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EMBO J 1993 Feb 01;122:423-33. doi: 10.1002/j.1460-2075.1993.tb05674.x.
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A nucleosome-dependent static loop potentiates estrogen-regulated transcription from the Xenopus vitellogenin B1 promoter in vitro.

Schild C , Claret FX , Wahli W , Wolffe AP .

We describe the transcriptional potentiation in estrogen responsive transcription extracts of the Xenopus vitellogenin B1 gene promoter through the formation of a positioned nucleosome. Nuclease digestion and hydroxyl radical cleavage indicate that strong, DNA sequence-directed positioning of a nucleosome occurs between -300 and -140 relative to the start site of transcription. Deletion of this DNA sequence abolishes the potentiation of transcription due to nucleosome assembly. The wrapping of DNA around the histone core of the nucleosome positioned between -300 and -140 creates a static loop in which distal estrogen receptor binding sites are brought close to proximal promoter elements. This might facilitate interactions between the trans-acting factors themselves and/or RNA polymerase. Such a nucleosome provides an example of how chromatin structure might have a positive effect on the transcription process.

PubMed ID: 8440235
PMC ID: PMC413225
Article link: EMBO J

Species referenced: Xenopus
Genes referenced: vtgb1

References [+] :
Archer, Transcription factor access is mediated by accurately positioned nucleosomes on the mouse mammary tumor virus promoter. 1991, Pubmed