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The nuclear envelope is composed of membranes, nuclear pores, and a nuclear lamina. Using a cell-free nuclear assembly extract derived from Xenopus eggs, we have investigated how these three components interact during nuclear assembly. We find that the Xenopus embryonic lamin protein LIII cannot bind directly to chromatin or membranes when each is present alone, but is readily incorporated into nuclei when both of the components are present together in an assembly extract. We find that depleting lamin LIII from an extract does not prevent formation of an envelope consisting of membranes and nuclear pores. However, these lamin-depleted envelopes are extremely fragile and fail to grow beyond a limited extent. This suggests that lamin assembly is not required during the initial steps of nuclear envelope formation, but is required for later growth and for maintaining the structural integrity of the envelope. We also present results showing that lamins may only be incorporated into nuclei after DNA has been encapsulated within an envelope and nuclear transport has been activated. With respect to nuclear function, our results show that the presence of a nuclear lamina is required for DNA synthesis to occur within assembled nuclei.
Aebi,
The nuclear lamina is a meshwork of intermediate-type filaments.
, Pubmed,
Xenbase
Aebi,
The nuclear lamina is a meshwork of intermediate-type filaments.
,
Pubmed
,
Xenbase
Benavente,
Functional role of newly formed pore complexes in postmitotic nuclear reorganization.
1989,
Pubmed
,
Xenbase
Benavente,
Nucleocytoplasmic sorting of macromolecules following mitosis: fate of nuclear constituents after inhibition of pore complex function.
1989,
Pubmed
Benavente,
Cell type-specific expression of nuclear lamina proteins during development of Xenopus laevis.
1985,
Pubmed
,
Xenbase
Benavente,
Change of karyoskeleton during spermatogenesis of Xenopus: expression of lamin LIV, a nuclear lamina protein specific for the male germ line.
1985,
Pubmed
,
Xenbase
Benavente,
Involvement of nuclear lamins in postmitotic reorganization of chromatin as demonstrated by microinjection of lamin antibodies.
1986,
Pubmed
Burke,
On the cell-free association of lamins A and C with metaphase chromosomes.
1990,
Pubmed
Burke,
A cell free system to study reassembly of the nuclear envelope at the end of mitosis.
1986,
Pubmed
Chen,
Annulate lamellae: comparison of antigenic epitopes of annulate lamellae membranes with the nuclear envelope.
1988,
Pubmed
Fields,
A major 62-kD intranuclear matrix polypeptide is a component of metaphase chromosomes.
1988,
Pubmed
Finlay,
Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores.
1987,
Pubmed
,
Xenbase
Fisher,
cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate filament proteins.
1986,
Pubmed
Forbes,
Spontaneous formation of nucleus-like structures around bacteriophage DNA microinjected into Xenopus eggs.
1983,
Pubmed
,
Xenbase
Franke,
Nuclear lamins and cytoplasmic intermediate filament proteins: a growing multigene family.
1987,
Pubmed
Georgatos,
Lamin B constitutes an intermediate filament attachment site at the nuclear envelope.
1987,
Pubmed
Gerace,
Functional organization of the nuclear envelope.
1988,
Pubmed
Gerace,
The nuclear envelope lamina is reversibly depolymerized during mitosis.
1980,
Pubmed
Gruenbaum,
Drosophila nuclear lamin precursor Dm0 is translated from either of two developmentally regulated mRNA species apparently encoded by a single gene.
1988,
Pubmed
Harland,
Regulated replication of DNA microinjected into eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase
Kessel,
The structure and function of annulate lamellae: porous cytoplasmic and intranuclear membranes.
1983,
Pubmed
Krohne,
Cell type-specific differences in protein composition of nuclear pore complex-lamina structures in oocytes and erythrocytes of Xenopus laevis.
1981,
Pubmed
,
Xenbase
Krohne,
The nuclear lamins. A multigene family of proteins in evolution and differentiation.
1986,
Pubmed
,
Xenbase
Lebel,
Lamins A and C appear during retinoic acid-induced differentiation of mouse embryonal carcinoma cells.
1987,
Pubmed
Loewinger,
Mutations in the nuclear lamin proteins resulting in their aberrant assembly in the cytoplasm.
1988,
Pubmed
Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase
Lohka,
Roles of cytosol and cytoplasmic particles in nuclear envelope assembly and sperm pronuclear formation in cell-free preparations from amphibian eggs.
1984,
Pubmed
,
Xenbase
McKeon,
Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins.
,
Pubmed
Miake-Lye,
Maturation-promoting factor induces nuclear envelope breakdown in cycloheximide-arrested embryos of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Miake-Lye,
Induction of early mitotic events in a cell-free system.
1985,
Pubmed
,
Xenbase
Newmeyer,
In vitro transport of a fluorescent nuclear protein and exclusion of non-nuclear proteins.
1986,
Pubmed
,
Xenbase
Newport,
Regulation of the cell cycle during early Xenopus development.
1984,
Pubmed
,
Xenbase
Newport,
Nuclear reconstitution in vitro: stages of assembly around protein-free DNA.
1987,
Pubmed
,
Xenbase
Newport,
Disassembly of the nucleus in mitotic extracts: membrane vesicularization, lamin disassembly, and chromosome condensation are independent processes.
1987,
Pubmed
,
Xenbase
Newport,
The nucleus: structure, function, and dynamics.
1987,
Pubmed
Ohkura,
The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases.
1989,
Pubmed
Ottaviano,
Phosphorylation of the nuclear lamins during interphase and mitosis.
1985,
Pubmed
Padan,
Isolation and characterization of the Drosophila nuclear envelope otefin cDNA.
1990,
Pubmed
Schatten,
Nuclear lamins and peripheral nuclear antigens during fertilization and embryogenesis in mice and sea urchins.
1985,
Pubmed
Senior,
Integral membrane proteins specific to the inner nuclear membrane and associated with the nuclear lamina.
1988,
Pubmed
Sheehan,
Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopus eggs.
1988,
Pubmed
,
Xenbase
Stewart,
Teratocarcinoma stem cells and early mouse embryos contain only a single major lamin polypeptide closely resembling lamin B.
1987,
Pubmed
Stick,
cDNA cloning of the developmentally regulated lamin LIII of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Stick,
Changes in the nuclear lamina composition during early development of Xenopus laevis.
1985,
Pubmed
,
Xenbase
Wilson,
A trypsin-sensitive receptor on membrane vesicles is required for nuclear envelope formation in vitro.
1988,
Pubmed
,
Xenbase
Worman,
A lamin B receptor in the nuclear envelope.
1988,
Pubmed
