Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Sequence analysis of cytoplasmic mRNA-binding proteins of Xenopus oocytes identifies a family of RNA-binding proteins.
Murray MT
,
Schiller DL
,
Franke WW
.
???displayArticle.abstract???
Storage of maternal mRNAs as nontranslated ribonucleoprotein (RNP) complexes is an adaptive strategy in various vertebrate and invertebrate oocytes, for rapid translational recruitment during embryonic development. Previously, we showed that Xenopus laevis oocytes have a soluble cytoplasmic pool of mRNA-binding proteins and particles competent for messenger RNP assembly in vitro. Here we report the isolation of cDNAs for the most abundant messenger RNPs, the 54- and 56-kDa polypeptide (p54/p56) components of the approximately 6S mRNA-binding particle, from an ovarian expression library. The nucleotide sequence of p56 cDNA is almost identical to that recently reported for the putative Xenopus transcription factor FRG Y2. p54 and p56 are highly homologous and are smaller than expected by SDS/PAGE (36 kDa and 37 kDa) due to anomalous electrophoretic mobility. They lack the "RNP consensus motif" but contain four arginine-rich "basic/aromatic islands" that are similar to the RNA-binding domain of bacteriophage mRNA antiterminator proteins and of tat protein of human immunodeficiency virus. The basic/aromatic regions and a second conspicuous 100-amino acid "domain C" of p54 and p56 are conserved in the following DNA-binding proteins: human proteins dpbA, dpbB, and YB-1, rat protein EFIA, and Xenopus protein FRG Y1, all reported to bind to DNA; domain C is homologous to the major Escherichia coli cold-stress-response protein reportedly involved in translational control. Antibodies raised against a peptide of domain C have identified similar proteins in Xenopus somatic cells and in some mammalian cells and tissues. We conclude that p54 and p56 define a family of RNA-binding proteins, at least some of which may be involved in translational regulation.
Ankenbauer,
Identification of a widespread nuclear actin binding protein.
1989, Pubmed,
Xenbase
Ankenbauer,
Identification of a widespread nuclear actin binding protein.
1989,
Pubmed
,
Xenbase
Bandziulis,
RNA-binding proteins as developmental regulators.
1989,
Pubmed
Beyer,
Identification and characterization of the packaging proteins of core 40S hnRNP particles.
1977,
Pubmed
Blobel,
A protein of molecular weight 78,000 bound to the polyadenylate region of eukaryotic messenger RNAs.
1973,
Pubmed
Broeze,
Effects of low temperature on in vivo and in vitro protein synthesis in Escherichia coli and Pseudomonas fluorescens.
1978,
Pubmed
Bürglin,
Cloning of nucleoplasmin from Xenopus laevis oocytes and analysis of its developmental expression.
1987,
Pubmed
,
Xenbase
Chou,
Empirical predictions of protein conformation.
1978,
Pubmed
Cobianchi,
Mammalian heterogeneous nuclear ribonucleoprotein complex protein A1. Large-scale overproduction in Escherichia coli and cooperative binding to single-stranded nucleic acids.
1988,
Pubmed
Crawford,
An RNA-binding protein from Xenopus oocytes is associated with specific message sequences.
1987,
Pubmed
,
Xenbase
Cummings,
Protein kinase activity associated with stored messenger ribonucleoprotein particles of Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Darnbrough,
Identification in Xenopus laevis of a class of oocyte-specific proteins bound to messenger RNA.
1981,
Pubmed
,
Xenbase
Deschamps,
Purification of two thermostable components of messenger ribonucleoprotein particles (mRNPs) from Xenopus laevis oocytes, belonging to a novel class of RNA-binding proteins.
1991,
Pubmed
,
Xenbase
Didier,
Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class II Y box.
1988,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Dworkin,
Mobilization of specific maternal RNA species into polysomes after fertilization in Xenopus laevis.
1985,
Pubmed
,
Xenbase
Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed
Garnier,
Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
1978,
Pubmed
Goldstein,
Major cold shock protein of Escherichia coli.
1990,
Pubmed
Görlach,
Binding of globin mRNA, beta-globin mRNA segments and RNA homopolymers by immobilized protein of polysomal globin messenger ribonucleoprotein.
