Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The nuclear pore complex (NPC) is an evolutionarily conserved structure that mediates exchange of macromolecules across the nuclear envelope (NE). It is comprised of approximately 30 proteins termed nucleoporins that are each present in multiple copies. We have investigated the function of the human nucleoporin Nup53, the ortholog of Saccharomyces cerevisiae Nup53p. Both cell fractionation and in vitro binding data suggest that Nup53 is tightly associated with the NE membrane and the lamina where it interacts with lamin B. We have also shown that Nup53 is capable of physically interacting with a group of nucleoporins including Nup93, Nup155, and Nup205. Consistent with this observation, depletion of Nup53 using small interfering RNAs causes a decrease in the cellular levels of these nucleoporins as well as the spindle checkpoint protein Mad1, likely due to destabilization of Nup53-containing complexes. The cellular depletion of this group of nucleoporins, induced by depleting either Nup53 or Nup93, severely alters nuclear morphology producing phenotypes similar to that previously observed in cells depleted of lamin A and Mad1. On basis of these data, we propose a model in which Nup53 is positioned near the pore membrane and the lamina where it anchors an NPC subcomplex containing Nup93, Nup155, and Nup205.
Aitchison,
Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p.
1995, Pubmed
Aitchison,
Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p.
1995,
Pubmed
Aitchison,
Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport.
1995,
Pubmed
Allen,
Deciphering networks of protein interactions at the nuclear pore complex.
2002,
Pubmed
Belgareh,
Dynamics of nuclear pore distribution in nucleoporin mutant yeast cells.
1997,
Pubmed
Belgareh,
An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells.
2001,
Pubmed
Blobel,
Nuclei from rat liver: isolation method that combines purity with high yield.
1966,
Pubmed
Boehmer,
Depletion of a single nucleoporin, Nup107, prevents the assembly of a subset of nucleoporins into the nuclear pore complex.
2003,
Pubmed
Burke,
Lamins and apoptosis: a two-way street?
2001,
Pubmed
Campbell,
Mitotic checkpoint proteins HsMAD1 and HsMAD2 are associated with nuclear pore complexes in interphase.
2001,
Pubmed
Chaudhary,
Stepwise reassembly of the nuclear envelope at the end of mitosis.
1993,
Pubmed
Chen,
Spindle checkpoint protein Xmad1 recruits Xmad2 to unattached kinetochores.
1998,
Pubmed
,
Xenbase
Cordes,
Intranuclear filaments containing a nuclear pore complex protein.
1993,
Pubmed
,
Xenbase
Cronshaw,
Proteomic analysis of the mammalian nuclear pore complex.
2002,
Pubmed
De Souza,
Partial nuclear pore complex disassembly during closed mitosis in Aspergillus nidulans.
2004,
Pubmed
De Souza,
The SONB(NUP98) nucleoporin interacts with the NIMA kinase in Aspergillus nidulans.
2003,
Pubmed
Elbashir,
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.
2001,
Pubmed
Fahrenkrog,
The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein import.
2000,
Pubmed
Fontoura,
A conserved biogenesis pathway for nucleoporins: proteolytic processing of a 186-kilodalton precursor generates Nup98 and the novel nucleoporin, Nup96.
1999,
Pubmed
Galy,
Caenorhabditis elegans nucleoporins Nup93 and Nup205 determine the limit of nuclear pore complex size exclusion in vivo.
2003,
Pubmed
,
Xenbase
Gilchrist,
Accelerating the rate of disassembly of karyopherin.cargo complexes.
2002,
Pubmed
Grandi,
Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p.
1995,
Pubmed
Grandi,
Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly.
1997,
Pubmed
,
Xenbase
Harborth,
Identification of essential genes in cultured mammalian cells using small interfering RNAs.
2001,
Pubmed
Harel,
Removal of a single pore subcomplex results in vertebrate nuclei devoid of nuclear pores.
2003,
Pubmed
,
Xenbase
Iouk,
The yeast nuclear pore complex functionally interacts with components of the spindle assembly checkpoint.
2002,
Pubmed
Joseph,
The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo.
2004,
Pubmed
Joseph,
SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles.
2002,
Pubmed
Kosova,
Nup192p is a conserved nucleoporin with a preferential location at the inner site of the nuclear membrane.
