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BDNF-Induced potentiation of spontaneous twitching in innervated myocytes requires calcium release from intracellular stores.
Kleiman RJ
,
Tian N
,
Krizaj D
,
Hwang TN
,
Copenhagen DR
,
Reichardt LF
.
???displayArticle.abstract??? Brain-derived neurotrophic factor (BDNF) can potentiate synaptic release at newly developed frog neuromuscular junctions. Although this potentiation depends on extracellular Ca(2+) and reflects changes in acetylcholine release, little is known about the intracellular transduction or calcium signaling pathways. We have developed a video assay for neurotrophin-induced potentiation of myocyte twitching as a measure of potentiation of synaptic activity. We use this assay to show that BDNF-induced synaptic potentiation is not blocked by cadmium, indicating that Ca(2+) influx through voltage-gated Ca(2+) channels is not required. TrkB autophosphorylation is not blocked in Ca(2+)-free conditions, indicating that TrkB activity is not Ca(2+) dependent. Additionally, an inhibitor of phospholipase C interferes with BDNF-induced potentiation. These results suggest that activation of the TrkB receptor activates phospholipase C to initiate intracellular Ca(2+) release from stores which subsequently potentiates transmitter release.
Barish,
Voltage-gated calcium currents in cultured embryonic Xenopus spinal neurones.
1991,
Pubmed
,
Xenbase
Barritt,
Receptor-activated Ca2+ inflow in animal cells: a variety of pathways tailored to meet different intracellular Ca2+ signalling requirements.
1999,
Pubmed
Berridge,
Neuronal calcium signaling.
1998,
Pubmed
Blöchl,
Characterization of nerve growth factor (NGF) release from hippocampal neurons: evidence for a constitutive and an unconventional sodium-dependent regulated pathway.
1995,
Pubmed
Boulanger,
Presynaptic depolarization facilitates neurotrophin-induced synaptic potentiation.
1999,
Pubmed
,
Xenbase
Cabelli,
Inhibition of ocular dominance column formation by infusion of NT-4/5 or BDNF.
1995,
Pubmed
Cabelli,
Blockade of endogenous ligands of trkB inhibits formation of ocular dominance columns.
1997,
Pubmed
Cohen-Cory,
Effects of brain-derived neurotrophic factor on optic axon branching and remodelling in vivo.
1995,
Pubmed
,
Xenbase
Fu,
ATP potentiates spontaneous transmitter release at developing neuromuscular synapses.
1991,
Pubmed
,
Xenbase
Gallin,
Calcium regulation of gene expression in neurons: the mode of entry matters.
1995,
Pubmed
Golovina,
Modulation of two functionally distinct Ca2+ stores in astrocytes: role of the plasmalemmal Na/Ca exchanger.
1996,
Pubmed
Gomez,
In vivo regulation of axon extension and pathfinding by growth-cone calcium transients.
1999,
Pubmed
,
Xenbase
Grynkiewicz,
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
1985,
Pubmed
Gu,
Distinct aspects of neuronal differentiation encoded by frequency of spontaneous Ca2+ transients.
1995,
Pubmed
,
Xenbase
Guiton,
Identification of in vivo brain-derived neurotrophic factor-stimulated autophosphorylation sites on the TrkB receptor tyrosine kinase by site-directed mutagenesis.
1994,
Pubmed
Guo,
Exocytosis coupled to mobilization of intracellular calcium by muscarine and caffeine in rat chromaffin cells.
1996,
Pubmed
Holm,
Activation of calcium-dependent potassium channels in mouse [correction of rat] brain neurons by neurotrophin-3 and nerve growth factor.
1997,
Pubmed
Huang,
Expression of Trk receptors in the developing mouse trigeminal ganglion: in vivo evidence for NT-3 activation of TrkA and TrkB in addition to TrkC.
1999,
Pubmed
Ip,
Similarities and differences in the way neurotrophins interact with the Trk receptors in neuronal and nonneuronal cells.
1993,
Pubmed
,
Xenbase
Kang,
Long-lasting neurotrophin-induced enhancement of synaptic transmission in the adult hippocampus.
1995,
Pubmed
Kano,
Ryanodine receptor-mediated intracellular calcium release in rat cerebellar Purkinje neurones.
1995,
Pubmed
Kaplan,
Signal transduction by the neurotrophin receptors.
1997,
Pubmed
Levine,
Brain-derived neurotrophic factor rapidly enhances synaptic transmission in hippocampal neurons via postsynaptic tyrosine kinase receptors.
