Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Regular arrangement of nucleosomes on 5S rRNA genes in Xenopus laevis.
Young D
,
Carroll D
.
???displayArticle.abstract???
The chromatin structure of the oocyte-type 5S RNA genes in Xenopus laevis was investigated. Blot hybridization analysis of DNA from micrococcal nuclease digests of erythrocyte nuclei showed that 5S DNA has the same average nucleosome repeat length, 192 +/- 4 base pairs, as two Xenopus satellite DNAs and bulk erythrocyte chromatin. The positions of nuclease-sensitive regions in the 5S DNA repeats of purified DNA and chromatin from erythrocytes were mapped by using an indirect end-labeling technique. Although most of the sites cleaved in purified DNA were also cleaved in chromatin, the patterns of intensities were strikingly different in the two cases. In 5S chromatin, three nuclease-sensitive regions were spaced approximately a nucleosome length apart, suggesting a single, regular arrangement of nucleosomes on most of the 5S DNA repeats. The observed nucleosome locations are discussed with respect to nucleotide sequences known to be important for expression of 5S RNA. Because the preferred locations appear to be reestablished in each repeating unit, despite spacer length heterogeneity, we suggest that the regular chromatin structure reflects the presence of a sequence-specific DNA-binding component on inactive 5S RNA genes.
Baer,
Random location of nucleosomes on genes for 5 S rRNA.
1979, Pubmed
Baer,
Random location of nucleosomes on genes for 5 S rRNA.
1979,
Pubmed
Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978,
Pubmed
,
Xenbase
Bogenhagen,
Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state.
1982,
Pubmed
,
Xenbase
Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase
Brown,
Cloned single repeating units of 5S DNA direct accurate transcription of 5S RNA when injected into Xenopus oocytes.
1978,
Pubmed
,
Xenbase
Brown,
5 S DNAs of Xenopus laevis and Xenopus mulleri: evolution of a gene family.
1973,
Pubmed
,
Xenbase
Brown,
Purification and some characteristics of 5S DNA from Xenopus laevis.
1971,
Pubmed
,
Xenbase
Brown,
High-fidelity transcription of 5S DNA injected into Xenopus oocytes.
1977,
Pubmed
,
Xenbase
Brown,
Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4 s RNA, 5 s RNA and ribosomal RNA.
1968,
Pubmed
,
Xenbase
Bryan,
Nucleosome arrangement on tRNA genes of Xenopus laevis.
1981,
Pubmed
,
Xenbase
Carroll,
Repeating units of Xenopus laevis oocyte-type 5S DNA are heterogeneous in length.
1976,
Pubmed
,
Xenbase
Dingwall,
High sequence specificity of micrococcal nuclease.
1981,
Pubmed
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Fedoroff,
The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. I. The AT-rich spacer.
1978,
Pubmed
,
Xenbase
Fittler,
Subunit structure of alpha-satellite DNA containing chromatin from African green monkey cells.
1979,
Pubmed
Ford,
Different sequences for 5S RNA in kidney cells and ovaries of Xenopus laevis.
1973,
Pubmed
,
Xenbase
Gottesfeld,
Nonrandom alignment of nucleosomes on 5S RNA genes of X. laevis.
1980,
Pubmed
,
Xenbase
Gottesfeld,
Organization of 5S genes in chromatin of Xenopus laevis.
1980,
Pubmed
,
Xenbase
Gurdon,
The transcription of 5 S DNA injected into Xenopus oocytes.
1978,
Pubmed
,
Xenbase
Heidecker,
A versatile primer for DNA sequencing in the M13mp2 cloning system.
1980,
Pubmed
Hewish,
Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.
1973,
Pubmed
Humphries,
Chromatin structure of the 5S ribonucleic acid genes of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Hörz,
Sequence specific cleavage of DNA by micrococcal nuclease.
1981,
Pubmed
Igo-Kemenes,
Chromatin.
1982,
Pubmed
Jacq,
A pseudogene structure in 5S DNA of Xenopus laevis.
1977,
Pubmed
,
Xenbase
Keene,
Micrococcal nuclease as a probe of DNA sequence organization and chromatin structure.
1981,
Pubmed
Korn,
Transcription initiation of Xenopus 5S ribosomal RNA genes in vitro.
1979,
Pubmed
,
Xenbase
Korn,
Transcription of Xenopus 5S ribosomal RNA genes.
1982,
Pubmed
,
Xenbase
Korn,
The reactivation of developmentally inert 5S genes in somatic nuclei injected into Xenopus oocytes.
1981,
Pubmed
,
Xenbase
Kornberg,
The location of nucleosomes in chromatin: specific or statistical.
1981,
Pubmed
Louis,
Chromatin structure of the 5S RNA genes of D. melanogaster.
1980,
Pubmed
Maio,
Subunit structure of chromatin and the organization of eukaryotic highly repetitive DNA: recurrent periodicities and models for the evolutionary origins of repetitive DNA.
1977,
Pubmed
Maniatis,
Nucleotide sequence of the rightward operator of phage lambda.
1975,
Pubmed
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
McGhee,
Nucleosome structure.
1980,
Pubmed
Messing,
A system for shotgun DNA sequencing.
1981,
Pubmed
Messing,
Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.
1977,
Pubmed
Miller,
The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. II. The GC-rich region.
1978,
Pubmed
,
Xenbase
Nedospasov,
Non-random cleavage of SV40 DNA in the compact minichromosome and free in solution by micrococcal nuclease.
1980,
Pubmed
Ng,
Transcription of cloned Xenopus 5S RNA genes by X. laevis RNA polymerase III in reconstituted systems.
1979,
Pubmed
,
Xenbase
Parker,
Selective and accurate transcription of the Xenopus laevis 5S RNA genes in isolated chromatin by purified RNA polymerase III.
1977,
Pubmed
,
Xenbase
Pelham,
Related 5S RNA transcription factors in Xenopus oocytes and somatic cells.
1981,
Pubmed
,
Xenbase
ROBERTS,
Studies on the mechanism of action of micrococcal nuclease. 1. Degradation of thymus deoxyribonucleic acid.
1962,
Pubmed
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Sakonju,
The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene.
1981,
Pubmed
,
Xenbase
Segall,
Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III.
1980,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Thomas,
Variation in chromatin structure in two cell types from the same tissue: a short DNA repeat length in cerebral cortex neurons.
1977,
Pubmed
Wahl,
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
1979,
Pubmed
Wegnez,
Sequence heterogeneity of 5 S RNA in Xenopus laevis.
1972,
Pubmed
,
Xenbase
Weil,
Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates.
1979,
Pubmed
,
Xenbase
Wu,
The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.
1980,
Pubmed
