Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Genes Dev
2005 Nov 01;1921:2583-92. doi: 10.1101/gad.1371105.
Show Gene links
Show Anatomy links
Symplekin and multiple other polyadenylation factors participate in 3'-end maturation of histone mRNAs.
Kolev NG
,
Steitz JA
.
???displayArticle.abstract???
Most metazoan messenger RNAs encoding histones are cleaved, but not polyadenylated at their 3' ends. Processing in mammalian cell extracts requires the U7 small nuclear ribonucleoprotein (U7 snRNP) and an unidentified heat-labile factor (HLF). We describe the identification of a heat-sensitive protein complex whose integrity is required for histone pre-mRNA cleavage. It includes all five subunits of the cleavage and polyadenylation specificity factor (CPSF), two subunits of the cleavage stimulation factor (CstF), and symplekin. Reconstitution experiments reveal that symplekin, previously shown to be necessary for cytoplasmic poly(A) tail elongation and translational activation of mRNAs during Xenopus oocyte maturation, is the essential heat-labile component. Thus, a common molecular machinery contributes to the nuclear maturation of mRNAs both lacking and possessing poly(A), as well as to cytoplasmic poly(A) tail elongation.
???displayArticle.pubmedLink???
16230528
???displayArticle.pmcLink???PMC1276732 ???displayArticle.link???Genes Dev ???displayArticle.grants???[+]
Abbott,
The stem-loop binding protein (SLBP1) is present in coiled bodies of the Xenopus germinal vesicle.
1999, Pubmed,
Xenbase
Abbott,
The stem-loop binding protein (SLBP1) is present in coiled bodies of the Xenopus germinal vesicle.
1999,
Pubmed
,
Xenbase
Barabino,
The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins.
1997,
Pubmed
Barnard,
Symplekin and xGLD-2 are required for CPEB-mediated cytoplasmic polyadenylation.
2004,
Pubmed
,
Xenbase
Bienroth,
Purification of the cleavage and polyadenylation factor involved in the 3'-processing of messenger RNA precursors.
1991,
Pubmed
Birchmeier,
3' editing of mRNAs: sequence requirements and involvement of a 60-nucleotide RNA in maturation of histone mRNA precursors.
1984,
Pubmed
,
Xenbase
Bond,
Multiple processing-defective mutations in a mammalian histone pre-mRNA are suppressed by compensatory changes in U7 RNA both in vivo and in vitro.
1991,
Pubmed
Callan,
Histone genes are located at the sphere loci of Xenopus lampbrush chromosomes.
1991,
Pubmed
,
Xenbase
Callebaut,
Metallo-beta-lactamase fold within nucleic acids processing enzymes: the beta-CASP family.
2002,
Pubmed
Dominski,
A novel zinc finger protein is associated with U7 snRNP and interacts with the stem-loop binding protein in the histone pre-mRNP to stimulate 3'-end processing.
2002,
Pubmed
,
Xenbase
Dominski,
Stem-loop binding protein facilitates 3'-end formation by stabilizing U7 snRNP binding to histone pre-mRNA.
1999,
Pubmed
Dominski,
The polyadenylation factor CPSF-73 is involved in histone-pre-mRNA processing.
2005,
Pubmed
Eliassen,
Role for a YY1-binding element in replication-dependent mouse histone gene expression.
1998,
Pubmed
Frey,
Coiled bodies contain U7 small nuclear RNA and associate with specific DNA sequences in interphase human cells.
1995,
Pubmed
Gall,
Assembly of the nuclear transcription and processing machinery: Cajal bodies (coiled bodies) and transcriptosomes.
1999,
Pubmed
,
Xenbase
Gall,
Histone genes are located at the sphere loci of newt lampbrush chromosomes.
1981,
Pubmed
Gick,
Generation of histone mRNA 3' ends by endonucleolytic cleavage of the pre-mRNA in a snRNP-dependent in vitro reaction.
1986,
Pubmed
Gick,
Heat-labile regulatory factor is required for 3' processing of histone precursor mRNAs.
1987,
Pubmed
Gilmartin,
Eukaryotic mRNA 3' processing: a common means to different ends.
2005,
Pubmed
Groisman,
Translational control of the embryonic cell cycle.
2002,
Pubmed
,
Xenbase
Hofmann,
Symplekin, a constitutive protein of karyo- and cytoplasmic particles involved in mRNA biogenesis in Xenopus laevis oocytes.
2002,
Pubmed
,
Xenbase
Huang,
N-methyl-D-aspartate receptor signaling results in Aurora kinase-catalyzed CPEB phosphorylation and alpha CaMKII mRNA polyadenylation at synapses.
2002,
Pubmed
,
Xenbase
Jenny,
Characterization of cleavage and polyadenylation specificity factor and cloning of its 100-kilodalton subunit.
1994,
Pubmed
Jenny,
Sequence similarity between the 73-kilodalton protein of mammalian CPSF and a subunit of yeast polyadenylation factor I.
1996,
Pubmed
Kambach,
Crystal structures of two Sm protein complexes and their implications for the assembly of the spliceosomal snRNPs.
1999,
Pubmed
Kaufmann,
Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase.
2004,
Pubmed
Keon,
Symplekin, a novel type of tight junction plaque protein.
