Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Philos Trans R Soc Lond B Biol Sci
2005 Mar 29;3601455:591-4. doi: 10.1098/rstb.2004.1613.
Show Gene links
Show Anatomy links
A comparison of the ability of XMAP215 and tau to inhibit the microtubule destabilizing activity of XKCM1.
Noetzel TL
,
Drechsel DN
,
Hyman AA
,
Kinoshita K
.
???displayArticle.abstract???
During mitosis, microtubules not only grow fast, but also have a high rate of catastrophe. This is achieved in part by the activity of the MAP, XMAP215, which can stimulate the growth rate of microtubules without fully inhibiting the function of the catastrophe-kinesin XKCM1. We do not know whether this activity is particular to XMAP215, or is a general property of all MAPs. Here, we compare the activities of XMAP215 with the neuronal MAP tau, in opposing the destabilizing activity of the non-conventional kinesin XKCM1. We show that tau is a much more potent inhibitor of XKCM1 than XMAP215. Because tau completely suppresses XKCM1 activity, even at low concentrations, the combination of tau and XKCM1 is unable to generate mitotic microtubule dynamics.
Belmont,
Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts.
1990, Pubmed,
Xenbase
Belmont,
Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts.
1990,
Pubmed
,
Xenbase
Charrasse,
The TOGp protein is a new human microtubule-associated protein homologous to the Xenopus XMAP215.
1998,
Pubmed
,
Xenbase
Desai,
Kin I kinesins are microtubule-destabilizing enzymes.
1999,
Pubmed
,
Xenbase
Drechsel,
Modulation of the dynamic instability of tubulin assembly by the microtubule-associated protein tau.
1992,
Pubmed
Hirokawa,
Selective stabilization of tau in axons and microtubule-associated protein 2C in cell bodies and dendrites contributes to polarized localization of cytoskeletal proteins in mature neurons.
1996,
Pubmed
Howard,
Dynamics and mechanics of the microtubule plus end.
2003,
Pubmed
Kinoshita,
Reconstitution of physiological microtubule dynamics using purified components.
2001,
Pubmed
,
Xenbase
Kinoshita,
XMAP215: a key component of the dynamic microtubule cytoskeleton.
2002,
Pubmed
,
Xenbase
Lewis,
Microtubule-associated protein MAP2 shares a microtubule binding motif with tau protein.
1988,
Pubmed
Mitchison,
Microtubule assembly nucleated by isolated centrosomes.
,
Pubmed
Mitchison,
Dynamic instability of microtubule growth.
,
Pubmed
Ohkura,
Dis1/TOG universal microtubule adaptors - one MAP for all?
2001,
Pubmed
Tournebize,
Role of chromosomes in assembly of meiotic and mitotic spindles.
1997,
Pubmed
,
Xenbase
Walker,
Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies.
1988,
Pubmed
Walker,
Dilution of individual microtubules observed in real time in vitro: evidence that cap size is small and independent of elongation rate.
1991,
Pubmed
Weingarten,
A protein factor essential for microtubule assembly.
1975,
Pubmed
West,
A model for microtubule-associated protein 4 structure. Domains defined by comparisons of human, mouse, and bovine sequences.
1991,
Pubmed
van Breugel,
Stu2p, the budding yeast member of the conserved Dis1/XMAP215 family of microtubule-associated proteins is a plus end-binding microtubule destabilizer.
2003,
Pubmed
,
Xenbase
