
Pubmed
Bucher,
Expression of the troponin complex genes: transcriptional coactivation during myoblast differentiation and independent control in heart and skeletal muscles.
1988,
Pubmed
Buckingham,
Actin and myosin multigene families: their expression during the formation of skeletal muscle.
1985,
Pubmed
Buonanno,
Transcriptional regulation of nicotinic acetylcholine receptor genes during muscle development.
1986,
Pubmed
Buonanno,
A universal oligonucleotide probe for acetylcholine receptor genes. Selection and sequencing of cDNA clones for the mouse muscle beta subunit.
1986,
Pubmed
Buskin,
Identification of a myocyte nuclear factor that binds to the muscle-specific enhancer of the mouse muscle creatine kinase gene.
1989,
Pubmed
Cavener,
Comparison of the consensus sequence flanking translational start sites in Drosophila and vertebrates.
1987,
Pubmed
Clark,
A bovine homolog to the human myogenic determination factor myf-5: sequence conservation and 3' processing of transcripts.
1990,
Pubmed
Davis,
The MyoD DNA binding domain contains a recognition code for muscle-specific gene activation.
1990,
Pubmed
Davis,
Expression of a single transfected cDNA converts fibroblasts to myoblasts.
1987,
Pubmed
de la Brousse,
Localized expression of a myogenic regulatory gene, qmf1, in the somite dermatome of avian embryos.
1990,
Pubmed
Edmondson,
A gene with homology to the myc similarity region of MyoD1 is expressed during myogenesis and is sufficient to activate the muscle differentiation program.
1989,
Pubmed
Eftimie,
Myogenin and MyoD join a family of skeletal muscle genes regulated by electrical activity.
1991,
Pubmed
Emerson,
Myogenesis and developmental control genes.
1990,
Pubmed
Evans,
Muscle acetylcholine receptor biosynthesis. Regulation by transcript availability.
1987,
Pubmed
Fontaine,
Calcitonin gene-related peptide and muscle activity regulate acetylcholine receptor alpha-subunit mRNA levels by distinct intracellular pathways.
1987,
Pubmed
Goldman,
Acetylcholine receptor alpha-, beta-, gamma-, and delta-subunit mRNA levels are regulated by muscle activity.
1988,
Pubmed
Goldman,
Spatial and temporal expression of acetylcholine receptor RNAs in innervated and denervated rat soleus muscle.
1989,
Pubmed
Hopwood,
MyoD expression in the forming somites is an early response to mesoderm induction in Xenopus embryos.
1989,
Pubmed
,
Xenbase
Hopwood,
Xenopus Myf-5 marks early muscle cells and can activate muscle genes ectopically in early embryos.
1991,
Pubmed
,
Xenbase
Isenberg,
Nucleotide sequence of the mouse muscle nicotinic acetylcholine receptor alpha subunit.
1986,
Pubmed
Lassar,
MyoD is a sequence-specific DNA binding protein requiring a region of myc homology to bind to the muscle creatine kinase enhancer.
1989,
Pubmed
Lin,
An avian muscle factor related to MyoD1 activates muscle-specific promoters in nonmuscle cells of different germ-layer origin and in BrdU-treated myoblasts.
1989,
Pubmed
Lin,
Muscle-specific expression of the troponin I gene requires interactions between helix-loop-helix muscle regulatory factors and ubiquitous transcription factors.
1991,
Pubmed
Martinou,
Nerve-dependent modulation of acetylcholine receptor epsilon-subunit gene expression.
1991,
Pubmed
Merlie,
Neural regulation of gene expression by an acetylcholine receptor promoter in muscle of transgenic mice.
1989,
Pubmed
Merlie,
Concentration of acetylcholine receptor mRNA in synaptic regions of adult muscle fibres.
,
Pubmed
Miner,
Herculin, a fourth member of the MyoD family of myogenic regulatory genes.
1990,
Pubmed
Minty,
Mouse actin messenger RNAs. Construction and characterization of a recombinant plasmid molecule containing a complementary DNA transcript of mouse alpha-actin mRNA.
1981,
Pubmed
Montarras,
Developmental patterns in the expression of Myf5, MyoD, myogenin, and MRF4 during myogenesis.
1991,
Pubmed
Murray,
Cell proliferation in denervated muscle: identity and origin of dividing cells.
1982,
Pubmed
Murray,
Cell proliferation in denervated muscle: time course, distribution and relation to disuse.
1982,
Pubmed
Murre,
Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence.
1989,
Pubmed
Olson,
MyoD family: a paradigm for development?
1990,
Pubmed
Ontell,
Morphometric analysis of the developing mouse soleus muscle.
1988,
Pubmed
Ontell,
The organogenesis of murine striated muscle: a cytoarchitectural study.
1984,
Pubmed
Ott,
Early expression of the myogenic regulatory gene, myf-5, in precursor cells of skeletal muscle in the mouse embryo.
1991,
Pubmed
Piette,
Two adjacent MyoD1-binding sites regulate expression of the acetylcholine receptor alpha-subunit gene.
1990,
Pubmed
Pinney,
Myogenic lineage determination and differentiation: evidence for a regulatory gene pathway.
1988,
Pubmed
Rhodes,
Identification of MRF4: a new member of the muscle regulatory factor gene family.
1989,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Sassoon,
Expression of two myogenic regulatory factors myogenin and MyoD1 during mouse embryogenesis.
1989,
Pubmed
Sternberg,
Identification of upstream and intragenic regulatory elements that confer cell-type-restricted and differentiation-specific expression on the muscle creatine kinase gene.
1988,
Pubmed
Thayer,
Positive autoregulation of the myogenic determination gene MyoD1.
1989,
Pubmed
Tsay,
Skeletal muscle denervation activates acetylcholine receptor genes.
1989,
Pubmed
Weintraub,
The myoD gene family: nodal point during specification of the muscle cell lineage.
1991,
Pubmed
Wentworth,
Paired MyoD-binding sites regulate myosin light chain gene expression.
1991,
Pubmed
Witzemann,
Differential regulation of muscle acetylcholine receptor gamma- and epsilon-subunit mRNAs.
1987,
Pubmed
Wright,
Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD.
1989,
Pubmed
