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EMBO J
1989 Dec 01;812:3781-91. doi: 10.1002/j.1460-2075.1989.tb08555.x.
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Cooperative binding of estrogen receptor to imperfect estrogen-responsive DNA elements correlates with their synergistic hormone-dependent enhancer activity.
Martinez E
,
Wahli W
.
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The Xenopus vitellogenin (vit) gene B1 estrogen-inducible enhancer is formed by two closely adjacent 13 bp imperfect palindromic estrogen-responsive elements (EREs), i.e. ERE-2 and ERE-1, having one and two base substitutions respectively, when compared to the perfect palindromic consensus ERE (GGTCANNNTGACC). Gene transfer experiments indicate that these degenerated elements, on their own, have a low or no regulatory capacity at all, but in vivo act together synergistically to confer high receptor- and hormone-dependent transcription activation to the heterologous HSV thymidine kinase promoter. Thus, the DNA region upstream of the vitB1 gene comprising these two imperfect EREs separated by 7 bp, was called the vitB1 estrogen-responsive unit (vitB1 ERU). Using in vitro protein-DNA interaction techniques, we demonstrate that estrogen receptor dimers bind cooperatively to the imperfect EREs of the vitB1 ERU. Binding of a first receptor dimer to the more conserved ERE-2 increases approximately 4- to 8-fold the binding affinity of the receptor to the adjacent less conserved ERE-1. Thus, we suggest that the observed synergistic estrogen-dependent transcription activation conferred by the pair of hormone-responsive DNA elements of the vit B1 ERU is the result of cooperative binding of two estrogen receptor dimers to these two adjacent imperfect EREs.
Beato,
Gene regulation by steroid hormones.
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Beato,
Gene regulation by steroid hormones.
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Breathnach,
Plasmids for the cloning and expression of full-length double-stranded cDNAs under control of the SV40 early or late gene promoter.
1983,
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Cato,
DNA sequences outside the receptor-binding sites differently modulate the responsiveness of the mouse mammary tumour virus promoter to various steroid hormones.
1988,
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Dynan,
Isolation of transcription factors that discriminate between different promoters recognized by RNA polymerase II.
1983,
Pubmed
Felgner,
Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
1987,
Pubmed
Fried,
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
1981,
Pubmed
Galas,
DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
1978,
Pubmed
Glass,
The thyroid hormone receptor binds with opposite transcriptional effects to a common sequence motif in thyroid hormone and estrogen response elements.
1988,
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Green,
Nuclear receptors enhance our understanding of transcription regulation.
1988,
Pubmed
Green,
Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A.
,
Pubmed
Greene,
Immunochemical studies of estrogen receptors.
1984,
Pubmed
Jantzen,
Cooperativity of glucocorticoid response elements located far upstream of the tyrosine aminotransferase gene.
1987,
Pubmed
Klein-Hitpass,
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,
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Klein-Hitpass,
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,
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Klein-Hitpass,
Specific binding of estrogen receptor to the estrogen response element.
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,
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Klock,
Oestrogen and glucocorticoid responsive elements are closely related but distinct.
,
Pubmed
Kumar,
Functional domains of the human estrogen receptor.
1987,
Pubmed
Kumar,
The estrogen receptor binds tightly to its responsive element as a ligand-induced homodimer.
1988,
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Martinez,
The estrogen-responsive element as an inducible enhancer: DNA sequence requirements and conversion to a glucocorticoid-responsive element.
1987,
Pubmed
,
Xenbase
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
Person,
Inhibition of 40S--Met--tRNAfMet ribosomal initiation complex formation by vaccinia virus.
1980,
Pubmed
Ptashne,
How eukaryotic transcriptional activators work.
1988,
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Raymondjean,
Several distinct "CCAAT" box binding proteins coexist in eukaryotic cells.
1988,
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Rice,
Vaccinia virus induces cellular mRNA degradation.
1983,
Pubmed
Scheidereit,
Molecular model of the interaction between the glucocorticoid receptor and the regulatory elements of inducible genes.
1986,
Pubmed
Schmid,
Glucocorticoid receptor binds cooperatively to adjacent recognition sites.
1989,
Pubmed
Schüle,
Many transcription factors interact synergistically with steroid receptors.
1988,
Pubmed
Seiler-Tuyns,
The human estrogen receptor can regulate exogenous but not endogenous vitellogenin gene promoters in a Xenopus cell line.
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Pubmed
,
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Strähle,
A DNA sequence of 15 base pairs is sufficient to mediate both glucocorticoid and progesterone induction of gene expression.
1987,
Pubmed
Strähle,
Synergistic action of the glucocorticoid receptor with transcription factors.
1988,
Pubmed
Tora,
Cell-specific activity of a GGTCA half-palindromic oestrogen-responsive element in the chicken ovalbumin gene promoter.
1988,
Pubmed
Tsai,
Cooperative binding of steroid hormone receptors contributes to transcriptional synergism at target enhancer elements.
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Tsai,
Molecular interactions of steroid hormone receptor with its enhancer element: evidence for receptor dimer formation.
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Wakeling,
Biology and mode of action of pure antioestrogens.
1988,
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Walker,
Sequence homologies in the region preceding the transcription initiation site of the liver estrogen-responsive vitellogenin and apo-VLDLII genes.
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,
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Weiler,
The Xenopus laevis estrogen receptor: sequence homology with human and avian receptors and identification of multiple estrogen receptor messenger ribonucleic acids.
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,
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Wigler,
Transformation of mammalian cells with genes from procaryotes and eucaryotes.
1979,
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