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J Physiol
2005 Nov 01;568Pt 3:767-88. doi: 10.1113/jphysiol.2005.087858.
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Multiprotein assembly of Kv4.2, KChIP3 and DPP10 produces ternary channel complexes with ISA-like properties.
Jerng HH
,
Kunjilwar K
,
Pfaffinger PJ
.
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Kv4 pore-forming subunits are the principal constituents of the voltage-gated K+ channel underlying somatodendritic subthreshold A-type currents (I(SA)) in neurones. Two structurally distinct types of Kv4 channel modulators, Kv channel-interacting proteins (KChIPs) and dipeptidyl-peptidase-like proteins (DPLs: DPP6 or DPPX, DPP10 or DPPY), enhance surface expression and modify functional properties. Since KChIP and DPL distributions overlap in the brain, we investigated the potential coassembly of Kv4.2, KChIP3 and DPL proteins, and the contribution of DPLs to ternary complex properties. Immunoprecipitation results show that KChIP3 and DPP10 associate simultaneously with Kv4.2 proteins in rat brain as well as heterologously expressing Xenopus oocytes, indicating Kv4.2 + KChIP3 + DPP10 multiprotein complexes. Consistent with ternary complex formation, coexpression of Kv4.2, KChIP3 and DPP10 in oocytes and CHO cells results in current waveforms distinct from the arithmetic sum of Kv4.2 + KChIP3 and Kv4.2 + DPP10 currents. Furthermore, the Kv4.2 + KChIP3 + DPP10 channels recover from inactivation very rapidly (tau(rec) approximately 18-26 ms), closely matching that of native I(SA) and significantly faster than the recovery of Kv4.2 + KChIP3 or Kv4.2 + DPP10 channels. For comparison, identical triple coexpression experiments were performed using DPP6 variants. While most results are similar, the Kv4.2 + KChIP3 + DPP6 channels exhibit inactivation that slows with increasing membrane potential, resulting in inactivation slower than that of Kv4.2 + KChIP3 + DPP10 channels at positive voltages. In conclusion, the native neuronal subthreshold A-type channel is probably a macromolecular complex formed from Kv4 and a combination of both KChIP and DPL proteins, with the precise composition of channel alpha and auxiliary subunits underlying tissue and regional variability in I(SA) properties.
Allen,
Positional cloning of a novel gene influencing asthma from chromosome 2q14.
2003, Pubmed
Allen,
Positional cloning of a novel gene influencing asthma from chromosome 2q14.
2003,
Pubmed
An,
Modulation of A-type potassium channels by a family of calcium sensors.
2000,
Pubmed
,
Xenbase
Banks,
Layer-specific properties of the transient K current (IA) in piriform cortex.
1996,
Pubmed
Baranauskas,
Cell-type-specific splicing of KChIP4 mRNA correlates with slower kinetics of A-type current.
2004,
Pubmed
,
Xenbase
Bardoni,
Kinetic study and numerical reconstruction of A-type current in granule cells of rat cerebellar slices.
1993,
Pubmed
Barrett,
The MEROPS database as a protease information system.
2001,
Pubmed
Baxter,
Ionic conductance mechanisms contributing to the electrophysiological properties of neurons.
1991,
Pubmed
Beck,
Remodelling inactivation gating of Kv4 channels by KChIP1, a small-molecular-weight calcium-binding protein.
2002,
Pubmed
,
Xenbase
Bekkers,
Distribution and activation of voltage-gated potassium channels in cell-attached and outside-out patches from large layer 5 cortical pyramidal neurons of the rat.
2000,
Pubmed
Bernard,
Acquired dendritic channelopathy in temporal lobe epilepsy.
2004,
Pubmed
Boland,
Functional properties of a brain-specific NH2-terminally spliced modulator of Kv4 channels.
2003,
Pubmed
,
Xenbase
Buxbaum,
Calsenilin: a calcium-binding protein that interacts with the presenilins and regulates the levels of a presenilin fragment.
1998,
Pubmed
Bähring,
Conserved Kv4 N-terminal domain critical for effects of Kv channel-interacting protein 2.2 on channel expression and gating.
2001,
Pubmed
Bähring,
Kinetic analysis of open- and closed-state inactivation transitions in human Kv4.2 A-type potassium channels.
2001,
Pubmed
Carrión,
DREAM is a Ca2+-regulated transcriptional repressor.
1999,
Pubmed
Castro,
Hippocampal heterotopia lack functional Kv4.2 potassium channels in the methylazoxymethanol model of cortical malformations and epilepsy.
2001,
Pubmed
Chen,
Dipeptidyl peptidase IV gene family. The DPIV family.
2003,
Pubmed
Cheng,
DREAM is a critical transcriptional repressor for pain modulation.
2002,
Pubmed
Connor,
Prediction of repetitive firing behaviour from voltage clamp data on an isolated neurone soma.
1971,
Pubmed
Dong,
Dopamine D1-class receptors selectively modulate a slowly inactivating potassium current in rat medial prefrontal cortex pyramidal neurons.
2003,
Pubmed
Gebauer,
N-type inactivation features of Kv4.2 channel gating.
2004,
Pubmed
Guo,
Role of heteromultimers in the generation of myocardial transient outward K+ currents.
2002,
Pubmed
Hoffman,
K+ channel regulation of signal propagation in dendrites of hippocampal pyramidal neurons.
1997,
Pubmed
Holmqvist,
Elimination of fast inactivation in Kv4 A-type potassium channels by an auxiliary subunit domain.
