Mukhopadhyay A , Barbieri AM , Funato K , Roberts R , Stahl PD .

	Figure 2. Effect of different Rab proteins on HRP uptake in oocytes. Oocytes were injected with 100 ng of the indicated Rabs in 50 nl of microinjection buffer and incubated for 2 h at 18°C. The cells were then incubated with HRP (2 mg/ml) for 1 h at 18°C to determine the uptake rate. Results were expressed as percentage of uptake per oocyte with respect to control (220 ng per oocyte) ± SD (average of eight experiments).
	Figure 3. Effect of Rab5 and its mutants on endocytosis in oocytes. (a) Oocytes were injected with 100 ng each of the indicated proteins, and the uptake was carried out as described in Fig. 1. The results were expressed as percentage of control (200 ng/oocyte) ± SD. (b) Oocytes were injected with 100 ng of Rab5:Q79L or Rab5:ΔC4 in 50 nl of microinjection buffer and incubated for 2 h at 18°C. The cells were lysed, and crude membrane and cytosolic fractions were prepared by centrifuging at 70,000 rpm for 15 min. Each fraction (5 μg) was separated by SDS-PAGE and transferred onto nitrocellulose, and Rab5 protein was localized using polyclonal Rab5-specific antibodies (Alvarez-Dominguez et al., 1996).
	Figure 4. Inhibition of HRP uptake by anti-Rab5 antibody. The oocytes were injected with Rab5:Q79L (100 ng) alone or with anti-Rab5 antibody (200 ng). HRP uptake was followed as described in Fig. 1. Results are expressed as percentage of control uptake (150 ng per oocyte) from the average of eight experiments.
	Figure 5. Effect of NSF on HRP uptake by oocytes. (a) Cells were injected with either 100 ng of Rab5:Q79L with or without anti-NSF (200 ng) antibody. Uptake was carried out as described in Materials and Methods. Results were expressed as percentage of control uptake (180 ng/oocyte) from the average of eight experiments. (b) Oocytes were injected with 75 ng of Rab5:Q79L alone or with 100 ng of NSF and its mutants as indicated. Uptake was determined as described, and the results are expressed as percentage of control (220 ng per oocyte) uptake.
	Figure 6. Rab7-mediated uptake of HRP by oocytes. Oocytes were injected with 100 ng of the indicated proteins and uptake was carried out with HRP-FITC for 1 h in the dark (1 mg/ml). Subsequently, the cells were washed three times with MBS, incubated in the dark for another 1 h, and then observed under the confocal microscope.
	Figure 7. Rab7-mediated inactivation/degradation of HRP by oocytes. (a) Oocytes were preloaded with HRP by incubating with HRP (2 mg/ml) for 1 h at 18°C. Preloaded cells were washed and incubated with 100 μM monensin or control buffer as indicated for 1 h at 18°C. The cells were then injected with 100 ng of the respective fusion proteins. HRP activity present in the cells was measured after a 2-h incubation at 18°C. Results are expressed as percentage of control (110 ng HRP/oocyte) from the average of eight experiments. (b) Oocytes were incubated with 125I-HRP (0.5 mg/ml; 2 × 105 cpm/μg) for 1 h at 18°C and washed three times to remove unbound radioactivity. Subsequently, the cells were injected with 100 ng of Rab7:WT protein or control buffer, and nine oocytes were incubated in 300 μl of MBS for 2 h at 18°C. Oocytes were washed and solublized in PBS containing 0.1% Triton X-100, and TCA-soluble and -precipitable radioactivity was determined in cell lysates and media. Results are expressed as ng of HRP associated with or degraded by the cells.
	Figure 8. Rab5 regulation of endocytosis is upstream of Rab7. (a) Rab5 proteins were injected alone (50 ng) or in combination with both mutants (100 ng) of Rab5 and Rab7. (b) Rab7 proteins (50 ng) were injected alone or in combination with both mutants (100 ng) of Rab5 and Rab7. Results are expressed as percentage of control uptake by uninjected oocytes (average of eight experiments).
	Figure 9. Rab5- and Rab7-coinjected cells produced additive effects. Oocytes were injected with 50 ng of Rab5 and Rab7 alone or in combination and incubated for 2 h at 18°C. The cells were incubated with 2 mg/ml of HRP for 1 h at 18°C to determine the rate of HRP uptake. Results are expressed as percentage of uptake per oocyte with respect to control (205 ng per oocyte) ±SD (average of eight experiments).

