Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Comp Biochem Physiol B Biochem Mol Biol
2010 Mar 01;1553:256-65. doi: 10.1016/j.cbpb.2009.11.008.
Show Gene links
Show Anatomy links
Vitellogenesis in Bufo arenarum: identification, characterization and immunolocalization of high molecular mass lipovitellin during oogenesis.
O'Brien ED, Salicioni AM, Cabada MO, Arranz SE.
???displayArticle.abstract???
Vitellogenin (Vtg), a large lipoglycophosphoprotein, is the most important precursor of the yolk proteins, and the major source of nutrients for the developing embryo in oviparous species. After its uptake by the oocytes, Vtg is converted into lipovitellins (high and light) and phosvitin, which are deposited into crystalline yolk platelets. We describe here the presence of two high molecular mass lipovitellin isoforms in Bufo arenarum mature oocytes with masses of 113 and 100 kDa, respectively. The amino acid sequence analysis of p113 and p100 peptides showed a high sequence homology between both polypeptides and the complete reported sequences of Xenopus laevis vitellogenin. Using specific antibodies, we determined that the Vtg uptake begins early during oogenesis, at the previtellogenic stage, and continues until oocytes have reached their mature status. In addition, we found that large endocytic vesicles mediate Vtg uptake in stage I oocytes, and that the size of the endocytic vesicles declines with oogenesis progression. In terms of the Vtg protein trafficking, we detected the Vtg precursor (190 kDa) in the liver of estradiol-injected females. Finally, we propose a subclassification of B. arenarum stage II oocytes into three physiologically and morphologically distinct periods (early, mid and late).
Armas,
Primary structure and developmental expression of Bufo arenarum cellular nucleic acid-binding protein: changes in subcellular localization during early embryogenesis.
2001, Pubmed
Armas,
Primary structure and developmental expression of Bufo arenarum cellular nucleic acid-binding protein: changes in subcellular localization during early embryogenesis.
2001,
Pubmed Arranz,
Bufo arenarum egg jelly coat: purification and characterization of two highly glycosylated proteins.
1997,
Pubmed Barisone,
Vitelline envelope of Bufo arenarum: biochemical and biological characterization.
2002,
Pubmed
,
Xenbase Benbow,
Cytoplasmic control of nuclear DNA synthesis during early development of Xenopus laevis: a cell-free assay.
1975,
Pubmed
,
Xenbase Cabada,
Vitelline envelope formation during oogenesis in Bufo arenarum.
1996,
Pubmed Callen,
Development of the mitochondrial mass and accumulation of mtDNA in previtellogenic stages of Xenopus laevis oocytes.
1980,
Pubmed
,
Xenbase Chenna,
Multiple sequence alignment with the Clustal series of programs.
2003,
Pubmed Danilchik,
Differentiation of the animal-vegetal axis in Xenopus laevis oocytes. I. Polarized intracellular translocation of platelets establishes the yolk gradient.
1987,
Pubmed
,
Xenbase Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase Finn,
Vertebrate yolk complexes and the functional implications of phosvitins and other subdomains in vitellogenins.
2007,
Pubmed Gerber-Huber,
Precursor-product relationship between vitellogenin and the yolk proteins as derived from the complete sequence of a Xenopus vitellogenin gene.
1987,
Pubmed
,
Xenbase Gradilone,
Detection of highly glycosylated proteins in polyacrylamide gels.
1998,
Pubmed Kristoffersen,
Genomic and proteomic analyses reveal non-neofunctionalized vitellogenins in a basal clupeocephalan, the Atlantic herring, and point to the origin of maturational yolk proteolysis in marine teleosts.
2009,
Pubmed Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Molla,
Xenopus lipovitellin, a new target protein for calmodulin.
1983,
Pubmed
,
Xenbase Opresko,
Differential postendocytotic compartmentation in Xenopus oocytes is mediated by a specifically bound ligand.
1980,
Pubmed
,
Xenbase Opresko,
Receptor-mediated endocytosis in Xenopus oocytes. I. Characterization of the vitellogenin receptor system.
1987,
Pubmed
,
Xenbase Perazzolo,
Expression and localization of messenger ribonucleic acid for the vitellogenin receptor in ovarian follicles throughout oogenesis in the rainbow trout, Oncorhynchus mykiss.
1999,
Pubmed Plaxton,
Molecular and immunological characterization of plastid and cytosolic pyruvate kinase isozymes from castor-oil-plant endosperm and leaf.
1989,
Pubmed Redshaw,
The crystalline yolk-platelet proteins and their soluble plasma precursor in an amphibian, Xenopus laevis.
1971,
Pubmed
,
Xenbase Romano,
Vertebrate yolk proteins: a review.
2004,
Pubmed Schmid,
Evolutionary diversity of reverse (R) fluorescent chromosome bands in vertebrates.
1988,
Pubmed Stifani,
Regulation of oogenesis: the piscine receptor for vitellogenin.
1990,
Pubmed
,
Xenbase Stifani,
Solubilization and characterization of the chicken oocyte vitellogenin receptor.
1988,
Pubmed Tata,
Vitellogenesis: a versatile model for hormonal regulation of gene expression.
1979,
Pubmed
,
Xenbase Thompson,
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
1997,
Pubmed Valz-Gianinet,
Glycoproteins from Bufo arenarum vitelline envelope with fertility-impairing effect on homologous spermatozoa.
1991,
Pubmed Villecco,
Comparative study of vitellogenesis in the anuran amphibians Ceratophrys cranwelli (Leptodactilidae) and Bufo arenarum (Bufonidae).
1999,
Pubmed Wahli,
Vitellogenin genes A1 and B1 are linked in the Xenopus laevis genome.
1982,
Pubmed
,
Xenbase Wall,
The intracellular fate of vitellogenin in Xenopus oocytes is determined by its extracellular concentration during endocytosis.
1987,
Pubmed
,
Xenbase Wallace,
Studies on amphibian yolk. IX. Xenopus vitellogenin.
1970,
Pubmed
,
Xenbase Wallace,
Oogenesis in Fundulus heteroclitus. II. The transition from vitellogenesis into maturation.
1980,
Pubmed Wallace,
Protein incorporation by isolated amphibian oocytes. II. A survey of inhibitors.
1972,
Pubmed
,
Xenbase Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase Wallace,
Placement of small lipovitellin subunits within the vitellogenin precursor in Xenopus laevis.
1990,
Pubmed
,
Xenbase Wallace,
Vitellogenesis and oocyte growth in nonmammalian vertebrates.
1985,
Pubmed Wallace,
The induced synthesis and transport of yolk proteins and their accumulation by the oocyte in Xenopus laevis.
1968,
Pubmed
,
Xenbase Wang,
Improved procedures for N-terminal sulfonation of peptides for matrix-assisted laser desorption/ionization post-source decay peptide sequencing.
2004,
Pubmed Ward,
The origin of protein and fatty yolk in Rana pipiens. IV. Secondary vesicular yolk formation in frog oocytes.
1978,
Pubmed Wiley,
The structure of vitellogenin. Multiple vitellogenins in Xenopus laevis give rise to multiple forms of the yolk proteins.
1981,
Pubmed
,
Xenbase Winter,
Yolk proteins and their plasmatic precursor in the tetraploid Odontophrynus americanus (Amphibia, Anura).
1985,
Pubmed Yoshitome,
Mr 25 000 protein, a substrate for protein serine/threonine kinases, is identified as a part of Xenopus laevis vitellogenin B1.
2003,
Pubmed
,
Xenbase
