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Dev Biol 1998 Oct 01;2021:113-24. doi: 10.1006/dbio.1998.8982.
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Voltage-dependent activation of frog eggs by a sperm surface disintegrin peptide.

Shilling FM , Magie CR , Nuccitelli R .

Fertilin, a sperm protein of the metalloprotease/disintegrin/cysteine-rich (MDC) family, plays a critical role in sperm-egg binding in mammals. Peptides corresponding to the disintegrin domain of fertilin and antibodies against fertilin have been shown to inhibit mammalian sperm-egg binding and fusion. A protein from the same family, xMDC16, was recently cloned from frog (Xenopus laevis) testis and was found to be involved in frog sperm-egg binding. Here we report that xMDC16 is localized predominantly on the posterior surface of egg jelly-activated sperm, and peptides from the disintegrin domain of this protein activate eggs when applied near the egg surface. Egg activation was dependent on (1) specific amino acid residues (KTX); (2) the presence of divalent cations, but not external Ca2+ alone; and (3) voltage across the egg plasma membrane. This is the first demonstration of egg activation in vertebrates by the surface application of a peptide derived from a sperm surface protein, supporting a model for egg activation that involves a signal transducing receptor for sperm in the egg''s plasma membrane.

PubMed ID: 9758707
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: adam21 adam28.2 gopc mbp slc5a5

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