1995,
Pubmed
Levine,
Brain-derived neurotrophic factor modulates hippocampal synaptic transmission by increasing N-methyl-D-aspartic acid receptor activity.
1998,
Pubmed
Li,
Activation of a TRPC3-dependent cation current through the neurotrophin BDNF.
1999,
Pubmed
Li,
Enhancement of neurotransmitter release induced by brain-derived neurotrophic factor in cultured hippocampal neurons.
1998,
Pubmed
Lockhart,
Nerve growth factor modulates synaptic transmission between sympathetic neurons and cardiac myocytes.
1997,
Pubmed
Lohof,
Potentiation of developing neuromuscular synapses by the neurotrophins NT-3 and BDNF.
1993,
Pubmed
,
Xenbase
Mahadeo,
High affinity nerve growth factor binding displays a faster rate of association than p140trk binding. Implications for multi-subunit polypeptide receptors.
1994,
Pubmed
Matthews,
Neurotransmitter release.
1996,
Pubmed
McAllister,
Neurotrophins regulate dendritic growth in developing visual cortex.
1995,
Pubmed
McAllister,
Opposing roles for endogenous BDNF and NT-3 in regulating cortical dendritic growth.
1997,
Pubmed
Merlio,
Increased production of the TrkB protein tyrosine kinase receptor after brain insults.
1993,
Pubmed
Meyer-Franke,
Depolarization and cAMP elevation rapidly recruit TrkB to the plasma membrane of CNS neurons.
1998,
Pubmed
Middlemas,
Identification of TrkB autophosphorylation sites and evidence that phospholipase C-gamma 1 is a substrate of the TrkB receptor.
1994,
Pubmed
Obermeier,
Tyrosine 785 is a major determinant of Trk--substrate interaction.
1993,
Pubmed
Patterson,
Recombinant BDNF rescues deficits in basal synaptic transmission and hippocampal LTP in BDNF knockout mice.
1996,
Pubmed
Peng,
Tissue culture of Xenopus neurons and muscle cells as a model for studying synaptic induction.
1991,
Pubmed
,
Xenbase
Sala,
Nerve growth factor and brain-derived neurotrophic factor increase neurotransmitter release in the rat visual cortex.
1998,
Pubmed
Schmidt-Kastner,
Cellular hybridization for BDNF, trkB, and NGF mRNAs and BDNF-immunoreactivity in rat forebrain after pilocarpine-induced status epilepticus.
1996,
Pubmed
Scott,
Aspects of vertebrate neuronal voltage-activated calcium currents and their regulation.
1991,
Pubmed
Shieh,
Identification of a signaling pathway involved in calcium regulation of BDNF expression.
1998,
Pubmed
Simpson,
Divalent cation entry in cultured rat cerebellar granule cells measured using Mn2+ quench of fura 2 fluorescence.
1995,
Pubmed
Smith,
Receptor-coupled signal transduction in human polymorphonuclear neutrophils: effects of a novel inhibitor of phospholipase C-dependent processes on cell responsiveness.
1990,
Pubmed
Stoop,
Potentiation of transmitter release by ciliary neurotrophic factor requires somatic signaling.
1995,
Pubmed
,
Xenbase
Stoop,
Synaptic modulation by neurotrophic factors: differential and synergistic effects of brain-derived neurotrophic factor and ciliary neurotrophic factor.
1996,
Pubmed
,
Xenbase
Tanaka,
Inhibition of GABAA synaptic responses by brain-derived neurotrophic factor (BDNF) in rat hippocampus.
1997,
Pubmed
Tao,
Ca2+ influx regulates BDNF transcription by a CREB family transcription factor-dependent mechanism.
1998,
Pubmed
Treiman,
A tool coming of age: thapsigargin as an inhibitor of sarco-endoplasmic reticulum Ca(2+)-ATPases.
1998,
Pubmed
Tse,
Local Ca2+ release from internal stores controls exocytosis in pituitary gonadotrophs.
1997,
Pubmed
Wang,
Potentiation of developing synapses by postsynaptic release of neurotrophin-4.
1997,
Pubmed
,
Xenbase
Xie,
Activity-dependent expression of NT-3 in muscle cells in culture: implications in the development of neuromuscular junctions.
1997,
Pubmed
,
Xenbase
Zhou,
BDNF/NT4-5 receptor TrkB and cadherin participate in cell-cell adhesion.
1997,
Pubmed
