Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-28609
Anal Biochem 1986 Jul 01;1561:257-62. doi: 10.1016/0003-2697(86)90181-8.
Show Gene links Show Anatomy links

Li+ uptake into Xenopus and Cynops oocytes injected with exogenous mRNA, observed by flame emission spectroscopy.

Aoshima H , Iio H , Kobayashi S .


???displayArticle.abstract???
Li+ uptake into Xenopus oocytes was measured by flame emission spectroscopy. Li+ uptake into the oocytes increased proportionally with incubation time and was dependent on either pH or temperature. Maximum uptake of Li+ was observed around pH 7. Li+ uptake into Xenopus oocytes increased by a factor of roughly 7 over the range 4-30 degrees C. When mRNA prepared from electroplax of Electrophorus electricus was injected into Xenopus or Cynops oocytes, Li+ uptake into the injected oocytes increased by the addition of carbamylcholine (Carb), an agonist of the acetylcholine receptor (AChR). This increase of Li+ uptake by Carb was inhibited by d-tubocurarine, an antagonist of nicotinic AChR. Thus, a new method was established for detection of the activity of nicotinic AChR synthesized in oocytes injected with exogenous mRNA.

???displayArticle.pubmedLink??? 3740415
???displayArticle.link??? Anal Biochem