David R and Wedlich D (2000), Xenopus cadherin-6 is expressed in the central ...

XB-ART-10196

Mech Dev 2000 Oct 01;971-2:187-90. doi: 10.1016/s0925-4773(00)00411-1.

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Xenopus cadherin-6 is expressed in the central and peripheral nervous system and in neurogenic placodes.

David R , Wedlich D .

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We have cloned the cDNAs encoding the two Xenopus laevis cadherin-6 pseudoalleles (Xcad-6 and Xcad-6'), which belong to the classical type II cadherin subfamily. During embryogenesis, Xcad-6 expression starts at early neurula and increases dramatically until tadpole stages. Whole-mount in situ hybridization revealed that the transcripts are localized in the developing brain and eye. However, most prominent expression was found in the peripheral nervous system, including a major proportion of lateral line structures. Our results implicate an important contribution of cadherin-6 to the formation of placode derived nerves in Xenopus, an observation which has not been described for other species.

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Species referenced: Xenopus laevis
Genes referenced: cad cdh6 gad1.2 rrad

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Fig. 2. Temporal and spatial expression of Xcad-6 mRNA. (A) RT-PCR analysis reveals Xcad-6 transcripts from early neurula stages onward. (B–D) Whole-mount in situ hybridizations of stage 26, 32 and 40 embryos. Xcad-6 mRNA is found in the brain, the eye (note expression in the developing retina (r) in C) and the PNS. (E) Transverse section of a stage 25 embryo showing Xcad-6 expression in the ganglion of facial, anteroventral and anterodorsal lateral line nerves. (F,G) Transverse sections of a stage 35 embryo: Xcad-6 is expressed in ganglia, roots and placodes of cranial and lateral line nerves. (H) Diagram showing Xcad-6 expression domains in neurogenic placodes (red) and in neural tissues (green) including brain, neural retina and peripheral nerves. A part of the anteroventral lateral line placode remains devoid of Xcad-6 mRNA (pink). e, eye anlage; epVII, epibranchial placode of facial nerve; epIX, epibranchial placode of glossopharyngeal nerve; epX1, epibranchial placode of first vagus nerve; epX2, X3, fused epibranchial placode of second and third vagus; fmb, forebrain-midbrain boundary; g, complex composed of the ganglia of glossopharyngeal and vagal as well as the middle and posterior dorsal lateral line nerves; gAD, AV, VII, fused ganglion of anterodorsal and anteroventral lateral line and facial nerves; Gad, ganglion of anterodorsal lateral line nerve; gVII, ganglion of facial nerve; gM, IX, fused ganglion of middle lateral line and glossopharyngeal nerves; gP, X, fused ganglion of posterior lateral line and vagus nerves; m, middle lateral line nerve; mhb, midbrain-hindbrain boundary; pAD, anterodorsal lateral line placode; pAV, anteroventral lateral line placode; pM, middle lateral line placode; pP, posterior lateral line placode; r, developing retina; rAD, AV, VII, root of anterodorsal and anteroventral lateral line and facial nerves; VII, facial nerve; IX, glossopharyngeal nerve; X1, X2, X3, three trunks of vagus nerve; vOT, otic vesicle.

Cdh6 expression at stage 32 using Whole-mount in situ hybridization in Xenopus laeveis