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Translation of mRNA injected into Xenopus oocytes is specifically inhibited by antisense RNA.
Harland R
,
Weintraub H
.
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The bacteriophage SP6 promoter and RNA polymerase were used to synthesize sense and antisense RNAs coding for the enzymes thymidine kinase (TK) and chloramphenicol acetyl transferase (CAT). Injection of antisense CAT RNA into frog oocytes inhibited expression of sense CAT mRNA. Similarly, antisense TK RNA inhibited expression of sense TK mRNA. Antisense RNAs were stable in oocytes and had no detectable effect on either the expression of endogenous proteins or on the expression of nonhomologous RNA transcripts. CAT activity expressed from a plasmid transcribed in the oocytenucleus was also inhibited by antisense RNA injected into the oocytecytoplasm. The data suggest that antisense RNA will be useful in identifying the function of specific mRNA sequences during early development of the frog.
Goldberg,
Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene.
1980, Pubmed
Goldberg,
Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene.
1980,
Pubmed
Gorman,
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
1982,
Pubmed
Green,
Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei.
1983,
Pubmed
,
Xenbase
Gurdon,
Message stability in injected frog oocytes: long life of mammalian alpha and beta globin messages.
1973,
Pubmed
,
Xenbase
Harland,
Transcription of DNA injected into Xenopus oocytes is influenced by template topology.
1983,
Pubmed
,
Xenbase
Izant,
Inhibition of thymidine kinase gene expression by anti-sense RNA: a molecular approach to genetic analysis.
1984,
Pubmed
Konarska,
Recognition of cap structure in splicing in vitro of mRNA precursors.
1984,
Pubmed
Laskey,
The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis.
1980,
Pubmed
Laskey,
Protein synthesis in oocytes of Xenopus laevis is not regulated by the supply of messenger RNA.
1977,
Pubmed
,
Xenbase
McKnight,
The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene.
1980,
Pubmed
Melton,
Injected anti-sense RNAs specifically block messenger RNA translation in vivo.
1985,
Pubmed
,
Xenbase
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Merrill,
Genetic and physical analysis of the chicken tk gene.
1984,
Pubmed
Preiss,
Molecular genetics of Krüppel, a gene required for segmentation of the Drosophila embryo.
,
Pubmed
Rubenstein,
[Nonsense RNA: a tool for specifically inhibiting the expression of a gene in vivo].
1984,
Pubmed
Rusconi,
Transformation of frog embryos with a rabbit beta-globin gene.
1981,
Pubmed
,
Xenbase