Alvarez-Dominguez, Phagocytosed live Listeria monocytogenes influences Rab5-regulated in vitro phagosome-endosome fusion. 1996, Pubmed

Alvarez-Dominguez, Phagocytosed live Listeria monocytogenes influences Rab5-regulated in vitro phagosome-endosome fusion. 1996, Pubmed
Balch, Small GTP-binding proteins in vesicular transport. 1990, Pubmed
Bar-Sagi, Induction of membrane ruffling and fluid-phase pinocytosis in quiescent fibroblasts by ras proteins. 1986, Pubmed
Barbieri, Rab5, an early acting endosomal GTPase, supports in vitro endosome fusion without GTP hydrolysis. 1994, Pubmed
Barbieri, Characterization of Rab5:Q79L-stimulated endosome fusion. 1996, Pubmed
Barr, Trimeric G proteins and vesicle formation. 1992, Pubmed
Beckers, Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein. 1989, Pubmed
Berón, Membrane trafficking along the phagocytic pathway. 1995, Pubmed
Bourne, Do GTPases direct membrane traffic in secretion? 1988, Pubmed
Bourne, The GTPase superfamily: a conserved switch for diverse cell functions. 1990, Pubmed
Bucci, The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway. 1992, Pubmed
Burgoyne, Trimeric G proteins in Golgi transport. 1992, Pubmed
Burstein, Amino acid residues in the Ras-like GTPase Rab3A that specify sensitivity to factors that regulate the GTP/GDP cycling of Rab3A. 1992, Pubmed
Busson, Pathway and kinetics of vitellogenin-gold internalization in the Xenopus oocyte. 1989, Pubmed , Xenbase
Chabre, Aluminofluoride and beryllofluoride complexes: a new phosphate analogs in enzymology. 1990, Pubmed
Chavrier, Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments. 1990, Pubmed
Colombo, Characterization of trypsin-sensitive factor(s) required for endosome-endosome fusion. 1991, Pubmed
Colombo, Inhibition of endocytic transport by aluminum fluoride implicates GTPases as regulators of endocytosis. 1994, Pubmed
Colombo, Evidence of a role for heterotrimeric GTP-binding proteins in endosome fusion. 1992, Pubmed
Colombo, A possible predocking attachment site for N-ethylmaleimide-sensitive fusion protein. Insights from in vitro endosome fusion. 1996, Pubmed
Cresswell, Assembly, transport, and function of MHC class II molecules. 1994, Pubmed
Damke, Clathrin-independent pinocytosis is induced in cells overexpressing a temperature-sensitive mutant of dynamin. 1995, Pubmed
Desjardins, Molecular characterization of phagosomes. 1994, Pubmed
Diaz, In vitro fusion of endosomes following receptor-mediated endocytosis. 1988, Pubmed
Diaz, Vesicle fusion following receptor-mediated endocytosis requires a protein active in Golgi transport. 1989, Pubmed
Feig, Inhibition of NIH 3T3 cell proliferation by a mutant ras protein with preferential affinity for GDP. 1988, Pubmed
Feng, Rab 7: an important regulator of late endocytic membrane traffic. 1995, Pubmed
Germain, MHC-dependent antigen processing and peptide presentation: providing ligands for T lymphocyte activation. 1994, Pubmed
Goda, Selective recycling of the mannose 6-phosphate/IGF-II receptor to the trans Golgi network in vitro. 1988, Pubmed
Goldstein, Receptor-mediated endocytosis: concepts emerging from the LDL receptor system. 1985, Pubmed
Gorvel, rab5 controls early endosome fusion in vitro. 1991, Pubmed
Goud, Small GTP-binding protein associated with Golgi cisternae. 1990, Pubmed
Gravotta, Transport of influenza HA from the trans-Golgi network to the apical surface of MDCK cells permeabilized in their basolateral plasma membranes: energy dependence and involvement of GTP-binding proteins. 1990, Pubmed
Griffiths, Characterization of the cation-independent mannose 6-phosphate receptor-enriched prelysosomal compartment in NRK cells. 1990, Pubmed
Haas, The GTPase Ypt7p of Saccharomyces cerevisiae is required on both partner vacuoles for the homotypic fusion step of vacuole inheritance. 1995, Pubmed
Hwang, Inhibition of SDC25 C-domain-induced guanine-nucleotide exchange by guanine ring binding domain mutants of v-H-ras. 1993, Pubmed
Li, Structure-function relationship of the small GTPase rab5. 1993, Pubmed
Li, Structural features of the GTP-binding defective Rab5 mutants required for their inhibitory activity on endocytosis. 1994, Pubmed
