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Proc Natl Acad Sci U S A
1996 Oct 15;9321:12014-9. doi: 10.1073/pnas.93.21.12014.
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Depletion of intracellular polyamines relieves inward rectification of potassium channels.
Shyng SL
,
Sha Q
,
Ferrigni T
,
Lopatin AN
,
Nichols CG
.
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Two different approaches were used to examine the in vivo role of polyamines in causing inward rectification of potassium channels. In two-microelectrode voltage-clamp experiments, 24-hr incubation of Xenopus oocytes injected with 50 nl of difluoromethylornithine (5 mM) and methylglyoxal bis(guanylhydrazone) (1 mM) caused an approximate doubling of expressed Kir2.1 currents and relieved rectification by causing an approximately +10-mV shift of the voltage at which currents are half-maximally inhibited. Second, a putrescine auxotrophic, ornithine decarboxylase-deficient Chinese hamster ovary (O-CHO) cell line was stably transfected with the cDNA encoding Kir2.3. Withdrawal of putrescine from the medium led to rapid (1-day) loss of the instantaneous phase of Kir2.3 channel activation, consistent with a decline of intracellular putrescine levels. Four days after putrescine withdrawal, macroscopic conductance, assessed using an 86Rb+ flux assay, was approximately doubled, and this corresponded to a +30-mV shift of V1/2 of rectification. With increasing time after putrescine withdrawal, there was an increase in the slowest phase of current activation, corresponding to an increase in the spermine-to-spermidine ratio over time. These results provide direct evidence for a role of each polyamine in induction of rectification, and they further demonstrate that in vivo modulation of rectification is possible by manipulation of polyamine levels using genetic and pharmacological approaches.
Bartolome,
Role of ornithine decarboxylase in cardiac growth and hypertrophy.
1980, Pubmed
Bartolome,
Role of ornithine decarboxylase in cardiac growth and hypertrophy.
1980,
Pubmed
Basu,
Interaction of a polyamine analogue, 1,19-bis-(ethylamino)-5,10,15- triazanonadecane (BE-4-4-4-4), with DNA and effect on growth, survival, and polyamine levels in seven human brain tumor cell lines.
1993,
Pubmed
Bianchi,
Regulation by spermine of native inward rectifier K+ channels in RBL-1 cells.
1996,
Pubmed
Bowie,
Inward rectification of both AMPA and kainate subtype glutamate receptors generated by polyamine-mediated ion channel block.
1995,
Pubmed
Brismar,
Inward rectifying potassium channels in human malignant glioma cells.
1989,
Pubmed
Caldarera,
Polyamine and nucleic acid metabolism in myocardial hypertrophy of the overloaded heart.
1974,
Pubmed
Constanti,
Fast inward-rectifying current accounts for anomalous rectification in olfactory cortex neurones.
1983,
Pubmed
Donevan,
Intracellular polyamines mediate inward rectification of Ca(2+)-permeable alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors.
1995,
Pubmed
Fakler,
A structural determinant of differential sensitivity of cloned inward rectifier K+ channels to intracellular spermine.
1994,
Pubmed
,
Xenbase
Fakler,
Strong voltage-dependent inward rectification of inward rectifier K+ channels is caused by intracellular spermine.
1995,
Pubmed
,
Xenbase
Feuerstein,
alpha-Difluoromethylornithine alters calcium signaling in platelet-derived growth factor-stimulated A172 brain tumor cells in culture.
1992,
Pubmed
Ficker,
Spermine and spermidine as gating molecules for inward rectifier K+ channels.
1994,
Pubmed
,
Xenbase
Hayashi,
Increases in brain polyamine concentrations in chemical kindling and single convulsion induced by pentylenetetrazol in rats.
1993,
Pubmed
Hestrin,
The properties and function of inward rectification in rod photoreceptors of the tiger salamander.
1987,
Pubmed
Inoue,
Somatostatin induces an inward rectification in rat locus coeruleus neurones through a pertussis toxin-sensitive mechanism.
1988,
Pubmed
Kamboj,
Intracellular spermine confers rectification on rat calcium-permeable AMPA and kainate receptors.
1995,
Pubmed
Kandel,
Anomalous rectification in the metacerebral giant cells and its consequences for synaptic transmission.
1966,
Pubmed
Koh,
Block of native Ca(2+)-permeable AMPA receptors in rat brain by intracellular polyamines generates double rectification.
1995,
Pubmed
Laschet,
Polyamine metabolism in epileptic cortex.
1992,
Pubmed
Lopatin,
Potassium channel block by cytoplasmic polyamines as the mechanism of intrinsic rectification.
1994,
Pubmed
,
Xenbase
Lopatin,
The mechanism of inward rectification of potassium channels: "long-pore plugging" by cytoplasmic polyamines.
1995,
Pubmed
,
Xenbase
Marton,
Polyamines as targets for therapeutic intervention.
1995,
Pubmed
Matsuda,
Triple-barrel structure of inwardly rectifying K+ channels revealed by Cs+ and Rb+ block in guinea-pig heart cells.
1989,
Pubmed
Matsuda,
Ohmic conductance through the inwardly rectifying K channel and blocking by internal Mg2+.
,
Pubmed
Matsuda,
Open-state substructure of inwardly rectifying potassium channels revealed by magnesium block in guinea-pig heart cells.
1988,
Pubmed
Mialon,
Effect of one hyperbaric oxygen-induced convulsion on cortical polyamine content in two strains of mice.
1993,
Pubmed
Newman,
Inward-rectifying potassium channels in retinal glial (Müller) cells.
1993,
Pubmed
Nichols,
Inward rectification and implications for cardiac excitability.
1996,
Pubmed
Osborne,
Polyamine levels during Xenopus laevis oogenesis: a role in oocyte competence to meiotic resumption.
1989,
Pubmed
,
Xenbase
Pegg,
Polyamine metabolism and function.
1982,
Pubmed
Russell,
Amine synthesis in regenerating rat liver: extremely rapid turnover of ornithine decarboxylase.
1969,
Pubmed
Silver,
Effects of external Rb+ on inward rectifier K+ channels of bovine pulmonary artery endothelial cells.
1994,
Pubmed
Standen,
Rubidium block and rubidium permeability of the inward rectifier of frog skeletal muscle fibres.
1980,
Pubmed
Steglich,
An ornithine decarboxylase-deficient mutant of Chinese hamster ovary cells.
1982,
Pubmed
Steglich,
Selection of ornithine decarboxylase-deficient mutants of Chinese hamster ovary cells.
1983,
Pubmed
Tabor,
Polyamines.
1984,
Pubmed
Ulrich,
Function of normal and mutated gamma-globin gene promoters in electroporated K562 erythroleukemia cells.
1990,
Pubmed
Vandenberg,
Inward rectification of a potassium channel in cardiac ventricular cells depends on internal magnesium ions.
1987,
Pubmed
Williams,
Voltage- and ligand-activated inwardly rectifying currents in dorsal raphe neurons in vitro.
1988,
Pubmed
Yamada,
Spermine gates inward-rectifying muscarinic but not ATP-sensitive K+ channels in rabbit atrial myocytes. Intracellular substance-mediated mechanism of inward rectification.
1995,
Pubmed
