Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cell Stem Cell
2008 Jan 10;21:90-102. doi: 10.1016/j.stem.2007.10.002.
Show Gene links
Show Anatomy links
Lentiviral transduction of mammary stem cells for analysis of gene function during development and cancer.
Welm BE
,
Dijkgraaf GJ
,
Bledau AS
,
Welm AL
,
Werb Z
.
???displayArticle.abstract???
The mouse mammary gland is the only epithelial organ capable of complete regeneration upon orthotopic transplantation, making it ideally suited for in vivo gene function studies through viral-mediated gene delivery. A hurdle that has challenged the widespread adoption of this technique has been the inability to transduce mammary stem cells effectively. We have overcome this limitation by infecting total primary mammary epithelial cells in suspension with high-titer lentiviruses. Transduced cells gave rise to all major cell types of the mammary gland and were capable of clonal outgrowth and functional differentiation in serial transplants. To demonstrate that this method is a valuable alternative to developing transgenic animals, we used lentiviral-mediated Wnt-1 overexpression to replicate MMTV-Wnt-1 mammary phenotypes and used a dominant-negative Xenopus Suppressor of Hairless to reveal a requirement for Notch signaling during ductal morphogenesis. Importantly, this method is also applicable to transduction of cells from other tissues.
Batra,
Retroviral gene transfer is inhibited by chondroitin sulfate proteoglycans/glycosaminoglycans in malignant pleural effusions.
1997, Pubmed
Batra,
Retroviral gene transfer is inhibited by chondroitin sulfate proteoglycans/glycosaminoglycans in malignant pleural effusions.
1997,
Pubmed
Borok,
Vesicular stomatitis virus G-pseudotyped lentivirus vectors mediate efficient apical transduction of polarized quiescent primary alveolar epithelial cells.
2001,
Pubmed
Bray,
Notch signalling: a simple pathway becomes complex.
2006,
Pubmed
Buono,
The canonical Notch/RBP-J signaling pathway controls the balance of cell lineages in mammary epithelium during pregnancy.
2006,
Pubmed
Burns,
Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells.
1993,
Pubmed
Campbell,
A monomeric red fluorescent protein.
2002,
Pubmed
Clarkson,
IkB kinase alpha: a link in the chain of the mammary cycle.
2002,
Pubmed
Coffin,
NULL
1997,
Pubmed
Cormack,
FACS-optimized mutants of the green fluorescent protein (GFP).
1996,
Pubmed
Daniel,
The in vivo life span of normal and preneoplastic mouse mammary glands: a serial transplantation study.
1968,
Pubmed
DEOME,
Development of mammary tumors from hyperplastic alveolar nodules transplanted into gland-free mammary fat pads of female C3H mice.
1959,
Pubmed
Du,
Introduction of oncogenes into mammary glands in vivo with an avian retroviral vector initiates and promotes carcinogenesis in mouse models.
2006,
Pubmed
Duncan,
Integration of Notch and Wnt signaling in hematopoietic stem cell maintenance.
2005,
Pubmed
Edwards,
Genetic manipulation of mammary epithelium by transplantation.
1996,
Pubmed
Edwards,
Hyperplasia of mouse mammary epithelium induced by expression of the Wnt-1 (int-1) oncogene in reconstituted mammary gland.
1992,
Pubmed
Hinck,
Key stages in mammary gland development: the mammary end bud as a motile organ.
2005,
Pubmed
Johnson,
Pseudotyped human lentiviral vector-mediated gene transfer to airway epithelia in vivo.
2000,
Pubmed
Kanda,
Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells.
1998,
Pubmed
Kim,
Use of the human elongation factor 1 alpha promoter as a versatile and efficient expression system.
1990,
Pubmed
Kordon,
An entire functional mammary gland may comprise the progeny from a single cell.
1998,
Pubmed
Kuperwasser,
Reconstruction of functionally normal and malignant human breast tissues in mice.
2004,
Pubmed
Matz,
Fluorescent proteins from nonbioluminescent Anthozoa species.
1999,
Pubmed
,
Xenbase
Pickett,
Occluding junctions and cell behavior in primary cultures of normal and neoplastic mammary gland cells.
1975,
Pubmed
Richert,
An atlas of mouse mammary gland development.
2000,
Pubmed
Shackleton,
Generation of a functional mammary gland from a single stem cell.
2006,
Pubmed
Shin,
Tight junctions and cell polarity.
2006,
Pubmed
Smith,
Long-term in vivo expression of genes introduced by retrovirus-mediated transfer into mammary epithelial cells.
1991,
Pubmed
Smith,
Experimental mammary epithelial morphogenesis in an in vivo model: evidence for distinct cellular progenitors of the ductal and lobular phenotype.
1996,
Pubmed
Smith,
Mammary stem cell repertoire: new insights in aging epithelial populations.
2002,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Sternlicht,
Hormonal and local control of mammary branching morphogenesis.
2006,
Pubmed
Stingl,
Purification and unique properties of mammary epithelial stem cells.
2006,
Pubmed
Streuli,
Adhesion-mediated signaling in the regulation of mammary epithelial cell survival.
1999,
Pubmed
Tsukamoto,
Expression of the int-1 gene in transgenic mice is associated with mammary gland hyperplasia and adenocarcinomas in male and female mice.
1988,
Pubmed
Van Parijs,
Uncoupling IL-2 signals that regulate T cell proliferation, survival, and Fas-mediated activation-induced cell death.
1999,
Pubmed
Ventura,
Cre-lox-regulated conditional RNA interference from transgenes.
2004,
Pubmed
Wagner,
Cre-mediated gene deletion in the mammary gland.
1997,
Pubmed
Welm,
MET and MYC cooperate in mammary tumorigenesis.
2005,
Pubmed
Wettstein,
The Xenopus homolog of Drosophila Suppressor of Hairless mediates Notch signaling during primary neurogenesis.
1997,
Pubmed
,
Xenbase
Wiseman,
Stromal effects on mammary gland development and breast cancer.
2002,
Pubmed
Young,
The influence of host and tissue age on life span and growth rate of serially transplanted mouse mammary gland.
1971,
Pubmed