Li, Evidence for phosphatidylinositol 3-kinase as a regulator of endocytosis via activation of Rab5. 1995, Pubmed
Lombardi, Rab9 functions in transport between late endosomes and the trans Golgi network. 1993, Pubmed
Ludwig, Distribution of newly synthesized lysosomal enzymes in the endocytic pathway of normal rat kidney cells. 1991, Pubmed
Mayer, Sec18p (NSF)-driven release of Sec17p (alpha-SNAP) can precede docking and fusion of yeast vacuoles. 1996, Pubmed
Mayorga, Regulatory role for GTP-binding proteins in endocytosis. 1989, Pubmed
Mayorga, Inhibition of endosome fusion by phospholipase A2 (PLA2) inhibitors points to a role for PLA2 in endocytosis. 1993, Pubmed
Mayorga, GTP gamma S stimulation of endosome fusion suggests a role for a GTP-binding protein in the priming of vesicles before fusion. 1989, Pubmed
Mittal, Formation of a transition-state analog of the Ras GTPase reaction by Ras-GDP, tetrafluoroaluminate, and GTPase-activating proteins. 1996, Pubmed
Mullock, Lysosomes can fuse with a late endosomal compartment in a cell-free system from rat liver. 1994, Pubmed
Méresse, The rab7 GTPase resides on a vesicular compartment connected to lysosomes. 1995, Pubmed
Nuoffer, A GDP-bound of rab1 inhibits protein export from the endoplasmic reticulum and transport between Golgi compartments. 1994, Pubmed
Olkkonen, Molecular cloning and subcellular localization of three GTP-binding proteins of the rab subfamily. 1993, Pubmed
Opresko, Differential postendocytotic compartmentation in Xenopus oocytes is mediated by a specifically bound ligand. 1980, Pubmed , Xenbase
Opresko, Specific proteolysis regulates fusion between endocytic compartments in Xenopus oocytes. 1987, Pubmed , Xenbase
Pfeffer, Rab GTPases: master regulators of membrane trafficking. 1994, Pubmed
Qiu, Separation of subcellular compartments containing distinct functional forms of MHC class II. 1994, Pubmed
Rabinowitz, Immunocytochemical characterization of the endocytic and phagolysosomal compartments in peritoneal macrophages. 1992, Pubmed
Riederer, Lysosome biogenesis requires Rab9 function and receptor recycling from endosomes to the trans-Golgi network. 1994, Pubmed
Rothman, Mechanisms of intracellular protein transport. 1994, Pubmed
Schimmöller, Involvement of Ypt7p, a small GTPase, in traffic from late endosome to the vacuole in yeast. 1993, Pubmed
Schweighoffer, The Saccharomyces cerevisiae SDC25 C-domain gene product overcomes the dominant inhibitory activity of Ha-Ras Asn-17. 1993, Pubmed
Singer-Krüger, Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast. 1994, Pubmed
Stenmark, Inhibition of rab5 GTPase activity stimulates membrane fusion in endocytosis. 1994, Pubmed
Swick, Promoter-cDNA-directed heterologous protein expression in Xenopus laevis oocytes. 1992, Pubmed , Xenbase
Sztul, Control of protein traffic between distinct plasma membrane domains. Requirement for a novel 108,000 protein in the fusion of transcytotic vesicles with the apical plasma membrane. 1993, Pubmed
Söllner, SNAP receptors implicated in vesicle targeting and fusion. 1993, Pubmed
Søgaard, A rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles. 1994, Pubmed
Tisdale, GTP-binding mutants of rab1 and rab2 are potent inhibitors of vesicular transport from the endoplasmic reticulum to the Golgi complex. 1992, Pubmed
Tuomikoski, Inhibition of endocytic vesicle fusion in vitro by the cell-cycle control protein kinase cdc2. , Pubmed , Xenbase
Van Der Sluijs, The small GTP-binding protein rab4 is associated with early endosomes. 1991, Pubmed
Wall, Multivesicular bodies play a key role in vitellogenin endocytosis by Xenopus oocytes. 1987, Pubmed , Xenbase
Whiteheart, N-ethylmaleimide-sensitive fusion protein: a trimeric ATPase whose hydrolysis of ATP is required for membrane fusion. 1994, Pubmed
Wichmann, Endocytosis in yeast: evidence for the involvement of a small GTP-binding protein (Ypt7p). 1992, Pubmed
Wilson, A multisubunit particle implicated in membrane fusion. 1992, Pubmed
Wolters, Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen. 1976, Pubmed
Yamashiro, Segregation of transferrin to a mildly acidic (pH 6.5) para-Golgi compartment in the recycling pathway. 1984, Pubmed
Zerial, Rab GTPases in vesicular transport. 1993, Pubmed