XB-ART-12513
Biochim Biophys Acta
1999 Aug 17;14331-2:343-9.
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Molecular cloning of a cold-shock domain protein, zfY1, in zebrafish embryo(1).
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Cold-shock domain proteins in vertebrates contain a highly conserved domain which is related to the Escherichia coli cold-shock proteins. Here we report the cloning of a cold-shock domain protein from zebrafish embryo. Using the combination of PCR techniques with degenerate primers, 5'RACE and 3'RACE, the full length cDNA of a cold-shock domain protein in the zebrafish embryo was successfully cloned without constructing and screening a library. Determined from the deduced amino acid sequence, this protein is most similar to Xenopus, FRGY1, and this newly cloned zebrafish gene was therefore designated as zfY1.
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Species referenced: Xenopus
Genes referenced: ybx1