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Proc Natl Acad Sci U S A 1998 Dec 08;9525:14693-8. doi: 10.1073/pnas.95.25.14693.
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Protein phosphatase 2A is required for the initiation of chromosomal DNA replication.

Lin XH , Walter J , Scheidtmann K , Ohst K , Newport J , Walter G .

Protein phosphatase 2A (PP2A) is an abundant, multifunctional serine/threonine-specific phosphatase that stimulates simian virus 40 DNA replication. The question as to whether chromosomal DNA replication also depends on PP2A was addressed by using a cell-free replication system derived from Xenopus laevis eggs. Immunodepletion of PP2A from Xenopus egg extract resulted in strong inhibition of DNA replication. PP2A was required for the initiation of replication but not for the elongation of previously engaged replication forks. Therefore, the initiation of chromosomal DNA replication depends not only on phosphorylation by protein kinases but also on dephosphorylation by PP2A.

PubMed ID: 9843951
PMC ID: PMC24511
Article link: Proc Natl Acad Sci U S A
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: ptpa

References [+] :
Adachi, Study of the cell cycle-dependent assembly of the DNA pre-replication centres in Xenopus egg extracts. 1994, Pubmed, Xenbase