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Fish Shellfish Immunol
2009 Sep 01;273:397-406. doi: 10.1016/j.fsi.2009.05.020.
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Molecular characterization of the alpha subunit of complement component C8 (GcC8alpha) in the nurse shark (Ginglymostoma cirratum).
Aybar L
,
Shin DH
,
Smith SL
.
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Target cell lysis by complement is achieved by the assembly and insertion of the membrane attack complex (MAC) composed of glycoproteins C5b through C9. The lytic activity of shark complement involves functional analogues of mammalian C8 and C9. Mammalian C8 is composed of alpha, beta, and gamma subunits. The subunit structure of shark C8 is not known. This report describes a 2341 nucleotide sequence that translates into a polypeptide of 589 amino acid residues, orthologue to mammalian C8alpha and has the same modular architecture with conserved cysteines forming the peptide bond backbone. The C8gamma-binding cysteine is conserved in the perforin-like domain. Hydrophobicity profile indicates the presence of hydrophobic residues essential for membrane insertion. It shares 41.1% and 47.4% identity with human and Xenopus C8alpha respectively. Southern blot analysis showed GcC8alpha exists as a single copy gene expressed in most tissues except the spleen with the liver being the main site of synthesis. Phylogenetic analysis places it in a clade with C8alpha orthologs and as a sister taxa to the Xenopus.
Altschul,
Basic local alignment search tool.
1990, Pubmed
Altschul,
Basic local alignment search tool.
1990,
Pubmed
Azumi,
Genomic analysis of immunity in a Urochordate and the emergence of the vertebrate immune system: "waiting for Godot".
2003,
Pubmed
Brickner,
Function of subunits within the eighth component of human complement: selective removal of the gamma chain reveals it has no direct role in cytolysis.
1984,
Pubmed
Brickner,
Functional domains of the alpha subunit of the eighth component of human complement: identification and characterization of a distinct binding site for the gamma chain.
1985,
Pubmed
Chondrou,
cDNA cloning and phylogenetic analysis of the sixth complement component in rainbow trout.
2006,
Pubmed
Chondrou,
Three isoforms of complement properdin factor P in trout: cloning, expression, gene organization and constrained modeling.
2008,
Pubmed
,
Xenbase
Dishaw,
Characterization of a C3-like cDNA in a coral: phylogenetic implications.
2005,
Pubmed
Esser,
The membrane attack complex of complement. Assembly, structure and cytotoxic activity.
1994,
Pubmed
Frohman,
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
1988,
Pubmed
Fujii,
Immunity in lamprey. III. Occurrence of the complement-like activity.
1981,
Pubmed
Gongora,
Independent duplications of Bf and C3 complement genes in the zebrafish.
1998,
Pubmed
Graham,
Molecular and expression analysis of complement component C5 in the nurse shark (Ginglymostoma cirratum) and its predicted functional role.
2009,
Pubmed
Hadders,
Structure of C8alpha-MACPF reveals mechanism of membrane attack in complement immune defense.
2007,
Pubmed
Haefliger,
Structural and functional characterization of complement C8 gamma, a member of the lipocalin protein family.
1991,
Pubmed
Hobart,
Structure of the human C7 gene and comparison with the C6, C8A, C8B, and C9 genes.
1995,
Pubmed
Hofsteenge,
The four terminal components of the complement system are C-mannosylated on multiple tryptophan residues.
1999,
Pubmed
Humphrey,
The lesions in cell membranes caused by complement.
1969,
Pubmed
Jensen,
The complement system of the nurse shark: hemolytic and comparative characteristics.
1981,
Pubmed
Katagiri,
Molecular analysis of complement component C8beta and C9 cDNAs of Japanese flounder, Paralichthys olivaceus.
1999,
Pubmed
Kato,
The complement component C5 of the common carp (Cyprinus carpio): cDNA cloning of two distinct isotypes that differ in a functional site.
2003,
Pubmed
Kauffman,
Alignment of amino acid and DNA sequences of human proline-rich proteins.
1993,
Pubmed
Kazantzi,
Molecular cloning of the beta subunit of complement component eight of rainbow trout.
2003,
Pubmed
Kuroda,
Molecular cloning and linkage analysis of the Japanese medaka fish complement Bf/C2 gene.
1996,
Pubmed
,
Xenbase
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Legler,
Comparative immunology: hemolytic complement in elasmobranchs.
1967,
Pubmed
Marshall,
The nature and metabolism of the carbohydrate-peptide linkages of glycoproteins.
