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Proc Natl Acad Sci U S A
1985 Aug 01;8215:4949-53.
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Isolation and functional analysis of a human 70,000-dalton heat shock protein gene segment.
Voellmy R
,
Ahmed A
,
Schiller P
,
Bromley P
,
Rungger D
.
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A human 70-kDa heat shock protein (hsp70) gene segment has been isolated. The segment contains 3.15 kilobase pairs (kbp) of 5' nontranscribed sequence, an RNA leader of 119 bp, and a protein-coding region of 741 bp. The human protein sequence shows a high degree of homology to hsp70 sequences from other species. Expression experiments in Xenopus oocytes and mammalian cells indicate that a region that includes only 105 bp of 5' nontranscribed sequence contains all elements required for the efficient heat-controlled expression of the human gene. Two adjacent identical sequence elements, which are partly homologous to the Drosophila "heat shock consensus" sequence, are located 57 to 76 bp upstream from the capping site. Interestingly, the capping site itself is flanked by inverted repeat sequences.
Amin,
The heat shock consensus sequence is not sufficient for hsp70 gene expression in Drosophila melanogaster.
1985, Pubmed
Amin,
The heat shock consensus sequence is not sufficient for hsp70 gene expression in Drosophila melanogaster.
1985,
Pubmed
Bardwell,
Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous.
1984,
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Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
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Bienz,
Xenopus hsp 70 genes are constitutively expressed in injected oocytes.
1984,
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Casadaban,
Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast.
1983,
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Integration, transcription, and control of a Drosophila heat shock gene in mouse cells.
1981,
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Dudler,
Upstream elements necessary for optimal function of the hsp 70 promoter in transformed flies.
1984,
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Giorgi,
Sendai virus contains overlapping genes expressed from a single mRNA.
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Graham,
A new technique for the assay of infectivity of human adenovirus 5 DNA.
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Holmgren,
Sequence homologies in the 5' regions of four Drosophila heat-shock genes.
1981,
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Ingolia,
Sequence of three copies of the gene for the major Drosophila heat shock induced protein and their flanking regions.
1980,
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Ingolia,
Drosophila gene related to the major heat shock-induced gene is transcribed at normal temperatures and not induced by heat shock.
1982,
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Ingolia,
Saccharomyces cerevisiae contains a complex multigene family related to the major heat shock-inducible gene of Drosophila.
1982,
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Karch,
Extensive regions of homology in front of the two hsp70 heat shock variant genes in Drosophila melanogaster.
1981,
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Kingston,
Regulation of heat shock protein 70 gene expression by c-myc.
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Lawn,
The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA.
1978,
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Lawson,
Expression of heat shock-beta-galactosidase hybrid genes in cultured Drosophila cells.
1984,
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Maxam,
A new method for sequencing DNA.
1977,
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Mellon,
Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system.
1981,
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Mirault,
Regulation of heat-shock genes: a DNA sequence upstream of Drosophila hsp70 genes is essential for their induction in monkey cells.
1982,
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Nevins,
Induction of the synthesis of a 70,000 dalton mammalian heat shock protein by the adenovirus E1A gene product.
1982,
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Parker,
A Drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene.
1984,
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Pelham,
A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene.
1982,
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Probst,
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Reynolds,
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1984,
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1978,
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1982,
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Voellmy,
Transcription of a Drosophila heat shock gene is heat-induced in Xenopus oocytes.
1982,
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Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
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