Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
In vivo photocrosslinking reveals that transcription factor binding to the mammalian ATF recognition sequence is required for E1A-induced transactivation in injected Xenopus laevis oocytes.
Richter JD
.
???displayArticle.abstract???
The adenovirus E1A 13S mRNA product transactivates genes injected into Xenopus laevis oocytes that are normally E1A-inducible in mammalian cells. However, E1A-stimulated transcription, but not basal (uninduced) transcription, was inhibited if oocytes were incubated in the presence of protein synthesis inhibitors. This suggests that a cellular protein(s) is required for E1A-induced transactivation, but that it is dispensable for basal transcription. In order to identify such a protein from Xenopus oocytes that interacts with the adenovirus E3 promoter, gel shift assays, a new in vivo photocrosslinking assay, and immunoselection of biotinylated oligonucleotides were employed. A protein of molecular size 75 kd, which bound to the mammalian ATF recognition sequence in vivo, was found to be essential for E1A-induced transactivation. Although cycloheximide treatment of oocytes inhibited factor binding. E1A exerted no effect on factor binding. These data suggest that E1A modulates the activity of an oocyte transcription factor, either directly or indirectly, but not its ability to bind DNA.
Berk,
Adenovirus promoters and E1A transactivation.
1986, Pubmed
Berk,
Adenovirus promoters and E1A transactivation.
1986,
Pubmed
Borrelli,
Adenovirus-2 E1A products repress enhancer-induced stimulation of transcription.
,
Pubmed
Chodosh,
A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor.
1986,
Pubmed
Ferguson,
Adenovirus E1a gene product expressed at high levels in Escherichia coli is functional.
1984,
Pubmed
,
Xenbase
Ferguson,
E1A 13S and 12S mRNA products made in Escherichia coli both function as nucleus-localized transcription activators but do not directly bind DNA.
1985,
Pubmed
Garcia,
Upstream regulatory regions required to stabilize binding to the TATA sequence in an adenovirus early promoter.
1987,
Pubmed
Hen,
Repression of the immunoglobulin heavy chain enhancer by the adenovirus-2 E1A products.
1985,
Pubmed
Horikoshi,
Transcription factor ATF interacts with the TATA factor to facilitate establishment of a preinitiation complex.
1988,
Pubmed
Hurst,
Identification of factors that interact with the E1A-inducible adenovirus E3 promoter.
1987,
Pubmed
Imperiale,
Adenovirus 5 E2 transcription unit: an E1A-inducible promoter with an essential element that functions independently of position or orientation.
1984,
Pubmed
Imperiale,
An enhancer-like element in the adenovirus E2 promoter contains sequences essential for uninduced and E1A-induced transcription.
1985,
Pubmed
Jones,
Regulation of adenovirus transcription by an E1a gene in microinjected Xenopus laevis oocytes.
1983,
Pubmed
,
Xenbase
Kingston,
Regulation of transcription of the adenovirus EII promoter by EIa gene products: absence of sequence specificity.
1984,
Pubmed
Kovesdi,
E1A transcription induction: enhanced binding of a factor to upstream promoter sequences.
1986,
Pubmed
Lee,
A cellular transcription factor E4F1 interacts with an E1a-inducible enhancer and mediates constitutive enhancer function in vitro.
1987,
Pubmed
Lee,
A cellular protein, activating transcription factor, activates transcription of multiple E1A-inducible adenovirus early promoters.
1987,
Pubmed
Leff,
Transcriptional analysis of the adenovirus-5 EIII promoter: absence of sequence specificity for stimulation by EIa gene products.
1985,
Pubmed
Lin,
Identification and purification of a Saccharomyces cerevisiae protein with the DNA binding specificity of mammalian activating transcription factor.
1989,
Pubmed
Montminy,
Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene.
,
Pubmed
Nevins,
Induction of the synthesis of a 70,000 dalton mammalian heat shock protein by the adenovirus E1A gene product.
1982,
Pubmed
Richter,
Adenovirus E1A requires synthesis of a cellular protein to establish a stable transcription complex in injected Xenopus laevis oocytes.
1987,
Pubmed
,
Xenbase
Richter,
A first exon-encoded domain of E1A sufficient for posttranslational modification, nuclear-localization, and induction of adenovirus E3 promoter expression in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Sassone-Corsi,
Cyclic AMP induction of early adenovirus promoters involves sequences required for E1A trans-activation.
1988,
Pubmed
SivaRaman,
Two promoter-specific host factors interact with adjacent sequences in an EIA-inducible adenovirus promoter.
1987,
Pubmed
SivaRaman,
Identification of a factor in HeLa cells specific for an upstream transcriptional control sequence of an EIA-inducible adenovirus promoter and its relative abundance in infected and uninfected cells.
1986,
Pubmed
Stein,
HeLa cell beta-tubulin gene transcription is stimulated by adenovirus 5 in parallel with viral early genes by an E1a-dependent mechanism.
1984,
Pubmed
Velcich,
Adenovirus E1a proteins repress transcription from the SV40 early promoter.
1985,
Pubmed
Weeks,
E1A control of gene expression is mediated by sequences 5' to the transcriptional starts of the early viral genes.
1983,
Pubmed
Wu,
The E1A 13S product of adenovirus 5 activates transcription of the cellular human HSP70 gene.
1986,
Pubmed
