Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-11413
Science 2000 Mar 03;2875458:1644-7.
Show Gene links Show Anatomy links

Requirement of the prolyl isomerase Pin1 for the replication checkpoint.

Winkler KE , Swenson KI , Kornbluth S , Means AR .


???displayArticle.abstract???
The peptidyl-prolyl isomerase Pin1 has been implicated in regulating cell cycle progression. Pin1 was found to be required for the DNA replication checkpoint in Xenopus laevis. Egg extracts depleted of Pin1 inappropriately transited from the G2 to the M phase of the cell cycle in the presence of the DNA replication inhibitor aphidicolin. This defect in replication checkpoint function was reversed after the addition of recombinant wild-type Pin1, but not an isomerase-inactive mutant, to the depleted extract. Premature mitotic entry in the absence of Pin1 was accompanied by hyperphosphorylation of Cdc25, activation of Cdc2/cyclin B, and generation of epitopes recognized by the mitotic phosphoprotein antibody, MPM-2. Therefore, Pin1 appears to be required for the checkpoint delaying the onset of mitosis in response to incomplete replication.

???displayArticle.pubmedLink??? 10698738
???displayArticle.link??? Science
???displayArticle.grants??? [+]

Species referenced: Xenopus laevis
Genes referenced: pin1 rasgrf1