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EMBO J 2000 Sep 01;1917:4723-33. doi: 10.1093/emboj/19.17.4723.
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Multiple portions of poly(A)-binding protein stimulate translation in vivo.

Gray NK , Coller JM , Dickson KS , Wickens M .

Translational stimulation of mRNAs during early development is often accompanied by increases in poly(A) tail length. Poly(A)-binding protein (PAB) is an evolutionarily conserved protein that binds to the poly(A) tails of eukaryotic mRNAs. We examined PAB''s role in living cells, using both Xenopus laevis oocytes and Saccharomyces cerevisiae, by tethering it to the 3''-untranslated region of reporter mRNAs. Tethered PAB stimulates translation in vivo. Neither a poly(A) tail nor PAB''s poly(A)-binding activity is required. Multiple domains of PAB act redundantly in oocytes to stimulate translation: the interaction of RNA recognition motifs (RRMs) 1 and 2 with eukaryotic initiation factor-4G correlates with translational stimulation. Interaction with Paip-1 is insufficient for stimulation. RRMs 3 and 4 also stimulate, but bind neither factor. The regions of tethered PAB required in yeast to stimulate translation and stabilize mRNAs differ, implying that the two functions are distinct. Our results establish that oocytes contain the machinery necessary to support PAB-mediated translation and suggest that PAB may be an important participant in translational regulation during early development.

PubMed ID: 10970864
PMC ID: PMC302064
Article link: EMBO J
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: psip1

References [+] :
Amrani, Yeast Pab1 interacts with Rna15 and participates in the control of the poly(A) tail length in vitro. 1997, Pubmed