1974,
Pubmed
Mondragón-Palomino,
Phylogenetic analysis of the homologous proteins of the terminal complement complex supports the emergence of C6 and C7 followed by C8 and C9.
1999,
Pubmed
Müller-Eberhard,
The membrane attack complex of complement.
1986,
Pubmed
Nakao,
Two diverged complement factor B/C2-like cDNA sequences from a teleost, the common carp (Cyprinus carpio).
1998,
Pubmed
Nakao,
Diversity of complement factor B/C2 in the common carp (Cyprinus carpio): three isotypes of B/C2-A expressed in different tissues.
2002,
Pubmed
Ng,
The eighth component of human complement: evidence that it is an oligomeric serum protein assembled from products of three different genes.
1987,
Pubmed
Nonaka,
Complement system of bony and cartilaginous fish.
2000,
Pubmed
Papanastasiou,
Gene duplication of the seventh component of complement in rainbow trout.
2005,
Pubmed
Papanastasiou,
Cloning and phylogenetic analysis of the alpha subunit of the eighth complement component (C8) in rainbow trout.
2006,
Pubmed
Papanastasiou,
Cloning of a CD59-like gene in rainbow trout. Expression and phylogenetic analysis of two isoforms.
2007,
Pubmed
Papanastasiou,
The gamma subunit of the eighth complement component (C8) in rainbow trout.
2006,
Pubmed
Peitsch,
Localization and molecular modelling of the membrane-inserted domain of the ninth component of human complement and perforin.
1990,
Pubmed
Plumb,
An indel within the C8 alpha subunit of human complement C8 mediates intracellular binding of C8 gamma and formation of C8 alpha-gamma.
2000,
Pubmed
Podack,
A central role of perforin in cytolysis?
1991,
Pubmed
Podack,
Membrane attack by complement.
1984,
Pubmed
Saitou,
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
1987,
Pubmed
Shin,
Characterization of shark complement factor I gene(s): genomic analysis of a novel shark-specific sequence.
2009,
Pubmed
Shin,
Molecular cloning, structural analysis and expression of complement component Bf/C2 genes in the nurse shark, Ginglymostoma cirratum.
2007,
Pubmed
Slade,
Functional studies of the MACPF domain of human complement protein C8alpha reveal sites for simultaneous binding of C8beta, C8gamma, and C9.
2006,
Pubmed
Smith,
Shark complement: an assessment.
1998,
Pubmed
Smith,
The second component (C2n) of the nurse shark complement system: purification, physico-chemical characterization and functional comparison with guinea pig C4.
1986,
Pubmed
Smith,
Complement systems in invertebrates. The ancient alternative and lectin pathways.
1999,
Pubmed
Sodetz,
Structure and function of C8 in the membrane attack sequence of complement.
1989,
Pubmed
Steckel,
The eighth component of human complement. Purification and physicochemical characterization of its unusual subunit structure.
1980,
Pubmed
Sunyer,
Cloning, structure, and function of two rainbow trout Bf molecules.
1998,
Pubmed
Sunyer,
Multiple forms of complement C3 in trout that differ in binding to complement activators.
1996,
Pubmed
Sunyer,
Structural C3 diversity in fish: characterization of five forms of C3 in the diploid fish Sparus aurata.
1997,
Pubmed
Suzuki,
C6-like and C3-like molecules from the cephalochordate, amphioxus, suggest a cytolytic complement system in invertebrates.
2002,
Pubmed
Thompson,
Reactive lysis: the complement-mediated lysis of unsensitized cells. I. The characterization of the indicator factor and its identification as C7.
1970,
Pubmed
Thompson,
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
1997,
Pubmed
Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed
Uemura,
Purification and characterization of the eighth and ninth components of carp complement.
1996,
Pubmed
Wakoh,
Identification of transcripts expressed preferentially in hemocytes of Ciona intestinalis that can be used as molecular markers.
2004,
Pubmed
Walport,
Complement. Second of two parts.
2001,
Pubmed
Walport,
Complement. First of two parts.
2001,
Pubmed
Wells,
Glycosylation of nucleocytoplasmic proteins: signal transduction and O-GlcNAc.
2001,
Pubmed
Young,
The pore-forming protein (perforin) of cytolytic T lymphocytes is immunologically related to the components of membrane attack complex of complement through cysteine-rich domains.
1986,
Pubmed
Zarkadis,
Molecular cloning of the seventh component of complement in rainbow trout.
2005,
Pubmed
