XB-ART-28045Dev Biol 1987 Jul 28;4162:219-27.
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Immunocytochemical analysis of proenkephalin-derived peptides in the amphibian hypothalamus and optic tectum.
[Met5]-Enkephalin-, [Met5]-enkephalin-Arg6-, [Met5]-enkephalin-Arg6-Phe7-, metorphamide- and BAM 22P-like peptides could be localized in the amphibian brain by immunocytochemistry. However, a [Met5]-enkephalin-Arg6-Gly7-Leu8-like peptide could not be detected in the brain of any anuran species with an antiserum that was capable of detecting this octapeptide in mammalian brain. A synenkephalin-like peptide also could not be detected in the anuran brain with an antiserum that was capable of detecting the antigen in bovine and porcine brain. Although the intensity of proenkephalin-like immunoreactivity depended on the antiserum used, its distribution appeared to be identical with all of the effective antisera. Antisera directed against somatostatin and corticotropin-releasing factor stained perikarya, nerve fibers and terminals in the anuran brain with a distribution that was different from antisera directed against proenkephalin-derived peptides. The distribution of proenkephalin-containing perikarya and nerve fibers in the regions of the anuran brain selected for study showed many similarities to the distribution of proenkephalin-containing perikarya and nerve fibers in the same regions of the amniote brain.
PubMed ID: 3113668
Species referenced: Xenopus laevis
Genes referenced: bcl2l11 crh penk pomc sst.1 was
Article Images: [+] show captions
|Fig. 1. Distribution of proenkephalin-like immunoreactivity in the optic tectum of Rana esculenta. All 4 sections are trom the same colchicine-pretreated frog. All 4 antisera stained perikarya, nerve fibers and terminals with the same distribution. Pear-shaped neurons containing proenkephalin-like immunoreactivity are observed in layer 6. The apical processes of these neurons can be followed as far as the superficial layers of the rectum. Dense bands of proenkephalin immunoreactive fibers are observed in layers 3, 5, 8, and lamina C of the superficial neuropil. Some pigmented cells are observed on the outer surface of the tectum. A is from thc lateral part of the rectum while B-D are from the dorsal part of the tectum. A: Met-ENK antiserum; unintensified. B: Met-ENK-Arg 6 antiserum; unintensified. C: ENK-7 (MEAP-I) antiserum; unintensified. D: metorphamide antiserum; unintensified. A, B. C and D ×75.|
|Fig. 2. Distribution of proenkephalin- and somatostatin-like immunoreactivity in the hypothalamus of Bufo marinus. All 4 sections are from the same toad. All 3 proenkephalin-related antisera stained perikarya, nerve fibers and terminals with the same distribution. Proenkephalin immunoreactive fibers are observed in the periventricular part of the preoptic nucleus and the lateral basal hypothalamus. The SRIF antiserum stained perikarya in the dorsal and ventral part of the preoptic nucleus. A: Met-ENK antiserum; nickel intensified. B: Met-ENK-Arg 6 antiserum; nickel intensified. C: metorphamide antiserum; nickel intensified. D: SRIF antiserum; nickel intensified. A, B, C and D ×75.|