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Mol Biol Cell
2005 Mar 01;163:1152-64. doi: 10.1091/mbc.e04-07-0585.
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XGef mediates early CPEB phosphorylation during Xenopus oocyte meiotic maturation.
Martínez SE
,
Yuan L
,
Lacza C
,
Ransom H
,
Mahon GM
,
Whitehead IP
,
Hake LE
.
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Polyadenylation-induced translation is an important regulatory mechanism during metazoan development. During Xenopus oocyte meiotic progression, polyadenylation-induced translation is regulated by CPEB, which is activated by phosphorylation. XGef, a guanine exchange factor, is a CPEB-interacting protein involved in the early steps of progesterone-stimulated oocyte maturation. We find that XGef influences early oocyte maturation by directly influencing CPEB function. XGef and CPEB interact during oogenesis and oocyte maturation and are present in a c-mos messenger ribonucleoprotein (mRNP). Both proteins also interact directly in vitro. XGef overexpression increases the level of CPEB phosphorylated early during oocyte maturation, and this directly correlates with increased Mos protein accumulation and acceleration of meiotic resumption. To exert this effect, XGef must retain guanine exchange activity and the interaction with CPEB. Overexpression of a guanine exchange deficient version of XGef, which interacts with CPEB, does not enhance early CPEB phosphorylation. Overexpression of a version of XGef that has significantly reduced interaction with CPEB, but retains guanine exchange activity, decreases early CPEB phosphorylation and delays oocyte maturation. Injection of XGef antibodies into oocytes blocks progesterone-induced oocyte maturation and early CPEB phosphorylation. These findings indicate that XGef is involved in early CPEB activation and implicate GTPase signaling in this process.
Aghazadeh,
Structure and mutagenesis of the Dbl homology domain.
1998, Pubmed
Aghazadeh,
Structure and mutagenesis of the Dbl homology domain.
1998,
Pubmed
Aghazadeh,
Structural basis for relief of autoinhibition of the Dbl homology domain of proto-oncogene Vav by tyrosine phosphorylation.
2000,
Pubmed
Anderson,
Processing of adenovirus 2-induced proteins.
1973,
Pubmed
Barkoff,
Translational control of cyclin B1 mRNA during meiotic maturation: coordinated repression and cytoplasmic polyadenylation.
2000,
Pubmed
,
Xenbase
Bayaa,
The classical progesterone receptor mediates Xenopus oocyte maturation through a nongenomic mechanism.
2000,
Pubmed
,
Xenbase
Castro,
Involvement of Aurora A kinase during meiosis I-II transition in Xenopus oocytes.
2003,
Pubmed
,
Xenbase
Charlesworth,
Cytoplasmic polyadenylation element (CPE)- and CPE-binding protein (CPEB)-independent mechanisms regulate early class maternal mRNA translational activation in Xenopus oocytes.
2004,
Pubmed
,
Xenbase
Eng,
An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database.
1994,
Pubmed
Ferby,
A novel p34(cdc2)-binding and activating protein that is necessary and sufficient to trigger G(2)/M progression in Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Fisher,
Dissociation of MAP kinase activation and MPF activation in hormone-stimulated maturation of Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Frank-Vaillant,
Progesterone regulates the accumulation and the activation of Eg2 kinase in Xenopus oocytes.
2000,
Pubmed
,
Xenbase
Gross,
The critical role of the MAP kinase pathway in meiosis II in Xenopus oocytes is mediated by p90(Rsk).
2000,
Pubmed
,
Xenbase
Hake,
CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase
Hart,
Direct stimulation of the guanine nucleotide exchange activity of p115 RhoGEF by Galpha13.
1998,
Pubmed
Howard,
The mitogen-activated protein kinase signaling pathway stimulates mos mRNA cytoplasmic polyadenylation during Xenopus oocyte maturation.
1999,
Pubmed
,
Xenbase
Krendel,
Nucleotide exchange factor GEF-H1 mediates cross-talk between microtubules and the actin cytoskeleton.
2002,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Li,
Directional sensing requires G beta gamma-mediated PAK1 and PIX alpha-dependent activation of Cdc42.
2003,
Pubmed
Liu,
NMR structure and mutagenesis of the N-terminal Dbl homology domain of the nucleotide exchange factor Trio.
1998,
Pubmed
Ma,
Biphasic activation of Aurora-A kinase during the meiosis I- meiosis II transition in Xenopus oocytes.
2003,
Pubmed
,
Xenbase
Maton,
Cdc2-cyclin B triggers H3 kinase activation of Aurora-A in Xenopus oocytes.
2003,
Pubmed
,
Xenbase
Mendez,
Phosphorylation of CPEB by Eg2 mediates the recruitment of CPSF into an active cytoplasmic polyadenylation complex.
2000,
Pubmed
,
Xenbase
Mendez,
Differential mRNA translation and meiotic progression require Cdc2-mediated CPEB destruction.
2002,
Pubmed
,
Xenbase
Mendez,
Translational control by CPEB: a means to the end.
2001,
Pubmed
,
Xenbase
Mendez,
Phosphorylation of CPE binding factor by Eg2 regulates translation of c-mos mRNA.
2000,
Pubmed
,
Xenbase
Nebreda,
Regulation of the meiotic cell cycle in oocytes.
2000,
Pubmed
,
Xenbase
Nebreda,
Newly synthesized protein(s) must associate with p34cdc2 to activate MAP kinase and MPF during progesterone-induced maturation of Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Palmer,
The activation of MAP kinase and p34cdc2/cyclin B during the meiotic maturation of Xenopus oocytes.
2000,
Pubmed
,
Xenbase
Paris,
Maturation-specific polyadenylation: in vitro activation by p34cdc2 and phosphorylation of a 58-kD CPE-binding protein.
1991,
Pubmed
,
Xenbase
Peng,
Proteomics: the move to mixtures.
2001,
Pubmed
Reverte,
CPEB degradation during Xenopus oocyte maturation requires a PEST domain and the 26S proteasome.
2001,
Pubmed
,
Xenbase
Reverte,
XGef is a CPEB-interacting protein involved in Xenopus oocyte maturation.
2003,
Pubmed
,
Xenbase
Sagata,
What does Mos do in oocytes and somatic cells?
1997,
Pubmed
,
Xenbase
Schmidt,
Guanine nucleotide exchange factors for Rho GTPases: turning on the switch.
2002,
Pubmed
Sheets,
Polyadenylation of c-mos mRNA as a control point in Xenopus meiotic maturation.
1995,
Pubmed
,
Xenbase
Shevchenko,
Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels.
1996,
Pubmed
Si,
A neuronal isoform of CPEB regulates local protein synthesis and stabilizes synapse-specific long-term facilitation in aplysia.
2003,
Pubmed
,
Xenbase
Stebbins-Boaz,
CPEB controls the cytoplasmic polyadenylation of cyclin, Cdk2 and c-mos mRNAs and is necessary for oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase
Tian,
Identification of XPR-1, a progesterone receptor required for Xenopus oocyte activation.
2000,
Pubmed
,
Xenbase
Tunquist,
Under arrest: cytostatic factor (CSF)-mediated metaphase arrest in vertebrate eggs.
2003,
Pubmed
,
Xenbase
Westwick,
Transforming potential of Dbl family proteins correlates with transcription from the cyclin D1 promoter but not with activation of Jun NH2-terminal kinase, p38/Mpk2, serum response factor, or c-Jun.
1998,
Pubmed
Whitehead,
Expression cloning of lfc, a novel oncogene with structural similarities to guanine nucleotide exchange factors and to the regulatory region of protein kinase C.
1995,
Pubmed
Whitehead,
Dependence of Dbl and Dbs transformation on MEK and NF-kappaB activation.
1999,
Pubmed
Worthylake,
Crystal structure of Rac1 in complex with the guanine nucleotide exchange region of Tiam1.
2000,
Pubmed
Zhu,
Cloning, expression, and characterization of a membrane progestin receptor and evidence it is an intermediary in meiotic maturation of fish oocytes.
2003,
Pubmed
Zhu,
Identification, classification, and partial characterization of genes in humans and other vertebrates homologous to a fish membrane progestin receptor.
2003,
Pubmed
,
Xenbase
de Moor,
The Mos pathway regulates cytoplasmic polyadenylation in Xenopus oocytes.
1997,
Pubmed
,
Xenbase
de Moor,
Cytoplasmic polyadenylation elements mediate masking and unmasking of cyclin B1 mRNA.
1999,
Pubmed
,
Xenbase
de Moor,
Translational control in vertebrate development.
2001,
Pubmed
