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XB-ART-20793
Neurosci Lett September 26, 1994; 179 (1-2): 100-2.
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Functional coupling of the 5-HT2C serotonin receptor to G proteins in Xenopus oocytes.

Chen Y , Baez M , Yu L .


Abstract
The serotonin 2c (5-HT2C) receptor mediates its cellular effects by interacting with heterotrimeric guanine nucleotide binding proteins (G proteins). To characterize which G proteins are involved in functional coupling to the receptor, a mouse 5-HT2C receptor was expressed in Xenopus oocytes, and antisense oligoncleotides complementary to the mRNA sequence of the endogenous Xenopus G protein alpha subunits were used to inhibit G protein synthesis. Antisense oligonucleotide against the Xenopus G(o) alpha subunit inhibited the 5-HT2C receptor function, and coexpression of a rat G(o) alpha subunit reversed the inhibition by the anti-Xenopus G(o) oligonucleotide. Furthermore, antisense oligonucleotides against both the G(o) and Gi1 alpha subunits inhibited the electrophysiologic response induced by stimulation of the 5-HT2C receptor. These data suggest that both G(o) and Gi1 are involved in functional coupling of the 5-HT2C receptor to phospholipase C in Xenopus oocytes.

PubMed ID: 7531308
Article link: Neurosci Lett
Grant support: [+]

Species referenced: Xenopus
Genes referenced: htr2c