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Mol Biol Cell
1998 Jun 01;96:1293-308. doi: 10.1091/mbc.9.6.1293.
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The UNI3 gene is required for assembly of basal bodies of Chlamydomonas and encodes delta-tubulin, a new member of the tubulin superfamily.
Dutcher SK
,
Trabuco EC
.
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We have cloned the UNI3 gene in Chlamydomonas and find that it encodes a new member of the tubulin superfamily. Although Uni3p shares significant sequence identity with alpha-, beta-, and gamma-tubulins, there is a region of Uni3p that has no similarity to tubulins or other known proteins. Mutant uni3-1 cells assemble zero, one, or two flagella. Pedigree analysis suggests that flagellar number in uni3-1 cells is a function of the age of the cell. The uniflagellate uni3-1 cells show a positional phenotype; the basal body opposite the eyespot templates the single flagellum. A percentage of uni3-1 cells also fail to orient the cleavage furrow properly, and basal bodies have been implicated in the placement of cleavage furrows in Chlamydomonas. Finally when uni3-1 cells are observed by electron microscopy, doublet rather than triplet microtubules are observed at the proximal end of the basal bodies. We propose that the Uni3 tubulin is involved in both the function and cell cycle-dependent maturation of basal bodies/centrioles.
Altschul,
Basic local alignment search tool.
1990, Pubmed
Altschul,
Basic local alignment search tool.
1990,
Pubmed
Bairoch,
The PROSITE dictionary of sites and patterns in proteins, its current status.
1993,
Pubmed
Burns,
Identification of two new members of the tubulin family.
1995,
Pubmed
Callaini,
Centriole and centrosome dynamics during the embryonic cell cycles that follow the formation of the cellular blastoderm in Drosophila.
1997,
Pubmed
Debuchy,
The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus.
1989,
Pubmed
Devereux,
A comprehensive set of sequence analysis programs for the VAX.
1984,
Pubmed
Dutcher,
Genetic properties of linkage group XIX in Chlamydomonas reinhardtii.
1986,
Pubmed
Dutcher,
Mating and tetrad analysis in Chlamydomonas reinhardtii.
1995,
Pubmed
Ehler,
Loss of spatial control of the mitotic spindle apparatus in a Chlamydomonas reinhardtii mutant strain lacking basal bodies.
1995,
Pubmed
Felsenstein,
Inferring phylogenies from protein sequences by parsimony, distance, and likelihood methods.
1996,
Pubmed
Fernández,
Isolation and characterization of the nitrate reductase structural gene of Chlamydomonas reinhardtii.
1989,
Pubmed
Ferris,
The mating-type locus of Chlamydomonas reinhardtii contains highly rearranged DNA sequences.
1994,
Pubmed
Fitch,
Construction of phylogenetic trees.
1967,
Pubmed
Foster,
Light Antennas in phototactic algae.
1980,
Pubmed
Félix,
Centrosome assembly in vitro: role of gamma-tubulin recruitment in Xenopus sperm aster formation.
1994,
Pubmed
,
Xenbase
Goodenough,
Interrelationships between microtubules, a striated fiber, and the gametic mating structure of Chlamydomonas reinhardi.
1978,
Pubmed
Gumpel,
Studies on homologous recombination in the green alga Chlamydomonas reinhardtii.
1994,
Pubmed
Henikoff,
Protein family classification based on searching a database of blocks.
1994,
Pubmed
Holmes,
Linkage group XIX of Chlamydomonas reinhardtii has a linear map.
1993,
Pubmed
Holmes,
Cellular asymmetry in Chlamydomonas reinhardtii.
1989,
Pubmed
Horio,
The fission yeast gamma-tubulin is essential for mitosis and is localized at microtubule organizing centers.
1991,
Pubmed
Horst,
ptx1, a nonphototactic mutant of Chlamydomonas, lacks control of flagellar dominance.
1993,
Pubmed
Huang,
Uniflagellar mutants of Chlamydomonas: evidence for the role of basal bodies in transmission of positional information.
1982,
Pubmed
Huffaker,
Genetic analysis of the yeast cytoskeleton.
1987,
Pubmed
James,
A mutation in the alpha 1-tubulin gene of Chlamydomonas reinhardtii confers resistance to anti-microtubule herbicides.
1993,
Pubmed
Johnson,
Molecular studies of linkage group XIX of Chlamydomonas reinhardtii: evidence against a basal body location.
1991,
Pubmed
Johnson,
Fine structure of cell division in Chlamydomonas reinhardi. Basal bodies and microtubules.
1968,
Pubmed
Keeling,
Highly divergent Caenorhabditis and Saccharomyces tubulins evolved recently from genes encoding gamma-tubulin.
1996,
Pubmed
Keeling,
Alpha-tubulin from early-diverging eukaryotic lineages and the evolution of the tubulin family.
