Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1999 Dec 01;1912:8591-603. doi: 10.1128/MCB.19.12.8591.
Show Gene links
Show Anatomy links
NF-Y associates with H3-H4 tetramers and octamers by multiple mechanisms.
Caretti G
,
Motta MC
,
Mantovani R
.
???displayArticle.abstract???
NF-Y is a CCAAT-binding trimer with two histonic subunits, NF-YB and NF-YC, resembling H2A-H2B. We previously showed that the short conserved domains of NF-Y efficiently bind to the major histocompatibility complex class II Ea Y box in DNA nucleosomized with purified chicken histones. Using wild-type NF-Y and recombinant histones, we find that NF-Y associates with H3-H4 early during nucleosome assembly, under conditions in which binding to naked DNA is not observed. In such assays, the NF-YB-NF-YC dimer forms complexes with H3-H4, for whose formation the CCAAT box is not required. We investigated whether they represent octamer-like structures, using DNase I, micrococcal nuclease, and exonuclease III, and found a highly positioned nucleosome on Ea, whose boundaries were mapped; addition of NF-YB-NF-YC does not lead to the formation of octameric structures, but changes in the digestion patterns are observed. NF-YA can bind to such preformed DNA complexes in a CCAAT-dependent way. In the absence of DNA, NF-YB-NF-YC subunits bind to H3-H4, but not to H2A-H2B, through the NF-YB histone fold. These results indicate that (i) the NF-Y histone fold dimer can efficiently associate DNA during nucleosome formation; (ii) it has an intrinsic affinity for H3-H4 but does not form octamers; and (iii) the interactions between NF-YA, NF-YB-NF-YC, and H3-H4 or nucleosomes are not mutually exclusive. Thus, NF-Y can intervene at different steps during nucleosome formation, and this scenario might be paradigmatic for other histone fold proteins involved in gene regulation.
Alevizopoulos,
A proline-rich TGF-beta-responsive transcriptional activator interacts with histone H3.
1995, Pubmed
Alevizopoulos,
A proline-rich TGF-beta-responsive transcriptional activator interacts with histone H3.
1995,
Pubmed
Arents,
The histone fold: a ubiquitous architectural motif utilized in DNA compaction and protein dimerization.
1995,
Pubmed
Baxevanis,
A variety of DNA-binding and multimeric proteins contain the histone fold motif.
1995,
Pubmed
Bellorini,
CCAAT binding NF-Y-TBP interactions: NF-YB and NF-YC require short domains adjacent to their histone fold motifs for association with TBP basic residues.
1997,
Pubmed
Bellorini,
Cloning and expression of human NF-YC.
1997,
Pubmed
Birck,
Human TAF(II)28 and TAF(II)18 interact through a histone fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family.
1998,
Pubmed
Bucher,
Weight matrix descriptions of four eukaryotic RNA polymerase II promoter elements derived from 502 unrelated promoter sequences.
1990,
Pubmed
Burley,
Biochemistry and structural biology of transcription factor IID (TFIID).
1996,
Pubmed
Chang,
Human thymidine kinase CCAAT-binding protein is NF-Y, whose A subunit expression is serum-dependent in human IMR-90 diploid fibroblasts.
1994,
Pubmed
Currie,
NF-Y is associated with the histone acetyltransferases GCN5 and P/CAF.
1998,
Pubmed
Dong,
Nucleosome positioning is determined by the (H3-H4)2 tetramer.
1991,
Pubmed
Edmondson,
Repression domain of the yeast global repressor Tup1 interacts directly with histones H3 and H4.
1996,
Pubmed
Farina,
Down-regulation of cyclin B1 gene transcription in terminally differentiated skeletal muscle cells is associated with loss of functional CCAAT-binding NF-Y complex.
1999,
Pubmed
Goppelt,
A mechanism for repression of class II gene transcription through specific binding of NC2 to TBP-promoter complexes via heterodimeric histone fold domains.
1996,
Pubmed
Hansen,
A role for histones H2A/H2B in chromatin folding and transcriptional repression.
1994,
Pubmed
,
Xenbase
Hayes,
Histone contributions to the structure of DNA in the nucleosome.
1991,
Pubmed
,
Xenbase
Hayes,
Histones H2A/H2B inhibit the interaction of transcription factor IIIA with the Xenopus borealis somatic 5S RNA gene in a nucleosome.
