Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Neurophysiol
2008 Oct 01;1004:2287-99. doi: 10.1152/jn.90707.2008.
Show Gene links
Show Anatomy links
Molecular identity and functional properties of a novel T-type Ca2+ channel cloned from the sensory epithelia of the mouse inner ear.
Nie L
,
Zhu J
,
Gratton MA
,
Liao A
,
Mu KJ
,
Nonner W
,
Richardson GP
,
Yamoah EN
.
???displayArticle.abstract???
The molecular identity of non-Cav1.3 channels in auditory and vestibular hair cells has remained obscure, yet the evidence in support of their roles to promote diverse Ca2+-dependent functions is indisputable. Recently, a transient Cav3.1 current that serves as a functional signature for the development and regeneration of hair cells has been identified in the chicken basilar papilla. The Cav3.1 current promotes spontaneous activity of the developing hair cell, which may be essential for synapse formation. Here, we have isolated and sequenced the full-length complementary DNA of a distinct isoform of Cav3.1 in the mouse inner ear. The channel is derived from alternative splicing of exon14, exon25A, exon34, and exon35. Functional expression of the channel in Xenopus oocytes yielded Ca2+ currents, which have a permeation phenotype consistent with T-type channels. However, unlike most multiion channels, the T-type channel does not exhibit the anomalous mole fraction effect, possibly reflecting comparable permeation properties of divalent cations. The Cav3.1 channel was expressed in sensory and nonsensory epithelia of the inner ear. Moreover, there are profound changes in the expression levels during development. The differential expression of the channel during development and the pharmacology of the inner earCav3.1 channel may have contributed to the difficulties associated with identification of the non-Cav1.3 currents.
Art,
Variation of membrane properties in hair cells isolated from the turtle cochlea.
1987, Pubmed
Art,
Variation of membrane properties in hair cells isolated from the turtle cochlea.
1987,
Pubmed
Balkowiec,
Cellular mechanisms regulating activity-dependent release of native brain-derived neurotrophic factor from hippocampal neurons.
2002,
Pubmed
Bao,
Voltage-gated calcium channel currents in type I and type II hair cells isolated from the rat crista.
2003,
Pubmed
Barish,
Development of intracellular calcium responses to depolarization and to kainate and N-methyl-D-aspartate in cultured mouse hippocampal neurons.
1991,
Pubmed
Bertolesi,
Regulation of alpha1G T-type calcium channel gene (CACNA1G) expression during neuronal differentiation.
2003,
Pubmed
Bringmann,
Developmental regulation of calcium channel-mediated currents in retinal glial (Müller) cells.
2000,
Pubmed
Chameau,
Development of multiple calcium channel types in cultured mouse hippocampal neurons.
1999,
Pubmed
Chemin,
Neuronal T-type alpha 1H calcium channels induce neuritogenesis and expression of high-voltage-activated calcium channels in the NG108-15 cell line.
2002,
Pubmed
Chemin,
Specific contribution of human T-type calcium channel isotypes (alpha(1G), alpha(1H) and alpha(1I)) to neuronal excitability.
2002,
Pubmed
Chemin,
Alternatively spliced alpha(1G) (Ca(V)3.1) intracellular loops promote specific T-type Ca(2+) channel gating properties.
2001,
Pubmed
Church,
Single L-type calcium channel conductance with physiological levels of calcium in chick ciliary ganglion neurons.
1996,
Pubmed
Desmadryl,
Developmental regulation of T-, N- and L-type calcium currents in mouse embryonic sensory neurones.
1998,
Pubmed
Dolphin,
The effect of alpha2-delta and other accessory subunits on expression and properties of the calcium channel alpha1G.
1999,
Pubmed
,
Xenbase
Dou,
Null mutation of alpha1D Ca2+ channel gene results in deafness but no vestibular defect in mice.
2004,
Pubmed
Eatock,
Functional development of hair cells.
2003,
Pubmed
Emerick,
Profiling the array of Ca(v)3.1 variants from the human T-type calcium channel gene CACNA1G: alternative structures, developmental expression, and biophysical variations.
2006,
Pubmed
Fariñas,
Spatial shaping of cochlear innervation by temporally regulated neurotrophin expression.
2001,
Pubmed
Fuchs,
Calcium currents in hair cells isolated from the cochlea of the chick.
1990,
Pubmed
Hagiwara,
Contribution of two types of calcium currents to the pacemaker potentials of rabbit sino-atrial node cells.
1988,
Pubmed
Hagiwara,
Surface density of calcium ions and calcium spikes in the barnacle muscle fiber membrane.
1967,
Pubmed
Hagiwara,
Studies of calcium channels in rat clonal pituitary cells with patch electrode voltage clamp.
1982,
Pubmed
Holley,
Keynote review: The auditory system, hearing loss and potential targets for drug development.
2005,
Pubmed
Hudspeth,
Kinetic analysis of voltage- and ion-dependent conductances in saccular hair cells of the bull-frog, Rana catesbeiana.