1996,
Pubmed
Krieg,
Formation of the 3' end of histone mRNA by post-transcriptional processing.
,
Pubmed
,
Xenbase
Lambright,
The 2.0 A crystal structure of a heterotrimeric G protein.
1996,
Pubmed
Last,
Multiple interactions of the transcription factor YY1 with human histone H4 gene regulatory elements.
1999,
Pubmed
Lüscher,
RNA 3' processing regulates histone mRNA levels in a mammalian cell cycle mutant. A processing factor becomes limiting in G1-arrested cells.
1987,
Pubmed
Martincic,
Increase in the 64-kDa subunit of the polyadenylation/cleavage stimulatory factor during the G0 to S phase transition.
1998,
Pubmed
Marzluff,
Histone mRNA expression: multiple levels of cell cycle regulation and important developmental consequences.
2002,
Pubmed
Melin,
Biochemical demonstration of complex formation of histone pre-mRNA with U7 small nuclear ribonucleoprotein and hairpin binding factors.
1992,
Pubmed
Mowry,
Identification of the human U7 snRNP as one of several factors involved in the 3' end maturation of histone premessenger RNA's.
1987,
Pubmed
Murthy,
Characterization of the multisubunit cleavage-polyadenylation specificity factor from calf thymus.
1992,
Pubmed
Murthy,
The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation.
1995,
Pubmed
Pillai,
Purified U7 snRNPs lack the Sm proteins D1 and D2 but contain Lsm10, a new 14 kDa Sm D1-like protein.
2001,
Pubmed
Pillai,
Unique Sm core structure of U7 snRNPs: assembly by a specialized SMN complex and the role of a new component, Lsm11, in histone RNA processing.
2003,
Pubmed
Price,
Fractionation of transcription factors for RNA polymerase II from Drosophila Kc cell nuclear extracts.
1987,
Pubmed
Raker,
Spliceosomal U snRNP core assembly: Sm proteins assemble onto an Sm site RNA nonanucleotide in a specific and thermodynamically stable manner.
1999,
Pubmed
Ryan,
Evidence that polyadenylation factor CPSF-73 is the mRNA 3' processing endonuclease.
2004,
Pubmed
Rüegsegger,
Human pre-mRNA cleavage factor Im is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits.
1998,
Pubmed
Scharl,
Length suppression in histone messenger RNA 3'-end maturation: processing defects of insertion mutant premessenger RNAs can be compensated by insertions into the U7 small nuclear RNA.
1996,
Pubmed
,
Xenbase
Scharl,
The site of 3' end formation of histone messenger RNA is a fixed distance from the downstream element recognized by the U7 snRNP.
1994,
Pubmed
Schaufele,
Compensatory mutations suggest that base-pairing with a small nuclear RNA is required to form the 3' end of H3 messenger RNA.
,
Pubmed
,
Xenbase
Schul,
The RNA 3' cleavage factors CstF 64 kDa and CPSF 100 kDa are concentrated in nuclear domains closely associated with coiled bodies and newly synthesized RNA.
1996,
Pubmed
Schul,
Nuclear domains enriched in RNA 3'-processing factors associate with coiled bodies and histone genes in a cell cycle-dependent manner.
1999,
Pubmed
Sheets,
Products of in vitro cleavage and polyadenylation of simian virus 40 late pre-mRNAs.
1987,
Pubmed
Shi,
Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein.
1991,
Pubmed
Sondek,
Crystal structure of a G-protein beta gamma dimer at 2.1A resolution.
1996,
Pubmed
Streit,
Variable effects of the conserved RNA hairpin element upon 3' end processing of histone pre-mRNA in vitro.
1993,
Pubmed
Strub,
The cDNA sequences of the sea urchin U7 small nuclear RNA suggest specific contacts between histone mRNA precursor and U7 RNA during RNA processing.
1984,
Pubmed
,
Xenbase
Takagaki,
Four factors are required for 3'-end cleavage of pre-mRNAs.
1989,
Pubmed
Takagaki,
A multisubunit factor, CstF, is required for polyadenylation of mammalian pre-mRNAs.
1990,
Pubmed
Takagaki,
The human 64-kDa polyadenylylation factor contains a ribonucleoprotein-type RNA binding domain and unusual auxiliary motifs.
1992,
Pubmed
Takagaki,
A human polyadenylation factor is a G protein beta-subunit homologue.
1992,
Pubmed
Takagaki,
A polyadenylation factor subunit is the human homologue of the Drosophila suppressor of forked protein.
1994,
Pubmed
Takagaki,
RNA recognition by the human polyadenylation factor CstF.
1997,
Pubmed
Takagaki,
Complex protein interactions within the human polyadenylation machinery identify a novel component.
2000,
Pubmed
Wall,
The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma 2.
1995,
Pubmed
Wu,
U7 small nuclear RNA in C snurposomes of the Xenopus germinal vesicle.
1993,
Pubmed
,
Xenbase
Wu,
CPEB-mediated cytoplasmic polyadenylation and the regulation of experience-dependent translation of alpha-CaMKII mRNA at synapses.
1998,
Pubmed
,
Xenbase
Xing,
HSF1 modulation of Hsp70 mRNA polyadenylation via interaction with symplekin.
2004,
Pubmed
de Vries,
Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors.
2000,
Pubmed
