Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-23687
Anal Biochem 1992 Jun 01;2032:340-7. doi: 10.1016/0003-2697(92)90322-x.
Show Gene links Show Anatomy links

In vitro transcription by RNA polymerase II in extracts of Xenopus oocytes, eggs, and somatic cells.

Toyoda T , Wolffe AP .


???displayArticle.abstract???
We describe procedures for preparing extracts of Xenopus oocytes, eggs, and somatic cells that will accurately transcribe class II genes. A variety of viral and Xenopus promoters direct the accurate initiation of transcription by RNA polymerase II in these extracts. Optimal ionic conditions (100-200 mM KCl, 12 mM MgCl2), template concentration (20-40 micrograms/ml), incubation time (30-60 min), and temperature (25 degrees C) for class II gene transcription are described.

???displayArticle.pubmedLink??? 1416031
???displayArticle.link??? Anal Biochem