Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Cytoplasmic regulation of the length of poly(A) on mRNA is a well-characterized process involved in translational control during development. In contrast, there is no direct in vivo evidence for regulation of the length of poly(A) added during nuclear pre-mRNA processing in somatic cells. We previously reported that Xenopus serum albumin [Schoenberg et al. (1989) Mol. Endocrinol. 3, 805-815] and transferrin [Pastori et al. (1992) J. Steroid Biochem. Mol. Biol. 42, 649-657], mRNA have exceptionally short poly(A) tails ranging from 12 to 17 residues, whereas vitellogenin mRNA has long poly(A). An RT-PCR protocol was adapted to determine the length of poly(A) added onto pre-mRNA, defined here as that species bearing the terminal intron. Using this assay we show that vitellogenin pre-mRNA has the same long poly(A) tail as mature vitellogenin mRNA. In contrast, albumin pre-mRNA has the same short poly(A) as found on fully-processed albumin mRNA. These results indicate that the short poly(A) tail on albumin mRNA results from regulation of poly(A) addition during nuclear 3' processing.
Baurén,
Splicing of Balbiani ring 1 gene pre-mRNA occurs simultaneously with transcription.
1994, Pubmed
Baurén,
Splicing of Balbiani ring 1 gene pre-mRNA occurs simultaneously with transcription.
1994,
Pubmed
Bienroth,
Assembly of a processive messenger RNA polyadenylation complex.
1993,
Pubmed
Carrazana,
The vasopressin mRNA poly(A) tract is unusually long and increases during stimulation of vasopressin gene expression in vivo.
1988,
Pubmed
Decker,
A turnover pathway for both stable and unstable mRNAs in yeast: evidence for a requirement for deadenylation.
1993,
Pubmed
Dompenciel,
Purification and characterization of an estrogen-regulated Xenopus liver polysomal nuclease involved in the selective destabilization of albumin mRNA.
1995,
Pubmed
,
Xenbase
Greenberg,
Deadenylylation: a mechanism controlling c-fos mRNA decay.
1990,
Pubmed
Huarte,
Transient translational silencing by reversible mRNA deadenylation.
1992,
Pubmed
Krizman,
Efficient selection of 3'-terminal exons from vertebrate DNA.
1993,
Pubmed
MacDonald,
The 64-kilodalton subunit of the CstF polyadenylation factor binds to pre-mRNAs downstream of the cleavage site and influences cleavage site location.
1994,
Pubmed
Manley,
A complex protein assembly catalyzes polyadenylation of mRNA precursors.
1995,
Pubmed
Moskaitis,
Xenopus laevis serum albumin: sequence of the complementary deoxyribonucleic acids encoding the 68- and 74-kilodalton peptides and the regulation of albumin gene expression by thyroid hormone during development.
1989,
Pubmed
,
Xenbase
Muhlrad,
Deadenylation of the unstable mRNA encoded by the yeast MFA2 gene leads to decapping followed by 5'-->3' digestion of the transcript.
1994,
Pubmed
Murthy,
Characterization of the multisubunit cleavage-polyadenylation specificity factor from calf thymus.
1992,
Pubmed
Nesic,
Lack of an effect of the efficiency of RNA 3'-end formation on the efficiency of removal of either the final or the penultimate intron in intact cells.
1995,
Pubmed
Pastori,
Coordinate estrogen-regulated instability of serum protein-coding messenger RNAs in Xenopus laevis.
1991,
Pubmed
,
Xenbase
Pastori,
Estrogen-induced ribonuclease activity in Xenopus liver.
1991,
Pubmed
,
Xenbase
Pastori,
Differential regulation and polyadenylation of transferrin mRNA in Xenopus liver and oviduct.
1992,
Pubmed
,
Xenbase
Riegel,
Transcriptional and post-transcriptional inhibition of albumin gene expression by estrogen in Xenopus liver.
1986,
Pubmed
,
Xenbase
Sachs,
Poly(A) tail metabolism and function in eucaryotes.
1993,
Pubmed
Sachs,
Nuclear polyadenylate-binding protein.
1985,
Pubmed
Sallés,
Rapid and sensitive analysis of mRNA polyadenylation states by PCR.
1995,
Pubmed
,
Xenbase
Schoenberg,
Extranuclear estrogen-regulated destabilization of Xenopus laevis serum albumin mRNA.
1989,
Pubmed
,
Xenbase
Sheets,
Two phases in the addition of a poly(A) tail.
1989,
Pubmed
Sheflin,
Assessment of transcript polyadenylation by 3' RACE: the response of epidermal growth factor messenger ribonucleic acid to thyroid hormone in the thyroid and submaxillary glands.
1995,
Pubmed
Shyu,
Two distinct destabilizing elements in the c-fos message trigger deadenylation as a first step in rapid mRNA decay.
1991,
Pubmed
Takagaki,
The human 64-kDa polyadenylylation factor contains a ribonucleoprotein-type RNA binding domain and unusual auxiliary motifs.
1992,
Pubmed
Varnum,
Deadenylation of maternal mRNAs during Xenopus oocyte maturation does not require specific cis-sequences: a default mechanism for translational control.
1990,
Pubmed
,
Xenbase
Wahle,
A novel poly(A)-binding protein acts as a specificity factor in the second phase of messenger RNA polyadenylation.
1991,
Pubmed
Wahle,
The biochemistry of 3'-end cleavage and polyadenylation of messenger RNA precursors.
1992,
Pubmed
Wahle,
Poly(A) tail length control is caused by termination of processive synthesis.
1995,
Pubmed
Wilson,
Removal of poly(A) and consequent degradation of c-fos mRNA facilitated by 3' AU-rich sequences.
1988,
Pubmed
Wormington,
Overexpression of poly(A) binding protein prevents maturation-specific deadenylation and translational inactivation in Xenopus oocytes.
1996,
Pubmed
,
Xenbase
Zingg,
Regulation of poly(A) tail size of vasopressin mRNA.
1988,
Pubmed
