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Dev Biol 1997 Oct 01;1901:1-17. doi: 10.1006/dbio.1997.8692.
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xnf7 functions in dorsal-ventral patterning of the Xenopus embryo.

El-Hodiri HM , Shou W , Etkin LD .

Xenopus nuclear factor 7 (xnf7) is a maternally expressed nuclear protein that is retained in the cytoplasm from oocyte maturation until the midblastula transition (MBT). Mutations of the xnf7 phosphorylation sites to glutamic acids (dnxnf7) resulted in the retention of the endogenous protein in the cytoplasm past the MBT, indicating that cytoplasmic retention is a phosphorylation dependent process. In addition, dnxnf7 acted as a dominant negative mutant by keeping the endogenous xnf7 protein in the cytoplasm past the MBT. Overexpression of dnxnf7 in future dorsal blastomeres resulted in a ventralized or posteriorized phenotype in which the embryos lacked anterior structures, while overexpression in ventral blastomeres resulted in dorsalized embryos. dnxnf7 also affected the expression of both dorsal and ventral mesodermal markers. These data suggest that xnf7 functions in dorsal/ventral patterning and that the movement of the protein from the cytoplasm to the nucleus at the MBT is critical for the execution of a genetic program conferring a dorsal or ventral identity to the mesoderm.

PubMed ID: 9331327
Article link: Dev Biol

Species referenced: Xenopus
Genes referenced: chrd.1 mrpl24 tbx2 tbxt ventx1 ventx1.2 xnf7
Antibodies: Xnf7 Ab3

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