Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Biochem J
2006 Jan 01;393Pt 1:267-75. doi: 10.1042/BJ20050920.
Show Gene links
Show Anatomy links
ScOPT1 and AtOPT4 function as proton-coupled oligopeptide transporters with broad but distinct substrate specificities.
Osawa H
,
Stacey G
,
Gassmann W
.
???displayArticle.abstract???
A group of OPTs (oligopeptide transporters) exclusively identified in plants and fungi are proposed to transport oligopeptides and derivatives of three to six amino acids in length, but their transport mechanisms and biological functions are poorly understood. We expressed the Saccharomyces cerevisiae (yeast) OPT ScOPT1 and five Arabidopsis thaliana AtOPTs in Xenopus laevis oocytes for two-electrode voltage-clamp studies. ScOPT1 produced inward currents in response to GSH or GSSG, the phytochelatin (PC) PC2 and oligopeptides including the tetrapeptide GGFL, but not KLGL. Inward currents were dependent on the external proton and substrate concentrations, with high affinity for both. This and the inward currents evoked by substrates with net negative charges showed that ScOPT1 is a proton-coupled transporter. ScOPT1 displayed highest apparent affinity for PC2, with small differences in the maximal current among substrates. Glutathione transport by any of the tested AtOPTs, including AtOPT6, was not detected in yeast growth complementation assays. With AtOPT4, initially only small KLGL-dependent currents were recorded in batches of oocytes showing high ScOPT1 expression. AtOPT4 expression was optimized by swapping the 5'-untranslated region with that of ScOPT1. AtOPT4 displayed a higher affinity for KLGL than ScOPT1 did for any peptide. AtOPT4-mediated KLGL transport was detectable at pH 5.0, but not at pH 6.0 or 7.0. Taken together, our results demonstrate that ScOPT1 and AtOPT4 are proton-coupled OPTs with broad but distinct substrate specificities and affinities.
Anderson,
Determination of glutathione and glutathione disulfide in biological samples.
1985, Pubmed
Anderson,
Determination of glutathione and glutathione disulfide in biological samples.
1985,
Pubmed
Becker,
A cDNA encoding a human CCAAT-binding protein cloned by functional complementation in yeast.
1991,
Pubmed
Boorer,
Kinetics and specificity of a H+/amino acid transporter from Arabidopsis thaliana.
1996,
Pubmed
,
Xenbase
Boorer,
Specificity and stoichiometry of the Arabidopsis H+/amino acid transporter AAP5.
1997,
Pubmed
,
Xenbase
Bourbouloux,
Hgt1p, a high affinity glutathione transporter from the yeast Saccharomyces cerevisiae.
2000,
Pubmed
Brachmann,
Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications.
1998,
Pubmed
Cagnac,
AtOPT6 transports glutathione derivatives and is induced by primisulfuron.
2004,
Pubmed
Chaudhuri,
apd1+, a gene required for red pigment formation in ade6 mutants of Schizosaccharomyces pombe, encodes an enzyme required for glutathione biosynthesis: a role for glutathione and a glutathione-conjugate pump.
1997,
Pubmed
Chen,
Stoichiometry and kinetics of the high-affinity H+-coupled peptide transporter PepT2.
1999,
Pubmed
,
Xenbase
Clemens,
Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast.
1999,
Pubmed
Cobbett,
Phytochelatin biosynthesis and function in heavy-metal detoxification.
2000,
Pubmed
Curie,
Maize yellow stripe1 encodes a membrane protein directly involved in Fe(III) uptake.
2001,
Pubmed
DiDonato,
Arabidopsis Yellow Stripe-Like2 (YSL2): a metal-regulated gene encoding a plasma membrane transporter of nicotianamine-metal complexes.
2004,
Pubmed
Fei,
A novel H(+)-coupled oligopeptide transporter (OPT3) from Caenorhabditis elegans with a predominant function as a H(+) channel and an exclusive expression in neurons.
2000,
Pubmed
,
Xenbase
Gunshin,
Cloning and characterization of a mammalian proton-coupled metal-ion transporter.
1997,
Pubmed
,
Xenbase
Ha,
Phytochelatin synthase genes from Arabidopsis and the yeast Schizosaccharomyces pombe.
1999,
Pubmed
Hauser,
Enkephalins are transported by a novel eukaryotic peptide uptake system.
2000,
Pubmed
Koh,
An oligopeptide transporter gene family in Arabidopsis.
2002,
Pubmed
Kottra,
PEPT1 as a paradigm for membrane carriers that mediate electrogenic bidirectional transport of anionic, cationic, and neutral substrates.
2002,
Pubmed
,
Xenbase
Kozak,
At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cells.
1987,
Pubmed
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
Lubkowitz,
An oligopeptide transport gene from Candida albicans.
1997,
Pubmed
Lubkowitz,
Schizosaccharomyces pombe isp4 encodes a transporter representing a novel family of oligopeptide transporters.
1998,
Pubmed
Schaaf,
ZmYS1 functions as a proton-coupled symporter for phytosiderophore- and nicotianamine-chelated metals.
2004,
Pubmed
,
Xenbase
Srikanth,
Multiple cis-regulatory elements and the yeast sulphur regulatory network are required for the regulation of the yeast glutathione transporter, Hgt1p.
2005,
Pubmed
Stacey,
Peptide transport in plants.
2002,
Pubmed
Stacey,
AtOPT3, a member of the oligopeptide transporter family, is essential for embryo development in Arabidopsis.
2002,
Pubmed
Stacey,
Expression analyses of Arabidopsis oligopeptide transporters during seed germination, vegetative growth and reproduction.
2006,
Pubmed
Sze,
Energization of plant cell membranes by H+-pumping ATPases. Regulation and biosynthesis.
1999,
Pubmed
Vatamaniuk,
AtPCS1, a phytochelatin synthase from Arabidopsis: isolation and in vitro reconstitution.
1999,
Pubmed
Yen,
Maize Yellow Stripe1, an iron-phytosiderophore uptake transporter, is a member of the oligopeptide transporter (OPT) family.
2001,
Pubmed
Zenk,
Heavy metal detoxification in higher plants--a review.
1996,
Pubmed
Zhang,
A novel family of transporters mediating the transport of glutathione derivatives in plants.
2004,
Pubmed
