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XB-ART-25724
Cell Differ Dev 1990 Aug 01;312:97-104. doi: 10.1016/0922-3371(90)90013-m.
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Synthesis of a 180 kDa protein is a molecular marker of Xenopus oocyte meiotic maturation.

Smith RC .


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Xenopus oocyte maturation can be induced in vitro by a number of agents including hormones, proto-oncogenes and cyclins. Xenopus oocytes matured by a variety of treatments, including progesterone, insulin, testosterone and injection of Xenopus c-mos RNA, Xenopus cyclin B2 RNA and egg extracts, synthesize a predominant 180 kDa protein (p180). p180 synthesis begins during maturation prior to germinal vesicle breakdown (GVBD) but is prominent only in oocytes that have undergone GVBD. p180 synthesis is thus a useful, reliable molecular marker of oocyte maturation. p180 continues to be synthesized in eggs and embryos up to the gastrula stage of embryogenesis, but not in the neurula or tailbud embryo.

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Species referenced: Xenopus laevis
Genes referenced: ins mos