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Envelope proteins of Semliki Forest virus synthesized in Xenopus oocytes are transported to the cell surface.
Huth A
,
Rapoport TA
,
Kääriäinen L
.
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The mRNA coding for the structural proteins of Semliki Forest virus, the 26S RNA, was injected into Xenopus oocytes. Synthesis of the capsid protein and the three envelope glycoproteins E1, E2 and E2 was observed. The proteins, which are normally incorporated into the plasma membrane of infected cells, are transported to the surface of the oocytes. The transport of the membrane proteins takes place in the presence of tunicamycin. The results show that the proteins foreign to the oocyte reach their destination in the plasma membrane. Consequently, the mRNA contains the information for the transport and proteolytic cleavage of the polypeptides.
Arias,
Sequence analysis of two mutants of Sindbis virus defective in the intracellular transport of their glycoproteins.
1983, Pubmed
Arias,
Sequence analysis of two mutants of Sindbis virus defective in the intracellular transport of their glycoproteins.
1983,
Pubmed
Barnard,
Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes.
1982,
Pubmed
,
Xenbase
Bonatti,
Membrane biogenesis. In vitro cleavage, core glycosylation, and integration into microsomal membranes of sindbis virus glycoproteins.
1979,
Pubmed
Bonner,
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
1974,
Pubmed
Bonner,
Protein migration into nuclei. II. Frog oocyte nuclei accumulate a class of microinjected oocyte nuclear proteins and exclude a class of microinjected oocyte cytoplasmic proteins.
1975,
Pubmed
,
Xenbase
Colman,
The influence of topology and glycosylation on the fate of heterologous secretory proteins made in Xenopus oocytes.
1981,
Pubmed
,
Xenbase
Colman,
Export of proteins from oocytes of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Feldherr,
Mechanism for the selection of nuclear polypeptides in Xenopus oocytes. II. Two-dimensional gel analysis.
1980,
Pubmed
,
Xenbase
Garoff,
The amphiphilic membrane glycoproteins of Semliki Forest virus are attached to the lipid bilayer by their COOH-terminal ends.
1978,
Pubmed
Garoff,
Assembly of the Semliki Forest virus membrane glycoproteins in the membrane of the endoplasmic reticulum in vitro.
1978,
Pubmed
Garoff,
Nucleotide sequence of cdna coding for Semliki Forest virus membrane glycoproteins.
1980,
Pubmed
Gibson,
The nonglycosylated glycoprotein of vesicular stomatitis virus is temperature-sensitive and undergoes intracellular aggregation at elevated temperatures.
1979,
Pubmed
Glanville,
Simultaneous translation of structural and nonstructural proteins from Semliki-forest-virus RNA in two eukaryotic systems in vitro.
1976,
Pubmed
Green,
Passage of viral membrane proteins through the Golgi complex.
1981,
Pubmed
Gurdon,
Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes.
1968,
Pubmed
,
Xenbase
Gurdon,
Nuclear transplantation and the control of gene activity in animal development.
1970,
Pubmed
Hashimoto,
Evidence for a separate signal sequence for the carboxy-terminal envelope glycoprotein E1 of Semliki forest virus.
1981,
Pubmed
Hunt,
Oligosaccharide chains are trimmed during synthesis of the envelope glycoprotein of vesicular stomatitis virus.
1978,
Pubmed
Jones,
Cleavage of a viral envelope precursor during the morphogenesis of Sindbis virus.
1974,
Pubmed
Keränen,
Proteins synthesized by Semliki Forest virus and its 16 temperature-sensitive mutants.
1975,
Pubmed
Kondor-Koch,
Expression of the structural proteins of Semliki Forest virus from cloned cDNA microinjected into the nucleus of baby hamster kidney cells.
1982,
Pubmed
Kääriäinen,
Structure and replication of alpha-viruses.
1978,
Pubmed
Kääriäinen,
Monensin and FCCP inhibit the intracellular transport of alphavirus membrane glycoproteins.
1980,
Pubmed
Lane,
The fate of foreign proteins introduced in Xenopus oocytes.
1981,
Pubmed
,
Xenbase
Lane,
Sequestration and turnover of guinea-pig milk proteins and chicken ovalbumin in Xenopus oocytes.
1979,
Pubmed
,
Xenbase
Lane,
The Xenopus oocyte as a surrogate secretory system. The specificity of protein export.
1980,
Pubmed
,
Xenbase
Lane,
The sequestration, processing and retention of honey-bee promelittin made in amphibian oocytes.
1981,
Pubmed
,
Xenbase
Leavitt,
Impaired intracellular migration and altered solubility of nonglycosylated glycoproteins of vesicular stomatitis virus and Sindbis virus.
1977,
Pubmed
Lebleu,
Translation of mouse interferon mRNA in Xenopus laevis oocytes and in rabbit reticulocyte lysates.
1978,
Pubmed
,
Xenbase
Neville,
Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system.
1971,
Pubmed
Ohlsson,
Synthesis and insertion, both in vivo and in vitro, of rat-liver cytochrome P-450 and epoxide hydratase into Xenopus laevis membranes.
1981,
Pubmed
,
Xenbase
Pesonen,
Incomplete complex oligosaccharides in semliki forest virus envelope proteins arrested within the cell in the presence of monensin.
1982,
Pubmed
Pesonen,
Reversible defect in the glycosylation of the membrane proteins of Semliki Forest virus ts-1 mutant.
1981,
Pubmed
Rapoport,
Intracellular compartmentation and secretion of carp proinsulin synthesized in Xenopus oocytes.
1981,
Pubmed
,
Xenbase
Saraste,
Semliki forest virus mutants with temperature-sensitive transport defect of envelope proteins.
1980,
Pubmed
Saraste,
Reversible transport defects of virus membrane glycoproteins in Sindbis virus mutant infected cells.
1980,
Pubmed
Schlesinger,
Formation of Sindbis virus proteins: identification of a precursor for one of the envelope proteins.
1972,
Pubmed
Schwarz,
Suppression of glycoprotein formation of Semliki Forest, influenza, and avian sarcoma virus by tunicamycin.
1976,
Pubmed
Sefton,
Immediate glycosylation of Sindbis virus membrane proteins.
1977,
Pubmed
Soreq,
Secreted proteins in the medium of microinjected Xenopus oocytes are degraded by oocyte proteases.
1981,
Pubmed
,
Xenbase
Soreq,
Biosynthesis and secretion of catalytically active acetylcholinesterase in Xenopus oocytes microinjected with mRNA from rat brain and from Torpedo electric organ.
1982,
Pubmed
,
Xenbase
Sumikawa,
Active multi-subunit ACh receptor assembled by translation of heterologous mRNA in Xenopus oocytes.
1981,
Pubmed
,
Xenbase
Tkacz,
Tunicamycin inhibition of polyisoprenyl N-acetylglucosaminyl pyrophosphate formation in calf-liver microsomes.
1975,
Pubmed
Väänänen,
The use of red cells with fused Semliki Forest virus envelope proteins in antibody determinations by hemolysis in gel.
1982,
Pubmed
Zehavi-Willner,
Subcellular compartmentation of albumin and globin made in oocytes under the direction of injected messenger RNA.
1977,
Pubmed
,
Xenbase
Ziemiecki,
Formation of the Semliki Forest virus membrane glycoprotein complexes in the infected cell.
1980,
Pubmed
