Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Curr Biol
2013 Jun 17;2312:1046-56. doi: 10.1016/j.cub.2013.04.057.
Show Gene links
Show Anatomy links
Dynamic localization of G-actin during membrane protrusion in neuronal motility.
Vitriol EA
,
Shim S
,
Wise AL
,
Velayutham RP
,
Zheng JQ
.
???displayArticle.abstract???
Actin-based cell motility is fundamental for development, function, and malignant events in eukaryotic organisms. During neural development, axonal growth cones depend on rapid assembly and disassembly of actin filaments (F-actin) for their guided extension to specific targets for wiring. Monomeric globular actin (G-actin) is the building block for F-actin but is not considered to play a direct role in spatiotemporal control of actin dynamics in cell motility. Here we report that a pool of G-actin dynamically localizes to the leading edge of growth cones and neuroblastoma cells to spatially elevate the G-/F-actin ratio that drives membrane protrusion and cell movement. Loss of G-actin localization leads to the cessation and retraction of membrane protrusions. Moreover, G-actin localization occurs asymmetrically in growth cones during attractive turning. Finally, we identify the actin monomer-binding proteins profilin and thymosin β4 as key molecules that localize actin monomers to the leading edge of lamellipodia for their motility. Our results suggest that dynamic localization of G-actin provides a novel mechanism to regulate the spatiotemporal actin dynamics underlying membrane protrusion in cell locomotion and growth cone chemotaxis.
Bubb,
Jasplakinolide, a cytotoxic natural product, induces actin polymerization and competitively inhibits the binding of phalloidin to F-actin.
1994, Pubmed
Bubb,
Jasplakinolide, a cytotoxic natural product, induces actin polymerization and competitively inhibits the binding of phalloidin to F-actin.
1994,
Pubmed
Cao,
Localization and dynamics of nonfilamentous actin in cultured cells.
1993,
Pubmed
Charron,
Novel brain wiring functions for classical morphogens: a role as graded positional cues in axon guidance.
2005,
Pubmed
Chhabra,
The many faces of actin: matching assembly factors with cellular structures.
2007,
Pubmed
Co,
Mechanism of actin network attachment to moving membranes: barbed end capture by N-WASP WH2 domains.
2007,
Pubmed
Dickson,
Molecular mechanisms of axon guidance.
2002,
Pubmed
Ding,
Molecular insights on context-specific role of profilin-1 in cell migration.
2012,
Pubmed
Fan,
Spatial coordination of actin polymerization and ILK-Akt2 activity during endothelial cell migration.
2009,
Pubmed
Fan,
Myo1c facilitates G-actin transport to the leading edge of migrating endothelial cells.
2012,
Pubmed
Gustafsson,
Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
2000,
Pubmed
Hatten,
Central nervous system neuronal migration.
1999,
Pubmed
Hitchcock,
Actin deoxyroboncuclease I interaction. Depolymerization and nucleotide exchange.
1980,
Pubmed
Johnson,
Neuronal IP3 3-kinase is an F-actin-bundling protein: role in dendritic targeting and regulation of spine morphology.
2009,
Pubmed
Khadka,
Non-redundant roles for Profilin2 and Profilin1 during vertebrate gastrulation.
2009,
Pubmed
,
Xenbase
Koestler,
F- and G-actin concentrations in lamellipodia of moving cells.
2009,
Pubmed
Lee,
Regulation of acetylcholine receptor clustering by ADF/cofilin-directed vesicular trafficking.
2009,
Pubmed
,
Xenbase
Lin,
Monoclonal antibodies against myofibrillar components of rat skeletal muscle decorate the intermediate filaments of cultured cells.
1981,
Pubmed
,
Xenbase
Lowery,
The trip of the tip: understanding the growth cone machinery.
2009,
Pubmed
,
Xenbase
Mannherz,
The beta-thymosins: intracellular and extracellular activities of a versatile actin binding protein family.
2009,
Pubmed
Matusek,
Formin proteins of the DAAM subfamily play a role during axon growth.
2008,
Pubmed
Melan,
Redistribution and differential extraction of soluble proteins in permeabilized cultured cells. Implications for immunofluorescence microscopy.
1992,
Pubmed
Ming,
Acute morphogenic and chemotropic effects of neurotrophins on cultured embryonic Xenopus spinal neurons.
1997,
Pubmed
,
Xenbase
Morton,
Latrunculin alters the actin-monomer subunit interface to prevent polymerization.
2000,
Pubmed
Munsie,
Lifeact cannot visualize some forms of stress-induced twisted F-actin.
2009,
Pubmed
Paavilainen,
Regulation of cytoskeletal dynamics by actin-monomer-binding proteins.
2004,
Pubmed
Peng,
Differential effects of neurotrophins and schwann cell-derived signals on neuronal survival/growth and synaptogenesis.
2003,
Pubmed
,
Xenbase
Pollard,
Cellular motility driven by assembly and disassembly of actin filaments.
2003,
Pubmed
Pollard,
Molecular mechanisms controlling actin filament dynamics in nonmuscle cells.
2000,
Pubmed
Qi,
Characterization of a CNS cell line, CAD, in which morphological differentiation is initiated by serum deprivation.
1997,
Pubmed
Riedl,
Lifeact: a versatile marker to visualize F-actin.
2008,
Pubmed
Rodriguez,
Conserved microtubule-actin interactions in cell movement and morphogenesis.
2003,
Pubmed
Roth,
beta-thymosin is required for axonal tract formation in developing zebrafish brain.
1999,
Pubmed
Sato,
Profilin is an effector for Daam1 in non-canonical Wnt signaling and is required for vertebrate gastrulation.
2006,
Pubmed
,
Xenbase
Schindelholz,
Quantitative estimation of F-actin in single growth cones.
1999,
Pubmed
Spector,
Latrunculins: novel marine toxins that disrupt microfilament organization in cultured cells.
1983,
Pubmed
Suter,
An emerging link between cytoskeletal dynamics and cell adhesion molecules in growth cone guidance.
1998,
Pubmed
Tessier-Lavigne,
The molecular biology of axon guidance.
1996,
Pubmed
Tsuji,
An order of magnitude faster AIP1-associated actin disruption than nucleation by the Arp2/3 complex in lamellipodia.
2009,
Pubmed
Van Baelen,
Vitamin D-binding protein (Gc-globulin) binds actin.
1980,
Pubmed
Van Goor,
The role of actin turnover in retrograde actin network flow in neuronal growth cones.
2012,
Pubmed
Vitriol,
Growth cone travel in space and time: the cellular ensemble of cytoskeleton, adhesion, and membrane.
2012,
Pubmed
Watanabe,
Single-molecule speckle analysis of actin filament turnover in lamellipodia.
2002,
Pubmed
,
Xenbase
Wen,
BMP gradients steer nerve growth cones by a balancing act of LIM kinase and Slingshot phosphatase on ADF/cofilin.
2007,
Pubmed
,
Xenbase
Wu,
Counting cytokinesis proteins globally and locally in fission yeast.
2005,
Pubmed
Yarmola,
Profilin: emerging concepts and lingering misconceptions.
2006,
Pubmed
Yarmola,
Actin-latrunculin A structure and function. Differential modulation of actin-binding protein function by latrunculin A.
2000,
Pubmed
Zicha,
Rapid actin transport during cell protrusion.
2003,
Pubmed
