Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1988 Aug 01;8515:5516-20.
Show Gene links
Show Anatomy links
Transcription complexes that program Xenopus 5S RNA genes are stable in vivo.
Darby MK
,
Andrews MT
,
Brown DD
.
???displayArticle.abstract???
The long-term stability of transcription complexes on 5S RNA genes has been demonstrated in vivo. Complexes on oocyte and somatic-type 5S RNA genes injected into Xenopus laevis oocyte nuclei are stable for at least 4 days. Tissue culture cells and mature erythrocytes have equivalent numbers of somatic 5S RNA genes programmed into transcription complexes, yet the former cell type has a greater than 50-fold higher cellular content of transcription factor IIIA (TFIIIA). Functional transcription complexes on somatic 5S RNA genes in nucleated erythrocytes of Xenopus are stable for weeks, perhaps months, even though a mature erythrocyte has less than two molecules of TFIIIA for each somatic 5S RNA gene. These findings strengthen our proposal that stable transcription complexes are a means of maintaining the differentiated state.
Andrews,
Transient activation of oocyte 5S RNA genes in Xenopus embryos by raising the level of the trans-acting factor TFIIIA.
1987, Pubmed,
Xenbase
Andrews,
Transient activation of oocyte 5S RNA genes in Xenopus embryos by raising the level of the trans-acting factor TFIIIA.
1987,
Pubmed
,
Xenbase
Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978,
Pubmed
,
Xenbase
Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase
Bogenhagen,
Nucleotide sequences in Xenopus 5S DNA required for transcription termination.
1981,
Pubmed
,
Xenbase
Bogenhagen,
Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state.
1982,
Pubmed
,
Xenbase
Brown,
High-fidelity transcription of 5S DNA injected into Xenopus oocytes.
1977,
Pubmed
,
Xenbase
Brown,
The role of stable complexes that repress and activate eucaryotic genes.
1984,
Pubmed
,
Xenbase
Brown,
A positive transcription factor controls the differential expression of two 5S RNA genes.
1985,
Pubmed
,
Xenbase
Cizewski,
A stable transcription complex directs mouse ribosomal RNA synthesis by RNA polymerase I.
1983,
Pubmed
Clarkson,
Sequence organization of a cloned tDNA met fragment from Xenopus laevis.
1978,
Pubmed
,
Xenbase
Cozzarelli,
Purified RNA polymerase III accurately and efficiently terminates transcription of 5S RNA genes.
1983,
Pubmed
,
Xenbase
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Gurdon,
The transcription of 5 S DNA injected into Xenopus oocytes.
1978,
Pubmed
,
Xenbase
Hartley,
Cloning multiple copies of a DNA segment.
1981,
Pubmed
Hentschel,
Template-engaged and free RNA polymerases during Xenopus erythroid cell maturation.
1978,
Pubmed
,
Xenbase
Labarca,
A simple, rapid, and sensitive DNA assay procedure.
1980,
Pubmed
Lassar,
Transcription of class III genes: formation of preinitiation complexes.
1983,
Pubmed
,
Xenbase
Lassar,
Stable transcription complex on a class III gene in a minichromosome.
1985,
Pubmed
,
Xenbase
Mattaj,
An enhancer-like sequence within the Xenopus U2 gene promoter facilitates the formation of stable transcription complexes.
,
Pubmed
,
Xenbase
Parker,
Selective and accurate transcription of the Xenopus laevis 5S RNA genes in isolated chromatin by purified RNA polymerase III.
1977,
Pubmed
,
Xenbase
Pelham,
Related 5S RNA transcription factors in Xenopus oocytes and somatic cells.
1981,
Pubmed
,
Xenbase
Rosenfeld,
Purification of nuclear factor I by DNA recognition site affinity chromatography.
1986,
Pubmed
Ryrie,
The yeast mitochondrial ATPase complex. Subunit composition and evidence for a latent protease contaminant.
1979,
Pubmed
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Sakonju,
The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene.
1981,
Pubmed
,
Xenbase
Schlissel,
The transcriptional regulation of Xenopus 5s RNA genes in chromatin: the roles of active stable transcription complexes and histone H1.
1984,
Pubmed
,
Xenbase
Segall,
Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III.
1980,
Pubmed
Shastry,
Altered levels of a 5 S gene-specific transcription factor (TFIIIA) during oogenesis and embryonic development of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Smith,
Domains of the positive transcription factor specific for the Xenopus 5S RNA gene.
1984,
Pubmed
,
Xenbase
Thomas,
The erythroid cells of anaemic Xenopus laevis. I. Studies on cellular morphology and protein and nucleic acid synthesis during differentiation.
1975,
Pubmed
,
Xenbase
Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase
Wandelt,
Formation of stable preinitiation complexes is a prerequisite for ribosomal DNA transcription in vitro.
1983,
Pubmed
Wolffe,
Differential 5S RNA gene expression in vitro.
1987,
Pubmed
,
Xenbase
Wolffe,
A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting it.
1986,
Pubmed
,
Xenbase
Woll,
Nuclear isolation by a modified method of Hewish and Burgoyne: implications for the study of nuclear enzymology.
1981,
Pubmed