XB-ART-26093
EMBO J
1990 Feb 01;92:573-82. doi: 10.1002/j.1460-2075.1990.tb08145.x.
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Competition between transcription complex assembly and chromatin assembly on replicating DNA.
Almouzni G
,
Méchali M
,
Wolffe AP
.
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We have used a Xenopus egg extract to show that a competition exists between the assembly of transcription complexes and nucleosomes on replicating 5S DNA. This competition results in the establishment of a transcriptionally repressed state for 5S DNA that is dependent on core histones but not on the precise positioning of the cores. The repression is selective, since satellite I DNA is not significantly repressed under these conditions. We demonstrate that the efficiency of chromatin assembly compared with transcription complex assembly is an important variable in determining gene activity.
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Species referenced: Xenopus laevis
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