2002,
Pubmed
,
Xenbase
Hough,
Rump white inversion in the mouse disrupts dipeptidyl aminopeptidase-like protein 6 and causes dysregulation of Kit expression.
1998,
Pubmed
Huguenard,
A fast transient potassium current in thalamic relay neurons: kinetics of activation and inactivation.
1991,
Pubmed
Jerng,
Modulation of Kv4.2 channel expression and gating by dipeptidyl peptidase 10 (DPP10).
2004,
Pubmed
,
Xenbase
Jerng,
K+ channel inactivation mediated by the concerted action of the cytoplasmic N- and C-terminal domains.
1997,
Pubmed
,
Xenbase
Jerng,
Inactivation gating of Kv4 potassium channels: molecular interactions involving the inner vestibule of the pore.
1999,
Pubmed
,
Xenbase
Jerng,
Molecular physiology and modulation of somatodendritic A-type potassium channels.
2004,
Pubmed
Johns,
Suppression of neuronal and cardiac transient outward currents by viral gene transfer of dominant-negative Kv4.2 constructs.
1997,
Pubmed
Johnston,
Dendritic potassium channels in hippocampal pyramidal neurons.
2000,
Pubmed
Kin,
Biosynthesis and characterization of the brain-specific membrane protein DPPX, a dipeptidyl peptidase IV-related protein.
2001,
Pubmed
Klee,
Comparison of voltage-dependent potassium currents in rat pyramidal neurons acutely isolated from hippocampal regions CA1 and CA3.
1995,
Pubmed
Klemic,
Inactivation of Kv2.1 potassium channels.
1998,
Pubmed
,
Xenbase
Korngreen,
Voltage-gated K+ channels in layer 5 neocortical pyramidal neurones from young rats: subtypes and gradients.
2000,
Pubmed
Kunjilwar,
KChIP3 rescues the functional expression of Shal channel tetramerization mutants.
2004,
Pubmed
Lien,
Gating, modulation and subunit composition of voltage-gated K(+) channels in dendritic inhibitory interneurones of rat hippocampus.
2002,
Pubmed
Lilliehook,
Altered Abeta formation and long-term potentiation in a calsenilin knock-out.
2003,
Pubmed
Malin,
Elimination of the fast transient in superior cervical ganglion neurons with expression of KV4.2W362F: molecular dissection of IA.
2000,
Pubmed
Martina,
Functional and molecular differences between voltage-gated K+ channels of fast-spiking interneurons and pyramidal neurons of rat hippocampus.
1998,
Pubmed
Nadal,
The CD26-related dipeptidyl aminopeptidase-like protein DPPX is a critical component of neuronal A-type K+ channels.
2003,
Pubmed
,
Xenbase
Nagase,
Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.
2000,
Pubmed
Nagase,
Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro.
2000,
Pubmed
Petersen,
Expression environment determines K+ current properties: Kv1 and Kv4 alpha-subunit-induced K+ currents in mammalian cell lines and cardiac myocytes.
1999,
Pubmed
,
Xenbase
Qi,
Cloning and characterization of dipeptidyl peptidase 10, a new member of an emerging subgroup of serine proteases.
2003,
Pubmed
Rhodes,
KChIPs and Kv4 alpha subunits as integral components of A-type potassium channels in mammalian brain.
2004,
Pubmed
Rüschenschmidt,
Characterization of a fast transient outward current in neocortical neurons from epilepsy patients.
2004,
Pubmed
Schoppa,
Regulation of synaptic timing in the olfactory bulb by an A-type potassium current.
1999,
Pubmed
Schrader,
PKA modulation of Kv4.2-encoded A-type potassium channels requires formation of a supramolecular complex.
2002,
Pubmed
,
Xenbase
Serôdio,
Differential expression of Kv4 K+ channel subunits mediating subthreshold transient K+ (A-type) currents in rat brain.
1998,
Pubmed
Shibata,
A-type K+ current mediated by the Kv4 channel regulates the generation of action potential in developing cerebellar granule cells.
2000,
Pubmed
Spreafico,
Mouse DREAM/calsenilin/KChIP3: gene structure, coding potential, and expression.
2001,
Pubmed
Strop,
Structure of a human A-type potassium channel interacting protein DPPX, a member of the dipeptidyl aminopeptidase family.
2004,
Pubmed
Tkatch,
Kv4.2 mRNA abundance and A-type K(+) current amplitude are linearly related in basal ganglia and basal forebrain neurons.
2000,
Pubmed
Van Hoorick,
Differential modulation of Kv4 kinetics by KCHIP1 splice variants.
2003,
Pubmed
Wada,
Differential expression of two distinct forms of mRNA encoding members of a dipeptidyl aminopeptidase family.
1992,
Pubmed
Watanabe,
Dendritic K+ channels contribute to spike-timing dependent long-term potentiation in hippocampal pyramidal neurons.
2002,
Pubmed
Xiong,
Differential distribution of KChIPs mRNAs in adult mouse brain.
2004,
Pubmed
Zagha,
DPP10 modulates Kv4-mediated A-type potassium channels.
2005,
Pubmed
Zaidi,
Biochemical and immunocytochemical characterization of calsenilin in mouse brain.
2002,
Pubmed
Zhou,
Structural insights into the functional interaction of KChIP1 with Shal-type K(+) channels.
2004,
Pubmed
de Lecea,
Transcripts encoding a neural membrane CD26 peptidase-like protein are stimulated by synaptic activity.
1994,
Pubmed
