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Fig. 2. Expression of karyopherin-β3 in Xenopus embryos. Whole-mount in situ hybridisation with Xenopus karyopherin-β3 antisense RNA. (A) A frontal view and (B) a parasagittal section at the neural fold level (stage 16) show strong expression of karyopherin-β3 in the ea, eye-Anlage and weaker expression in the sln, sensorial layer of the neuroectoderm. (C) A lateral view of a stage 23 embryo reveals karyopherin-β3 transcripts in the ey, eye, ba, branchial arches and in the tb, tailbud. No expression is visible in the cg, cement gland. (D) A horizontal section of stage 23 shows strong expression in the eye and branchial arches. Lower expression levels are detectable in the mb, midbrain and tb, tailbud. (E) In a stage 29 embryo (also shown in F–J) karyopherin-β3 transcripts are localized in the eye, the branchial arches, the tt, tailtip and in the fore-/midbrain regions exept the pg, pineal gland. (F) In the parasagittal section of a late tailbud embryo, karyopherin-β3 expression is visible in the h, heart, tailtip, f, forebrain, midbrain and hm, head mesenchyme. No expression is detectable in the cement gland, n, notochord and en, endoderm. (G) The frontal section of the eye/midbrain level shows strong expression enhancement in the eyes and dorsal regions of the midbrain, a weaker signal is detected in the midbrain floor and hm, head mesenchym. No expression is visible in the cement gland, sa, stomodeal anlage and pineal gland. (H) Partial magnification of (G) shows a differential distribution of the midbrain and no signal in the ep, epidermis. (I) Frontal section just behind the head with strong expression in the nc, neural crest, scr, spinal chord roof, and lm, lateral mesoderm. (J) Posterior section of the embryo. The expression in neural crest cells becomes weak. Enhancement of expression is visible in the p, pronephros region and in the spinal cord roof, weak expression is detectable in the scf, spinal chord floor.
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Fig. 3. Common expression characteristics of ribosomal proteins and nuclear import factors. Whole-mount in situ hybridisation analysis was performed using antisense RNAs corresponding to the entire coding region for karyopherin-β3, RanBP7, RanBP1 and L5. Partial antisense sequences were used for in situ hybridization analysis of RanTC4 and S3A. The presented examples were grouped into three classes: transport factors (karyopherin-β3 and RanBP7), transport factor associated proteins (RanTC4 and RanBP1) and ribosomal proteins (L5 and S3A). Early tailtip stages (29–30) are shown in lateral view.
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Ipo5 (importin 5) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 28, lateral view, anterior left, dorsal up.
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ipo7 (importin 7) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 28, lateral view, anterior left, dorsal up.
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rpl5 (ribosomal protein L5 ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 27, lateral view, anterior left, dorsal up.
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ranbp1 (RAN binding protein 1 ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 28, lateral view, anterior left, dorsal up.
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ran (RAN, member RAS oncogene family ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 27, lateral view, anterior left, dorsal up.
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ipo5 ( importin 5 ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 16, anterior view,dorsal up.
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ipo5 ( importin 5 ) gene expression in bisected Xenopus laevis embryo, mid-sagittal section, assayed via in situ hybridization, NF stage 16, dorsal up, anterior left.
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ipo5 ( importin 5 ) gene expression in Xenopus laevis embryo, horizontal section, assayed via in situ hybridization, NF stage 23, anterior left.
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