Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cell surface expression of murine, rat, and human Fc receptors by Xenopus oocytes.
Pure E
,
Luster AD
,
Unkeless JC
.
???displayArticle.abstract???
We report that Xenopus laevis oocytes can efficiently translate and insert heterologous membrane receptors into the oocyteplasma membrane, where they can be detected by the binding of either monoclonal antibodies or ligands. Thus, oocytes injected with mRNA from the mouse J774 macrophage-like cell line, the rat RBL-1 basophilic leukemia, and the U937 promonocyte cell line, bound 2.4G2 Fab, rat IgE, and mouse IgG2a, respectively. The increase in the high avidity Fc gamma R observed after gamma-interferon induction of U937 cells was also observed after injection of mRNA from gamma-interferon-induced U937 cells into oocytes. This suggests either much greater message stability or a greater rate of transcription of Fc gamma Rhi mRNA in the gamma-interferon-induced cells. The assay affords a sensitive method for the detection of rare mRNA species that code for plasma membrane proteins.
Colman,
Export of proteins from oocytes of Xenopus laevis.
1979, Pubmed,
Xenbase
Colman,
Export of proteins from oocytes of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Colman,
The influence of topology and glycosylation on the fate of heterologous secretory proteins made in Xenopus oocytes.
1981,
Pubmed
,
Xenbase
Dickler,
Lymphocyte receptors for immunoglobulin.
1976,
Pubmed
Gundersen,
Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes.
,
Pubmed
,
Xenbase
Guyre,
Recombinant immune interferon increases immunoglobulin G Fc receptors on cultured human mononuclear phagocytes.
1983,
Pubmed
Hoover,
T cells with Fc receptors for IgA: induction of T alpha cells in vivo and in vitro by purified IgA.
1981,
Pubmed
Kulczycki,
The interaction of IgE with rat basophilic leukemia cells. II. Quantitative aspects of the binding reaction.
1974,
Pubmed
Labarca,
MRNA-directed synthesis of catalytically active mouse beta-glucuronidase in Xenopus oocytes.
1977,
Pubmed
,
Xenbase
Lane,
Sequestration and turnover of guinea-pig milk proteins and chicken ovalbumin in Xenopus oocytes.
1979,
Pubmed
,
Xenbase
Lane,
The Xenopus oocyte as a surrogate secretory system. The specificity of protein export.
1980,
Pubmed
,
Xenbase
Liu,
Monoclonal dinitrophenyl-specific murine IgE antibody: preparation, isolation, and characterization.
1980,
Pubmed
Mellman,
Selective iodination and polypeptide composition of pinocytic vesicles.
1980,
Pubmed
Mendez,
Cell-free synthesis of acetylcholine receptor polypeptides.
1980,
Pubmed
Soreq,
Biosynthesis and secretion of catalytically active acetylcholinesterase in Xenopus oocytes microinjected with mRNA from rat brain and from Torpedo electric organ.
1982,
Pubmed
,
Xenbase
Sumikawa,
Active multi-subunit ACh receptor assembled by translation of heterologous mRNA in Xenopus oocytes.
1981,
Pubmed
,
Xenbase
Ullrich,
Rat insulin genes: construction of plasmids containing the coding sequences.
1977,
Pubmed
Unkeless,
Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors.
1979,
Pubmed
Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase
