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BAP, a rat liver protein that activates transcription through a promoter element with similarity to the USF/MLTF binding site.
Kugler W
,
Kaling M
,
Ross K
,
Wagner U
,
Ryffel GU
.
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The vitellogenin genes of Xenopus are liver-specifically expressed. An in vitro transcription system derived from rat liver nuclei allowed us to define the cis-element BABS (B-activator binding site) in the promoter of the B1 vitellogenin gene. An oligonucleotide encompassing the region from -53 to -44 linked to a TATA box is sufficient for a tenfold increase of the transcriptional activity. Gel retardation assays with nuclear rat liver proteins reveal two DNA-protein complexes: Complex 1 can be competed by the USF/MLTF binding site of the adeno major late promoter whereas complex 2 is a distinct protein we refer to as BAP (B-activator protein). In vitro transcription experiments in the presence of USF/MLTF binding site as competitor show that BAP is an efficient transcription factor. Based on UV cross-linking we estimate that BAP has a molecular weight of 58 kd. Phosphatase treatment reveals that DNA binding of BAP requires phosphorylation. BABS is also present in the hepatitis B virus enhancer suggesting that it might play a role in the tumorigenic potential of the virus.
Ben-Levy,
Cellular factors that interact with the hepatitis B virus enhancer.
1989, Pubmed
Ben-Levy,
Cellular factors that interact with the hepatitis B virus enhancer.
1989,
Pubmed
Blum,
The molecular biology of hepatitis B virus.
1989,
Pubmed
Boxer,
The sarcomeric actin CArG-binding factor is indistinguishable from the c-fos serum response factor.
1989,
Pubmed
,
Xenbase
Carthew,
An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter.
1985,
Pubmed
Carthew,
The major late transcription factor binds to and activates the mouse metallothionein I promoter.
1987,
Pubmed
Cereghini,
A liver-specific factor essential for albumin transcription differs between differentiated and dedifferentiated rat hepatoma cells.
1988,
Pubmed
Chang,
An NF1-related vitellogenin activator element mediates transcription from the estrogen-regulated Xenopus laevis vitellogenin promoter.
1990,
Pubmed
,
Xenbase
Chen,
Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
1985,
Pubmed
Chodosh,
A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor.
1986,
Pubmed
Chodosh,
The adenovirus major late transcription factor activates the rat gamma-fibrinogen promoter.
1987,
Pubmed
Corthésy,
A nuclear factor I-like activity and a liver-specific repressor govern estrogen-regulated in vitro transcription from the Xenopus laevis vitellogenin B1 promoter.
1989,
Pubmed
,
Xenbase
Courtois,
Purified hepatocyte nuclear factor 1 interacts with a family of hepatocyte-specific promoters.
1988,
Pubmed
Döbbeling,
A cell-specific activator in the Xenopus A2 vitellogenin gene: promoter elements functioning with rat liver nuclear extracts.
1988,
Pubmed
,
Xenbase
Evans,
The steroid and thyroid hormone receptor superfamily.
1988,
Pubmed
Frain,
The liver-specific transcription factor LF-B1 contains a highly diverged homeobox DNA binding domain.
1989,
Pubmed
Gorski,
Tissue-specific in vitro transcription from the mouse albumin promoter.
1986,
Pubmed
Hall,
Transcription factor IIIA gene expression in Xenopus oocytes utilizes a transcription factor similar to the major late transcription factor.
1989,
Pubmed
,
Xenbase
Jones,
Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses.
1988,
Pubmed
Kadonaga,
Affinity purification of sequence-specific DNA binding proteins.
1986,
Pubmed
Kaling,
Transcription factors different from the estrogen receptor stimulate in vitro transcription from promoters containing estrogen response elements.
1990,
Pubmed
Klein-Hitpass,
An estrogen-responsive element derived from the 5' flanking region of the Xenopus vitellogenin A2 gene functions in transfected human cells.
1986,
Pubmed
,
Xenbase
Kugler,
Tissue-specificity of liver gene expression: a common liver-specific promoter element.
1988,
Pubmed
,
Xenbase
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lichtsteiner,
A glycosylated liver-specific transcription factor stimulates transcription of the albumin gene.
1989,
Pubmed
Maniatis,
Regulation of inducible and tissue-specific gene expression.
1987,
Pubmed
May,
Estrogen dramatically decreases albumin mRNA levels and albumin synthesis in Xenopus laevis liver.
1982,
Pubmed
,
Xenbase
Mitchell,
Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins.
1989,
Pubmed
Morgan,
Sp1, a CAAT-binding factor, and the adenovirus major late promoter transcription factor interact with functional regions of the gamma-fibrinogen promoter.
1988,
Pubmed
Prywes,
Phosphorylation of serum response factor, a factor that binds to the serum response element of the c-FOS enhancer.
1988,
Pubmed
Ryffel,
Liver cell specific gene transcription in vitro: the promoter elements HP1 and TATA box are necessary and sufficient to generate a liver-specific promoter.
1989,
Pubmed
,
Xenbase
Sawadogo,
Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay.
1985,
Pubmed
Sawadogo,
Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.
1985,
Pubmed
Sawadogo,
Multiple forms of the human gene-specific transcription factor USF. I. Complete purification and identification of USF from HeLa cell nuclei.
1988,
Pubmed
Sawadogo,
Multiple forms of the human gene-specific transcription factor USF. II. DNA binding properties and transcriptional activity of the purified HeLa USF.
1988,
Pubmed
Schorpp,
Hepatocyte-specific promoter element HP1 of the Xenopus albumin gene interacts with transcriptional factors of mammalian hepatocytes.
1988,
Pubmed
,
Xenbase
Scotto,
Positive and negative regulation of the gene for transcription factor IIIA in Xenopus laevis oocytes.
1989,
Pubmed
,
Xenbase
Seiler-Tuyns,
Identification of estrogen-responsive DNA sequences by transient expression experiments in a human breast cancer cell line.
1986,
Pubmed
,
Xenbase
Struhl,
Helix-turn-helix, zinc-finger, and leucine-zipper motifs for eukaryotic transcriptional regulatory proteins.
1989,
Pubmed
Wahli,
Evolution and expression of vitellogenin genes.
1988,
Pubmed
Wahli,
Xenopus vitellogenin genes.
1985,
Pubmed
,
Xenbase
Walker,
Sequence homologies in the region preceding the transcription initiation site of the liver estrogen-responsive vitellogenin and apo-VLDLII genes.
1984,
Pubmed
,
Xenbase
Wingender,
Compilation of transcription regulating proteins.
1988,
Pubmed
Wray,
Silver staining of proteins in polyacrylamide gels.
1981,
Pubmed
Yamamoto,
Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB.
1988,
Pubmed