1992,
Pubmed
,
Xenbase
Hooft van Huijsduijnen,
Properties of a CCAAT box-binding protein.
1987,
Pubmed
Jin,
Transcriptional regulation of the MDR1 gene by histone acetyltransferase and deacetylase is mediated by NF-Y.
1998,
Pubmed
Kim,
Determination of functional domains in the C subunit of the CCAAT-binding factor (CBF) necessary for formation of a CBF-DNA complex: CBF-B interacts simultaneously with both the CBF-A and CBF-C subunits to form a heterotrimeric CBF molecule.
1996,
Pubmed
Kouzarides,
Histone acetylases and deacetylases in cell proliferation.
1999,
Pubmed
Landsberger,
Role of chromatin and Xenopus laevis heat shock transcription factor in regulation of transcription from the X. laevis hsp70 promoter in vivo.
1995,
Pubmed
,
Xenbase
Lee,
A positive role for histone acetylation in transcription factor access to nucleosomal DNA.
1993,
Pubmed
,
Xenbase
Lefebvre,
Binding of retinoic acid receptor heterodimers to DNA. A role for histones NH2 termini.
1998,
Pubmed
Li,
Xenopus NF-Y pre-sets chromatin to potentiate p300 and acetylation-responsive transcription from the Xenopus hsp70 promoter in vivo.
1998,
Pubmed
,
Xenbase
Liberati,
NF-Y organizes the gamma-globin CCAAT boxes region.
1998,
Pubmed
Liberati,
NF-Y binding to twin CCAAT boxes: role of Q-rich domains and histone fold helices.
1999,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Luger,
Characterization of nucleosome core particles containing histone proteins made in bacteria.
1997,
Pubmed
,
Xenbase
Maity,
Role of the CCAAT-binding protein CBF/NF-Y in transcription.
1998,
Pubmed
Mantovani,
A survey of 178 NF-Y binding CCAAT boxes.
1998,
Pubmed
Mantovani,
Dominant negative analogs of NF-YA.
1994,
Pubmed
Marziali,
The activity of the CCAAT-box binding factor NF-Y is modulated through the regulated expression of its A subunit during monocyte to macrophage differentiation: regulation of tissue-specific genes through a ubiquitous transcription factor.
1999,
Pubmed
Masternak,
A gene encoding a novel RFX-associated transactivator is mutated in the majority of MHC class II deficiency patients.
1998,
Pubmed
Motta,
Interactions of the CCAAT-binding trimer NF-Y with nucleosomes.
1999,
Pubmed
Ogryzko,
Histone-like TAFs within the PCAF histone acetylase complex.
1998,
Pubmed
Perry,
Histone acetylation increases the solubility of chromatin and occurs sequentially over most of the chromatin. A novel model for the biological role of histone acetylation.
1982,
Pubmed
Scholz,
Hormone-dependent recruitment of NF-Y to the uteroglobin gene enhancer associated with chromatin remodeling in rabbit endometrial epithelium.
1999,
Pubmed
Sinha,
Three classes of mutations in the A subunit of the CCAAT-binding factor CBF delineate functional domains involved in the three-step assembly of the CBF-DNA complex.
1996,
Pubmed
Spangenberg,
The mouse mammary tumour virus promoter positioned on a tetramer of histones H3 and H4 binds nuclear factor 1 and OTF1.
1998,
Pubmed
Tse,
Disruption of higher-order folding by core histone acetylation dramatically enhances transcription of nucleosomal arrays by RNA polymerase III.
1998,
Pubmed
,
Xenbase
Vettese-Dadey,
Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro.
1996,
Pubmed
Viville,
The E alpha promoter: a linker-scanning analysis.
1991,
Pubmed
Workman,
Alteration of nucleosome structure as a mechanism of transcriptional regulation.
1998,
Pubmed
Xing,
Mutations in yeast HAP2/HAP3 define a hybrid CCAAT box binding domain.
1993,
Pubmed
Zemzoumi,
NF-Y histone fold alpha1 helices help impart CCAAT specificity.
1999,
Pubmed
de Silvio,
Dissection of the NF-Y transcriptional activation potential.
1999,
Pubmed