Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Biol Cell
2004 Jun 01;156:2978-91. doi: 10.1091/mbc.e03-08-0613.
Show Gene links
Show Anatomy links
The adenomatous polyposis coli protein is required for the formation of robust spindles formed in CSF Xenopus extracts.
Dikovskaya D
,
Newton IP
,
Näthke IS
.
???displayArticle.abstract???
Mutations in the adenomatous polyposis coli (APC) protein occur early in colon cancer and correlate with chromosomal instability. Here, we show that depletion of APC from cystostatic factor (CSF) Xenopus extracts leads to a decrease in microtubule density and changes in tubulin distribution in spindles and asters formed in such extracts. Addition of full-length APC protein or a large, N-terminally truncated APC fragment to APC-depleted extracts restored normal spindle morphology and the intact microtubule-binding site of APC was necessary for this rescue. These data indicate that the APC protein plays a role in the formation of spindles that is dependent on its effect on microtubules. Spindles formed in cycled extracts were not sensitive to APC depletion. In CSF extracts, spindles predominantly formed from aster-like intermediates, whereas in cycled extracts chromatin was the major site of initial microtubule polymerization. These data suggest that APC is important for centrosomally driven spindle formation, which was confirmed by our finding that APC depletion reduced the size of asters nucleated from isolated centrosomes. We propose that lack of microtubule binding in cancer-associated mutations of APC may contribute to defects in the assembly of mitotic spindles and lead to missegregation of chromosomes.
Bienz,
Linking colorectal cancer to Wnt signaling.
2000, Pubmed
Bienz,
Linking colorectal cancer to Wnt signaling.
2000,
Pubmed
Carazo-Salas,
Long-range communication between chromatin and microtubules in Xenopus egg extracts.
2003,
Pubmed
,
Xenbase
Deka,
A domain within the tumor suppressor protein APC shows very similar biochemical properties as the microtubule-associated protein tau.
1998,
Pubmed
Desai,
The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.
1999,
Pubmed
,
Xenbase
Dikovskaya,
The adenomatous polyposis coli protein: in the limelight out at the edge.
2001,
Pubmed
Fearnhead,
The ABC of APC.
2001,
Pubmed
Fodde,
Mutations in the APC tumour suppressor gene cause chromosomal instability.
2001,
Pubmed
Green,
Chromosome instability in colorectal tumor cells is associated with defects in microtubule plus-end attachments caused by a dominant mutation in APC.
2003,
Pubmed
Heald,
Spindle assembly in Xenopus egg extracts: respective roles of centrosomes and microtubule self-organization.
1997,
Pubmed
,
Xenbase
Hyman,
Preparation of modified tubulins.
1991,
Pubmed
Hyman,
The role of nucleation in patterning microtubule networks.
1998,
Pubmed
Jimbo,
Identification of a link between the tumour suppressor APC and the kinesin superfamily.
2002,
Pubmed
,
Xenbase
Juwana,
EB/RP gene family encodes tubulin binding proteins.
1999,
Pubmed
Kaplan,
A role for the Adenomatous Polyposis Coli protein in chromosome segregation.
2001,
Pubmed
Kline-Smith,
Depletion of centromeric MCAK leads to chromosome congression and segregation defects due to improper kinetochore attachments.
2004,
Pubmed
,
Xenbase
Lee,
The roles of APC and Axin derived from experimental and theoretical analysis of the Wnt pathway.
2003,
Pubmed
,
Xenbase
Louie,
Adenomatous polyposis coli and EB1 localize in close proximity of the mother centriole and EB1 is a functional component of centrosomes.
2004,
Pubmed
Murray,
Cell cycle extracts.
1991,
Pubmed
Näthke,
The adenomatous polyposis coli tumor suppressor protein localizes to plasma membrane sites involved in active cell migration.
1996,
Pubmed
Nigg,
Mitotic kinases as regulators of cell division and its checkpoints.
2001,
Pubmed
Polakis,
The adenomatous polyposis coli (APC) tumor suppressor.
1997,
Pubmed
Polakis,
More than one way to skin a catenin.
2001,
Pubmed
Sawin,
Mitotic spindle assembly by two different pathways in vitro.
1991,
Pubmed
,
Xenbase
Scholey,
Cell division.
2003,
Pubmed
Shih,
Evidence that genetic instability occurs at an early stage of colorectal tumorigenesis.
2001,
Pubmed
Su,
APC binds to the novel protein EB1.
1995,
Pubmed
Tighe,
Aneuploid colon cancer cells have a robust spindle checkpoint.
2001,
Pubmed
Tirnauer,
EB1-microtubule interactions in Xenopus egg extracts: role of EB1 in microtubule stabilization and mechanisms of targeting to microtubules.
2002,
Pubmed
,
Xenbase
Wood,
CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment.
1997,
Pubmed
,
Xenbase
Zumbrunn,
Binding of the adenomatous polyposis coli protein to microtubules increases microtubule stability and is regulated by GSK3 beta phosphorylation.
2001,
Pubmed
