Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The enhancement of ribosomal transcription by the recycling of RNA polymerase I.
Mitchelson K
,
Moss T
.
???displayArticle.abstract???
It has been suggested that the tandemly repeated ribosomal genes of eukaryotes may be subject to a special mechanism of transcriptional enhancement, called Readthrough Enhancement, in which transcription factors are recycled. Recent experiments with the mouse ribosomal genes, although consistent with this possibility, were unable to distinguish between true Readthrough Enhancement and promoter occlusion. To test directly for Readthrough Enhancement, the pre-ribosomal RNA of Xenopus laevis was prematurely terminated within the 18S gene on a circular template. This premature termination was found to reduce the efficiency of pre-ribosomal RNA promotion in cis by 80 to 90%. Since the pre-ribosomal RNA is normally terminated only 213 base pairs upstream of its own initiation site, the results strongly suggest that the recycling of RNA polymerase, or Readthrough Enhancement, is a means by which ribosomal transcription is enhanced in Xenopus laevis.
Adhya,
Promoter occlusion: transcription through a promoter may inhibit its activity.
1982, Pubmed
Adhya,
Promoter occlusion: transcription through a promoter may inhibit its activity.
1982,
Pubmed
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed
Birnboim,
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
1979,
Pubmed
De Winter,
A complex array of sequences enhances ribosomal transcription in Xenopus laevis.
1987,
Pubmed
,
Xenbase
De Winter,
Spacer promoters are essential for efficient enhancement of X. laevis ribosomal transcription.
1986,
Pubmed
,
Xenbase
De Winter,
The ribosomal spacer in Xenopus laevis is transcribed as part of the primary ribosomal RNA.
1986,
Pubmed
,
Xenbase
Gay,
Insertion of transposon Tn7 into the Escherichia coli glmS transcriptional terminator.
1986,
Pubmed
Grummt,
A transcription terminator located upstream of the mouse rDNA initiation site affects rRNA synthesis.
1986,
Pubmed
Henderson,
A transcriptional terminator is a novel element of the promoter of the mouse ribosomal RNA gene.
1986,
Pubmed
Horowitz,
Regulation of transcription from tandem and convergent promoters.
1982,
Pubmed
Labhart,
Enhancer-like properties of the 60/81 bp elements in the ribosomal gene spacer of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Labhart,
Characterization of three sites of RNA 3' end formation in the Xenopus ribosomal gene spacer.
1986,
Pubmed
,
Xenbase
Lillehaug,
Phosphorylation of double-stranded DNAs by T4 polynucleotide kinase.
1976,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
McStay,
A termination site for Xenopus RNA polymerase I also acts as an element of an adjacent promoter.
1986,
Pubmed
,
Xenbase
Moss,
Transcription of cloned Xenopus laevis ribosomal DNA microinjected into Xenopus oocytes, and the identification of an RNA polymerase I promoter.
1982,
Pubmed
,
Xenbase
Moss,
A transcriptional function for the repetitive ribosomal spacer in Xenopus laevis.
,
Pubmed
,
Xenbase
Proudfoot,
Transcriptional interference and termination between duplicated alpha-globin gene constructs suggests a novel mechanism for gene regulation.
,
Pubmed
Reeder,
Spacer regulation of Xenopus ribosomal gene transcription: competition in oocytes.
1983,
Pubmed
,
Xenbase
Wickens,
Expression of a chicken chromosomal ovalbumin gene injected into frog oocyte nuclei.
1980,
Pubmed
,
Xenbase
