Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Dissociation of acetylcholine- and cyclic GMP-induced currents in Xenopus oocytes.
Dascal N
,
Lotan I
,
Lass Y
.
???displayArticle.abstract???
In Xenopus follicular oocytes, activation of muscarinic receptors evokes a slow potassium current (H-response); a similar current is evoked by intracellular injection of cyclic guanosine 3',5'-monophosphate, cGMP (Dascal et al. 1984). We have tested the hypothesis that cGMP may be the second messenger that mediates the opening of K channel by acetylcholine (ACh). ACh elevated the intracellular level of cGMP with a time course similar to that of the development of the muscarinic H-response; maximal increase in cGMP concentration above the control was about 0.2 pmole/oocyte. The amount of injected cGMP that produced a detectable K current ("threshold dose") varied between 0.5 and 3 pmole/oocyte. At low doses of cGMP, the slope of log dose-log response curve was about 2.5, suggesting involvement of a biochemical process with a positive cooperativity of at least 3. Higher doses of cGMP evoked, in addition to the outward current, an irregular, rapidly developing, long-lasting inward current, that never reached amplitudes comparable to those of ACh-evoked Cl currents. The K current elicited by cGMP was insensitive to elevation or depletion of external Ca. It was potentiated by isobutylmethylxanthine (IBMX). ACh strongly inhibited the cGMP-evoked K current when applied at the plateau of the latter. 4-Phorbol 12,13-dibutyrate (PDBu) (1 microM) rapidly and completely inhibited the cGMP response. It is concluded, that most of the results presented in this report contradict the hypothesis that cGMP is the intracellular mediator of ACh-induced changes in membrane conductance in the oocytes.
Baud,
Sodium channels induced by depolarization of the Xenopus laevis oocyte.
1982, Pubmed,
Xenbase
Baud,
Sodium channels induced by depolarization of the Xenopus laevis oocyte.
1982,
Pubmed
,
Xenbase
Beam,
Cyclic nucleotides, protein phosphorylation and synaptic function.
1976,
Pubmed
Cicirelli,
Cyclic AMP levels during the maturation of Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Cooke,
The role of calcium in depolarization-secretion coupling at the motor nerve terminal.
1973,
Pubmed
Dascal,
Acetylcholine and phorbol esters inhibit potassium currents evoked by adenosine and cAMP in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Dascal,
Role of calcium mobilization in mediation of acetylcholine-evoked chloride currents in Xenopus laevis oocytes.
1985,
Pubmed
,
Xenbase
Dascal,
Types of muscarinic response in Xenopus oocytes.
1980,
Pubmed
,
Xenbase
Dascal,
Cyclic GMP mimics the muscarinic response in Xenopus oocytes: identity of ionic mechanisms.
1982,
Pubmed
,
Xenbase
Dascal,
Xenopus oocyte resting potential, muscarinic responses and the role of calcium and guanosine 3',5'-cyclic monophosphate.
1984,
Pubmed
,
Xenbase
Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase
Dascal,
Further characterization of the slow muscarinic responses in Xenopus oocytes.
1987,
Pubmed
,
Xenbase
Dodge,
Co-operative action a calcium ions in transmitter release at the neuromuscular junction.
1967,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Fesenko,
Induction by cyclic GMP of cationic conductance in plasma membrane of retinal rod outer segment.
,
Pubmed
Greengard,
Possible role for cyclic nucleotides and phosphorylated membrane proteins in postsynaptic actions of neurotransmitters.
1976,
Pubmed
Kuo,
Cyclic nucleotide-dependent protein kinases. VI. Isolation and partial purification of a protein kinase activated by guanosine 3',5'-monophosphate.
1970,
Pubmed
Kuo,
Cyclic nucleotide-dependent protein kinases. IX. Partial purification and some properties of guanosine 3',5'-monophosphate-dependent and adenosine 3',5'-monophosphate-dependent protein kinases from various tissues and species of Arthropoda.
1971,
Pubmed
Kupfermann,
Role of cyclic nucleotides in excitable cells.
1980,
Pubmed
Kusano,
Acetylcholine receptors in the oocyte membrane.
,
Pubmed
,
Xenbase
Kusano,
Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane.
1982,
Pubmed
,
Xenbase
Levitan,
Phosphorylation of ion channels.
1985,
Pubmed
Lotan,
Adenosine-induced K+ current in Xenopus oocyte and the role of adenosine 3',5'-monophosphate.
1985,
Pubmed
,
Xenbase
Lotan,
Adenosine-induced slow ionic currents in the Xenopus oocyte.
1982,
Pubmed
,
Xenbase
Miledi,
Chloride current induced by injection of calcium into Xenopus oocytes.
1984,
Pubmed
,
Xenbase
Murad,
Properties and regulation of guanylate cyclase and some proposed functions for cyclic GMP.
1979,
Pubmed
Nadler,
Acetylcholine- and inositol 1,4,5-trisphosphate-induced calcium mobilization in Xenopus laevis oocytes.
1986,
Pubmed
,
Xenbase
Oron,
Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes.
,
Pubmed
,
Xenbase
Reuter,
Calcium channel modulation by neurotransmitters, enzymes and drugs.
,
Pubmed
Schultz,
The importance of calcium ions for the regulation of guanosine 3':5'-cyclic monophosphage levels.
1973,
Pubmed
Snider,
Neurotransmitter receptors mediate cyclic GMP formation by involvement of arachidonic acid and lipoxygenase.
1984,
Pubmed
Stinnakre,
Cyclic adenosine monophosphate, calcium, acetylcholine and the current induced by adenosine in the Xenopus oocyte.
1986,
Pubmed
,
Xenbase
Van Renterghem,
beta-Adrenergic induced K+ current in Xenopus oocytes: role of cAMP, inhibition by muscarinic agents.
1985,
Pubmed
,
Xenbase
Vicentini,
Activation of muscarinic receptors in PC12 cells. Correlation between cytosolic Ca2+ rise and phosphoinositide hydrolysis.
1986,
Pubmed
Zwiller,
Protein kinase C catalyzes phosphorylation of guanylate cyclase in vitro.
1985,
Pubmed
