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Kiefer P
,
Mathieu M
,
Close MJ
,
Peters G
,
Dickson C
.
???displayArticle.abstract??? Fibroblast growth factor 3 (FGF3) was first identified as the product of a cellular oncogene activated by mouse mammary tumour virus but its normal role appears to be in the developing embryo. To gain further insights into its function, we have isolated sequences encoding the FGF3 homologue in Xenopus laevis, XFGF3. COS-1 cells transfected with XFGF3 cDNA express a 31 kDa product, p31, generated by signal peptide cleavage and Asn-linked glycosylation at the single consensus site. This product is secreted and becomes associated with the cell surface and extracellular matrix. Proteolytic cleavage of p31 in the extracellular compartment results in an amino-terminally truncated product, p27, that is also glycosylated. Both p31 and p27 bind quantitatively to heparin-Sepharose and can be displaced from the cell surface and extracellular matrix by soluble heparin. Conditioned medium containing these two proteins is capable of inducing transient morphological transformation of NIH3T3 cells and of stimulating DNA synthesis in quiescent C57MG and BALB/MK cells which express different isoforms of FGF receptors 1 and 2. Since XFGF3 behaves very differently from its mouse counterpart, we constructed chimeras in which amino-terminal sequences from XFGF3 were fused with carboxy-terminal sequences from mouse FGF3. Increasing the contribution from mouse FGF3 led to a more restricted host range for the chimeric ligand.
Acland,
Subcellular fate of the int-2 oncoprotein is determined by choice of initiation codon.
1990, Pubmed
Acland,
Subcellular fate of the int-2 oncoprotein is determined by choice of initiation codon.
1990,
Pubmed
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991,
Pubmed
,
Xenbase
Bates,
Biosynthesis of human fibroblast growth factor-5.
1991,
Pubmed
Bottaro,
Characterization of the receptor for keratinocyte growth factor. Evidence for multiple fibroblast growth factor receptors.
1990,
Pubmed
Brookes,
Sequence organization of the human int-2 gene and its expression in teratocarcinoma cells.
1989,
Pubmed
Burgess,
The heparin-binding (fibroblast) growth factor family of proteins.
1989,
Pubmed
Derynck,
Human transforming growth factor-beta complementary DNA sequence and expression in normal and transformed cells.
,
Pubmed
Dickson,
The structure and function of the int-2 oncogene.
1989,
Pubmed
Dixon,
Detection and characterization of the fibroblast growth factor-related oncoprotein INT-2.
1989,
Pubmed
Dorner,
Analysis of synthesis, processing, and secretion of proteins expressed in mammalian cells.
1990,
Pubmed
Finch,
Human KGF is FGF-related with properties of a paracrine effector of epithelial cell growth.
1989,
Pubmed
Friesel,
cDNA cloning and developmental expression of fibroblast growth factor receptors from Xenopus laevis.
1991,
Pubmed
,
Xenbase
Friesel,
Spatially restricted expression of fibroblast growth factor receptor-2 during Xenopus development.
1992,
Pubmed
,
Xenbase
Goldfarb,
Cell transformation by Int-2--a member of the fibroblast growth factor family.
1991,
Pubmed
Goldfarb,
The fibroblast growth factor family.
1990,
Pubmed
Isaacs,
Expression of a novel FGF in the Xenopus embryo. A new candidate inducing factor for mesoderm formation and anteroposterior specification.
1992,
Pubmed
,
Xenbase
Kiefer,
The Int-2/Fgf-3 oncogene product is secreted and associates with extracellular matrix: implications for cell transformation.
1991,
Pubmed
Kimelman,
Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo.
1987,
Pubmed
,
Xenbase
Kimelman,
The presence of fibroblast growth factor in the frog egg: its role as a natural mesoderm inducer.
1988,
Pubmed
,
Xenbase
Kozak,
The scanning model for translation: an update.
1989,
Pubmed
Mansour,
Mice homozygous for a targeted disruption of the proto-oncogene int-2 have developmental defects in the tail and inner ear.
1993,
Pubmed
Miki,
Expression cDNA cloning of the KGF receptor by creation of a transforming autocrine loop.
1991,
Pubmed
Moore,
Sequence, topography and protein coding potential of mouse int-2: a putative oncogene activated by mouse mammary tumour virus.
1986,
Pubmed
Morgenstern,
Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line.
1990,
Pubmed
Muller,
The int-2 gene product acts as an epithelial growth factor in transgenic mice.
1990,
Pubmed
Musci,
Regulation of the fibroblast growth factor receptor in early Xenopus embryos.
1990,
Pubmed
,
Xenbase
Niswander,
Fgf-4 expression during gastrulation, myogenesis, limb and tooth development in the mouse.
1992,
Pubmed
Ornitz,
Int-2, an autocrine and/or ultra-short-range effector in transgenic mammary tissue transplants.
1992,
Pubmed
Pappin,
Solid-phase sequence analysis of proteins electroblotted or spotted onto polyvinylidene difluoride membranes.
1990,
Pubmed
Paterno,
Mesoderm-inducing properties of INT-2 and kFGF: two oncogene-encoded growth factors related to FGF.
1989,
Pubmed
,
Xenbase
Rifkin,
Recent developments in the cell biology of basic fibroblast growth factor.
1989,
Pubmed
Rubin,
Purification and characterization of a newly identified growth factor specific for epithelial cells.
1989,
Pubmed
Ruta,
Receptor for acidic fibroblast growth factor is related to the tyrosine kinase encoded by the fms-like gene (FLG).
1989,
Pubmed
Slack,
Mesoderm induction in early Xenopus embryos by heparin-binding growth factors.
,
Pubmed
,
Xenbase
Smith,
Mesoderm induction and mesoderm-inducing factors in early amphibian development.
1989,
Pubmed
Stamp,
Nonuniform expression of a mouse mammary tumor virus-driven int-2/Fgf-3 transgene in pregnancy-responsive breast tumors.
1992,
Pubmed
Tannahill,
Developmental expression of the Xenopus int-2 (FGF-3) gene: activation by mesodermal and neural induction.
1992,
Pubmed
,
Xenbase
Thompson,
Over-expression of fibroblast growth factors in Xenopus embryos.
1992,
Pubmed
,
Xenbase
Vaidya,
Murine mammary tumor virus (MuMTV) infection of an epithelial cell line established from C57BL/6 mouse mammary glands.
1978,
Pubmed
Venesio,
The int-2 gene product acts as a growth factor and substitutes for basic fibroblast growth factor in promoting the differentiation of a normal mouse mammary epithelial cell line.
1992,
Pubmed
Volk,
An antisense transcript from the Xenopus laevis bFGF gene coding for an evolutionarily conserved 24 kd protein.
1989,
Pubmed
,
Xenbase
Wilkinson,
Expression pattern of the FGF-related proto-oncogene int-2 suggests multiple roles in fetal development.
1989,
Pubmed
Wilkinson,
Expression of the FGF-related proto-oncogene int-2 during gastrulation and neurulation in the mouse.
1988,
Pubmed
von Heijne,
A new method for predicting signal sequence cleavage sites.
1986,
Pubmed