1999,
Pubmed
Krull,
Nucleoporins as components of the nuclear pore complex core structure and Tpr as the architectural element of the nuclear basket.
2004,
Pubmed
Lew,
The spindle assembly and spindle position checkpoints.
2003,
Pubmed
Loïodice,
The entire Nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis.
2004,
Pubmed
Luo,
The Mad2 spindle checkpoint protein undergoes similar major conformational changes upon binding to either Mad1 or Cdc20.
2002,
Pubmed
Lusk,
Karyopherins in nuclear pore biogenesis: a role for Kap121p in the assembly of Nup53p into nuclear pore complexes.
2002,
Pubmed
Lutzmann,
Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins.
2002,
Pubmed
Macara,
Transport into and out of the nucleus.
2001,
Pubmed
Makhnevych,
Cell cycle regulated transport controlled by alterations in the nuclear pore complex.
2003,
Pubmed
Marelli,
Specific binding of the karyopherin Kap121p to a subunit of the nuclear pore complex containing Nup53p, Nup59p, and Nup170p.
1998,
Pubmed
Marelli,
A link between the synthesis of nucleoporins and the biogenesis of the nuclear envelope.
2001,
Pubmed
Martin-Lluesma,
Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2.
2002,
Pubmed
Mattaj,
Sorting out the nuclear envelope from the endoplasmic reticulum.
2004,
Pubmed
Mattout-Drubezki,
Dynamic interactions of nuclear lamina proteins with chromatin and transcriptional machinery.
2003,
Pubmed
Matunis,
A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex.
1996,
Pubmed
,
Xenbase
Miller,
Identification of a new vertebrate nucleoporin, Nup188, with the use of a novel organelle trap assay.
2000,
Pubmed
,
Xenbase
Nehrbass,
The yeast nucleoporin Nup188p interacts genetically and physically with the core structures of the nuclear pore complex.
1996,
Pubmed
Radu,
Nup155 is a novel nuclear pore complex protein that contains neither repetitive sequence motifs nor reacts with WGA.
1993,
Pubmed
Ribbeck,
The permeability barrier of nuclear pore complexes appears to operate via hydrophobic exclusion.
2002,
Pubmed
Ribbeck,
Kinetic analysis of translocation through nuclear pore complexes.
2001,
Pubmed
Rout,
The yeast nuclear pore complex: composition, architecture, and transport mechanism.
2000,
Pubmed
Rout,
Virtual gating and nuclear transport: the hole picture.
2003,
Pubmed
Ryan,
The nuclear pore complex: a protein machine bridging the nucleus and cytoplasm.
2000,
Pubmed
Salina,
Nup358 integrates nuclear envelope breakdown with kinetochore assembly.
2003,
Pubmed
Schirmer,
Nuclear membrane proteins with potential disease links found by subtractive proteomics.
2003,
Pubmed
Shulga,
Yeast nucleoporins involved in passive nuclear envelope permeability.
2000,
Pubmed
Siniossoglou,
Structure and assembly of the Nup84p complex.
2000,
Pubmed
Siniossoglou,
A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores.
1996,
Pubmed
Smythe,
Incorporation of the nuclear pore basket protein nup153 into nuclear pore structures is dependent upon lamina assembly: evidence from cell-free extracts of Xenopus eggs.
2000,
Pubmed
,
Xenbase
Sukegawa,
A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm.
1993,
Pubmed
Sullivan,
Loss of A-type lamin expression compromises nuclear envelope integrity leading to muscular dystrophy.
1999,
Pubmed
Vasu,
Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export.
2001,
Pubmed
,
Xenbase
Walther,
The conserved Nup107-160 complex is critical for nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
Weis,
Regulating access to the genome: nucleocytoplasmic transport throughout the cell cycle.
2003,
Pubmed
Wozniak,
Primary structure analysis of an integral membrane glycoprotein of the nuclear pore.
1989,
Pubmed
Yang,
Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications.
1998,
Pubmed
Yoshida,
The karyopherin Kap142p/Msn5p mediates nuclear import and nuclear export of different cargo proteins.
2001,
Pubmed
Zabel,
Nic96p is required for nuclear pore formation and functionally interacts with a novel nucleoporin, Nup188p.
1996,
Pubmed