1988,
Pubmed
Huguenard,
Transient Ca2+ currents in neurons isolated from rat lateral habenula.
1993,
Pubmed
Huguenard,
Low-threshold calcium currents in central nervous system neurons.
1996,
Pubmed
Inagaki,
The CaV3.1 T-type Ca2+channel contributes to voltage-dependent calcium currents in rat outer hair cells.
2008,
Pubmed
Kollmar,
Predominance of the alpha1D subunit in L-type voltage-gated Ca2+ channels of hair cells in the chicken's cochlea.
1997,
Pubmed
Lacerda,
T-type and N-type calcium channels of Xenopus oocytes: evidence for specific interactions with beta subunits.
1994,
Pubmed
,
Xenbase
Lee,
Actions of mibefradil, efonidipine and nifedipine block of recombinant T- and L-type Ca channels with distinct inhibitory mechanisms.
2006,
Pubmed
Leuranguer,
Antisense depletion of beta-subunits fails to affect T-type calcium channels properties in a neuroblastoma cell line.
1998,
Pubmed
Levic,
Development and regeneration of hair cells share common functional features.
2007,
Pubmed
Llinás,
Properties and distribution of ionic conductances generating electroresponsiveness of mammalian inferior olivary neurones in vitro.
1981,
Pubmed
Martini,
Calcium currents in hair cells isolated from semicircular canals of the frog.
2000,
Pubmed
Michna,
Cav1.3 (alpha1D) Ca2+ currents in neonatal outer hair cells of mice.
2003,
Pubmed
Mittman,
Structure and alternative splicing of the gene encoding alpha1G, a human brain T calcium channel alpha1 subunit.
1999,
Pubmed
Monteil,
Molecular and functional properties of the human alpha(1G) subunit that forms T-type calcium channels.
2000,
Pubmed
Murbartián,
Functional impact of alternative splicing of human T-type Cav3.3 calcium channels.
2004,
Pubmed
Nahm,
Differential expression of T-type calcium channels in P/Q-type calcium channel mutant mice with ataxia and absence epilepsy.
2005,
Pubmed
Nie,
Cloning and expression of a small-conductance Ca(2+)-activated K+ channel from the mouse cochlea: coexpression with alpha9/alpha10 acetylcholine receptors.
2004,
Pubmed
,
Xenbase
Perez-Reyes,
Molecular characterization of a novel family of low voltage-activated, T-type, calcium channels.
1998,
Pubmed
,
Xenbase
Perez-Reyes,
Molecular physiology of low-voltage-activated t-type calcium channels.
2003,
Pubmed
Pirchio,
Postnatal development of the T calcium current in cat thalamocortical cells.
1990,
Pubmed
Platzer,
Congenital deafness and sinoatrial node dysfunction in mice lacking class D L-type Ca2+ channels.
2000,
Pubmed
Rennie,
Ionic currents in isolated vestibular hair cells from the guinea-pig crista ampullaris.
1991,
Pubmed
Rodman,
The low-voltage-activated calcium channel CAV3.1 controls proliferation of human pulmonary artery myocytes.
2005,
Pubmed
Rodman,
Low-voltage-activated (T-type) calcium channels control proliferation of human pulmonary artery myocytes.
2005,
Pubmed
Rodriguez-Contreras,
Ca2+ transport properties and determinants of anomalous mole fraction effects of single voltage-gated Ca2+ channels in hair cells from bullfrog saccule.
2002,
Pubmed
Rodriguez-Contreras,
Direct measurement of single-channel Ca(2+) currents in bullfrog hair cells reveals two distinct channel subtypes.
2001,
Pubmed
Rodríguez-Contreras,
Effects of permeant ion concentrations on the gating of L-type Ca2+ channels in hair cells.
2003,
Pubmed
Schnee,
Biophysical and pharmacological characterization of voltage-gated calcium currents in turtle auditory hair cells.
2003,
Pubmed
Strobeck,
Morphological transformation induced by activation of the mitogen-activated protein kinase pathway requires suppression of the T-type Ca2+ channel.
1999,
Pubmed
Su,
Two types of calcium channels in bullfrog saccular hair cells.
1995,
Pubmed
Talley,
Differential distribution of three members of a gene family encoding low voltage-activated (T-type) calcium channels.
1999,
Pubmed
Wang,
A low voltage-activated Ca2+ current mediates cytokine-induced pancreatic beta-cell death.
1999,
Pubmed
Yaari,
Development of two types of calcium channels in cultured mammalian hippocampal neurons.
1987,
Pubmed
Yunker,
Low-voltage-activated ("T-Type") calcium channels in review.
2003,
Pubmed
Zhou,
Surface charge and calcium channel saturation in bullfrog sympathetic neurons.
1995,
Pubmed
Zidanic,
Kinetic analysis of barium currents in chick cochlear hair cells.
1995,
Pubmed
