Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1989 Jun 01;86:1665-70. doi: 10.1002/j.1460-2075.1989.tb03557.x.
Show Gene links
Show Anatomy links
GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs.
Ymer S
,
Schofield PR
,
Draguhn A
,
Werner P
,
Köhler M
,
Seeburg PH
.
Abstract
Cloned cDNAs encoding two new beta subunits of the rat and bovine GABAA receptor have been isolated using a degenerate oligonucleotide probe based on a highly conserved peptide sequence in the second transmembrane domain of GABAA receptor subunits. The beta 2 and beta 3 subunits share approximately 72% sequence identity with the previously characterized beta 1 polypeptide. Northern analysis showed that both beta 2 and beta 3 mRNAs are more abundant in the brain than beta 1 mRNA. All three beta subunit encoding cDNAs were also identified in a library constructed from adrenal medulla RNA. Each beta subunit, when co-expressed in Xenopus oocytes with an alpha subunit, forms functional GABAA receptors. These results, together with the known alpha subunit heterogeneity, suggest that a variety of related but functionally distinct GABAA receptor subtypes are generated by different subunit combinations.
Ballivet,
Electrophysiology of a chick neuronal nicotinic acetylcholine receptor expressed in Xenopus oocytes after cDNA injection.
1990, Pubmed,
Xenbase
Ballivet,
Electrophysiology of a chick neuronal nicotinic acetylcholine receptor expressed in Xenopus oocytes after cDNA injection.
1990,
Pubmed
,
Xenbase
Bormann,
gamma-Aminobutyric acid receptor channels in adrenal chromaffin cells: a patch-clamp study.
1985,
Pubmed
Braestrup,
[3H]Propyl beta-carboline-3-carboxylate as a selective radioligand for the BZ1 benzodiazepine receptor subclass.
1981,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Criado,
Evidence that the acetylcholine binding site is not formed by the sequence alpha 127-143 of the acetylcholine receptor.
1986,
Pubmed
Dascal,
Xenopus oocyte resting potential, muscarinic responses and the role of calcium and guanosine 3',5'-cyclic monophosphate.
1984,
Pubmed
,
Xenbase
Feramisco,
Optimal spatial requirements for the location of basic residues in peptide substrates for the cyclic AMP-dependent protein kinase.
1980,
Pubmed
Fuchs,
Various proteins from rat brain, specifically and irreversibly labeled by [3H]flunitrazepam, are distinct alpha-subunits of the GABA-benzodiazepine receptor complex.
1988,
Pubmed
Grandison,
gamma-Aminobutyric acid receptor function in rat anterior pituitary: evidence for control of prolactin release.
1979,
Pubmed
Grenningloh,
Glycine vs GABA receptors.
1987,
Pubmed
Grenningloh,
The strychnine-binding subunit of the glycine receptor shows homology with nicotinic acetylcholine receptors.
1987,
Pubmed
Häring,
Monoclonal antibodies reveal structural homogeneity of gamma-aminobutyric acid/benzodiazepine receptors in different brain areas.
1985,
Pubmed
Imoto,
Location of a delta-subunit region determining ion transport through the acetylcholine receptor channel.
1987,
Pubmed
,
Xenbase
Kataoka,
Intrinsic GABAergic system of adrenal chromaffin cells.
1984,
Pubmed
Leonard,
Evidence that the M2 membrane-spanning region lines the ion channel pore of the nicotinic receptor.
1989,
Pubmed
,
Xenbase
Levitan,
Structural and functional basis for GABAA receptor heterogeneity.
1988,
Pubmed
,
Xenbase
Mamalaki,
The GABAA/benzodiazepine receptor is a heterotetramer of homologous alpha and beta subunits.
1987,
Pubmed
Möhler,
Benzodiazepine receptor protein identified and visualized in brain tissue by a photoaffinity label.
1980,
Pubmed
Pritchett,
Importance of a novel GABAA receptor subunit for benzodiazepine pharmacology.
1989,
Pubmed
Racagni,
GABA of CNS origin in the rat anterior pituitary inhibits prolactin secretion.
1979,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1978,
Pubmed
Schofield,
Sequence and expression of human GABAA receptor alpha 1 and beta 1 subunits.
1989,
Pubmed
Schofield,
Sequence and functional expression of the GABA A receptor shows a ligand-gated receptor super-family.
1987,
Pubmed
,
Xenbase
Seed,
An LFA-3 cDNA encodes a phospholipid-linked membrane protein homologous to its receptor CD2.
1987,
Pubmed
Setzer,
Size heterogeneity in the 3' end of dihydrofolate reductase messenger RNAs in mouse cells.
1981,
Pubmed
Siegel,
The mRNAs encoding GABAA/benzodiazepine receptor subunits are localized in different cell populations of the bovine cerebellum.
1990,
Pubmed
Sieghart,
Properties of [3H]flunitrazepam binding to different benzodiazepine binding proteins.
1983,
Pubmed
Squires,
Some properties of brain specific benzodiazepine receptors: new evidence for multiple receptors.
1979,
Pubmed
Stephenson,
Understanding the GABAA receptor: a chemically gated ion channel.
1988,
Pubmed
Séquier,
Mapping of brain areas containing RNA homologous to cDNAs encoding the alpha and beta subunits of the rat GABAA gamma-aminobutyrate receptor.
1988,
Pubmed
Tosi,
Multiple polyadenylation sites in a mouse alpha-amylase gene.
1981,
Pubmed
Unnerstall,
Benzodiazepine receptors are coupled to a subpopulation of gamma-aminobutyric acid (GABA) receptors: evidence from a quantitative autoradiographic study.
1981,
Pubmed
Vieira,
Production of single-stranded plasmid DNA.
1988,
Pubmed
Voellmy,
Transcription of a Drosophila heat shock gene is heat-induced in Xenopus oocytes.
1982,
Pubmed
,
Xenbase
von Heijne,
A new method for predicting signal sequence cleavage sites.
1986,
Pubmed