1996,
Pubmed
Kindle,
High-frequency nuclear transformation of Chlamydomonas reinhardtii.
1990,
Pubmed
King,
Phosphoregulation of an inner dynein arm complex in Chlamydomonas reinhardtii is altered in phototactic mutant strains.
1997,
Pubmed
Kochanski,
Mode of centriole duplication and distribution.
1990,
Pubmed
Lange,
A molecular marker for centriole maturation in the mammalian cell cycle.
1995,
Pubmed
LeDizet,
ida4-1, ida4-2, and ida4-3 are intron splicing mutations affecting the locus encoding p28, a light chain of Chlamydomonas axonemal inner dynein arms.
1995,
Pubmed
Lux,
Genetic interactions at the FLA10 locus: suppressors and synthetic phenotypes that affect the cell cycle and flagellar function in Chlamydomonas reinhardtii.
1991,
Pubmed
Mahowald,
Intercellular migration of centrioles in the germarium of Drosophila melanogaster. An electron microscopic study.
1970,
Pubmed
Marschall,
Analysis of Tub4p, a yeast gamma-tubulin-like protein: implications for microtubule-organizing center function.
1996,
Pubmed
McDonald,
Improved preservation of ultrastructure in difficult-to-fix organisms by high pressure freezing and freeze substitution: I. Drosophila melanogaster and Strongylocentrotus purpuratus embryos.
1993,
Pubmed
Mello,
The PIE-1 protein and germline specification in C. elegans embryos.
1996,
Pubmed
Miklos,
An essential cell division gene of Drosophila, absent from Saccharomyces, encodes an unusual protein with tubulin-like and myosin-like peptide motifs.
1997,
Pubmed
Moritz,
Three-dimensional structural characterization of centrosomes from early Drosophila embryos.
1995,
Pubmed
Myster,
The Chlamydomonas Dhc1 gene encodes a dynein heavy chain subunit required for assembly of the I1 inner arm complex.
1997,
Pubmed
Nelson,
Targeted disruption of the NIT8 gene in Chlamydomonas reinhardtii.
1995,
Pubmed
Oakley,
Identification of gamma-tubulin, a new member of the tubulin superfamily encoded by mipA gene of Aspergillus nidulans.
1989,
Pubmed
Oakley,
Gamma-tubulin is a component of the spindle pole body that is essential for microtubule function in Aspergillus nidulans.
1990,
Pubmed
Paintrand,
Centrosome organization and centriole architecture: their sensitivity to divalent cations.
1992,
Pubmed
Pazour,
Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii.
1995,
Pubmed
Pearson,
Improved tools for biological sequence comparison.
1988,
Pubmed
Porter,
Extragenic suppressors of paralyzed flagellar mutations in Chlamydomonas reinhardtii identify loci that alter the inner dynein arms.
1992,
Pubmed
Ranum,
Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms.
1988,
Pubmed
Ringo,
Flagellar motion and fine structure of the flagellar apparatus in Chlamydomonas.
1967,
Pubmed
Smith,
PF16 encodes a protein with armadillo repeats and localizes to a single microtubule of the central apparatus in Chlamydomonas flagella.
1996,
Pubmed
Sobel,
A highly divergent gamma-tubulin gene is essential for cell growth and proper microtubule organization in Saccharomyces cerevisiae.
1995,
Pubmed
Sonnhammer,
Pfam: a comprehensive database of protein domain families based on seed alignments.
1997,
Pubmed
Spang,
gamma-Tubulin-like Tub4p of Saccharomyces cerevisiae is associated with the spindle pole body substructures that organize microtubules and is required for mitotic spindle formation.
1996,
Pubmed
,
Xenbase
Stearns,
Gamma-tubulin is a highly conserved component of the centrosome.
1991,
Pubmed
,
Xenbase
Stearns,
In vitro reconstitution of centrosome assembly and function: the central role of gamma-tubulin.
1994,
Pubmed
,
Xenbase
Sulston,
The C. elegans genome sequencing project: a beginning.
1992,
Pubmed
Sunkel,
Gamma-tubulin is required for the structure and function of the microtubule organizing centre in Drosophila neuroblasts.
1995,
Pubmed
Tam,
Cloning of flagellar genes in Chlamydomonas reinhardtii by DNA insertional mutagenesis.
1993,
Pubmed
Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed
Vassilev,
Identification of intrinsic dimer and overexpressed monomeric forms of gamma-tubulin in Sf9 cells infected with baculovirus containing the Chlamydomonas gamma-tubulin sequence.
1995,
Pubmed
Vorobjev,
Centrioles in the cell cycle. I. Epithelial cells.
1982,
Pubmed
Youngblom,
The two beta-tubulin genes of Chlamydomonas reinhardtii code for identical proteins.
1984,
Pubmed
Zheng,
Nucleation of microtubule assembly by a gamma-tubulin-containing ring complex.
1995,
Pubmed
,
Xenbase