1989,
Pubmed
Herrmann,
Expression of intermediate filament proteins during development of Xenopus laevis. I. cDNA clones encoding different forms of vimentin.
1989,
Pubmed
,
Xenbase
Jones,
Induction of proteins in response to low temperature in Escherichia coli.
1987,
Pubmed
Kick,
Phosphorylation of a 60 kDa polypeptide from Xenopus oocytes blocks messenger RNA translation.
1987,
Pubmed
,
Xenbase
Kleinschmidt,
Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Koch,
Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules.
1990,
Pubmed
Krohne,
Immunological identification of the karyophilic, histone-binding proteins N1 and N2 in somatic cells and oocytes of diverse amphibia.
1985,
Pubmed
,
Xenbase
Lapeyre,
Nucleolin, the major nucleolar protein of growing eukaryotic cells: an unusual protein structure revealed by the nucleotide sequence.
1987,
Pubmed
Lazinski,
Sequence-specific recognition of RNA hairpins by bacteriophage antiterminators requires a conserved arginine-rich motif.
1989,
Pubmed
Lee,
Temporal and spatial regulation of fibronectin in early Xenopus development.
1984,
Pubmed
,
Xenbase
Legocki,
Multiple immunoreplica Technique: screening for specific proteins with a series of different antibodies using one polyacrylamide gel.
1981,
Pubmed
Leibold,
Cytoplasmic protein binds in vitro to a highly conserved sequence in the 5' untranslated region of ferritin heavy- and light-subunit mRNAs.
1988,
Pubmed
Malter,
Identification of an AUUUA-specific messenger RNA binding protein.
1989,
Pubmed
Milburn,
Cloning and expression of eukaryotic initiation factor 4B cDNA: sequence determination identifies a common RNA recognition motif.
1990,
Pubmed
Moldave,
Eukaryotic protein synthesis.
1985,
Pubmed
Moore,
Site-specific polyadenylation in a cell-free reaction.
1984,
Pubmed
Murray,
Different forms of soluble cytoplasmic mRNA binding proteins and particles in Xenopus laevis oocytes and embryos.
1991,
Pubmed
,
Xenbase
Nevins,
The pathway of eukaryotic mRNA formation.
1983,
Pubmed
Ozer,
Isolation and characterization of a cDNA clone for the CCAAT transcription factor EFIA reveals a novel structural motif.
1990,
Pubmed
,
Xenbase
Richter,
Information relay from gene to protein: the mRNP connection.
1988,
Pubmed
Richter,
A monoclonal antibody to an oocyte-specific poly(A) RNA-binding protein.
1984,
Pubmed
,
Xenbase
Richter,
Reversible inhibition of translation by Xenopus oocyte-specific proteins.
,
Pubmed
,
Xenbase
Rouault,
Binding of a cytosolic protein to the iron-responsive element of human ferritin messenger RNA.
1988,
Pubmed
Sachs,
A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: domain structure and expression.
1986,
Pubmed
Sakura,
Two human genes isolated by a novel method encode DNA-binding proteins containing a common region of homology.
1988,
Pubmed
Shaw,
A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
1986,
Pubmed
Spirin,
"Masked" forms of mRNA.
1966,
Pubmed
Stick,
Changes in the nuclear lamina composition during early development of Xenopus laevis.
1985,
Pubmed
,
Xenbase
Tafuri,
Xenopus Y-box transcription factors: molecular cloning, functional analysis and developmental regulation.
1990,
Pubmed
,
Xenbase
Walden,
Translational repression in eukaryotes: partial purification and characterization of a repressor of ferritin mRNA translation.
1988,
Pubmed
Wistow,
Cold shock and DNA binding.
1990,
Pubmed
Wolffe,
Developmental regulation of two 5S ribosomal RNA genes.
1988,
Pubmed
,
Xenbase
Woodland,
Changes in the polysome content of developing Xenopus laevis embryos.
1974,
Pubmed
,
Xenbase
Woodland,
Utilization of stored mRNA in Xenopus embryos and its replacement by newly synthesized transcripts: histone H1 synthesis using interspecies hybrids.
1979,
Pubmed
,
Xenbase
