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Translational stability of native and deadenylylated rabbit globin mRNA injected into HeLa cells.
Huez G
,
Bruck C
,
Cleuter Y
.
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HeLa human cells were injected with a natural mixture of rabbit alpha and beta globin mRNA. They were incubated for 6 hr with [35S]methionine either immediately after injection or 20 hr later. The labeled proteins in the injected cells were analyzed by fluorography of two-dimensional electrophoresis gels. By using this procedure, it was possible to show that, during the first few hours after injection, both alpha and beta globin molecules are synthesized with an alpha to beta ratio approximately equal to 0.6. The rate of synthesis of alpha globin decreased significantly faster than that of beta globin over a 26-hr period after injection of the two mRNAs. It thus seems that two messenger RNAs coding for closely related polypeptides possess a markedly different translational stability. When deadenylylated rabbit globin mRNAs were injected into HeLa cells, no globin synthesis could be detected by the techniques used. We conclude that the translational half-life of mRNAs lacking poly(A) is very short in these cells. It is thus clear that the poly(A) segment is required to ensure stability to globin mRNA in somatic cells as in Xenopus oocytes.
Bonner,
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
1974, Pubmed
Bonner,
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
1974,
Pubmed
Brawerman,
Metabolism of the polyadenylate sequence of nuclear RNA and messenger RNA in mammalian cells.
1975,
Pubmed
Graessmann,
"Early" simian-virus-40-specific RNA contains information for tumor antigen formation and chromatin replication.
1976,
Pubmed
Greenberg,
Isolation of L-cell messenger RNA which lacks poly(adenylate).
1976,
Pubmed
Grässmann,
[The formation of melanin in muscle cells after the direct transfer of RNA from Harding-Passey melanoma cells].
1971,
Pubmed
Huez,
Readenylation of polyadenylate-free globin messenger RNA restores its stability in vivo.
1975,
Pubmed
,
Xenbase
Huez,
Functional stabilisation of HeLa cell histone messenger RNAs injected into Xenopus oocytes by 3'-OH polyadenylation.
1978,
Pubmed
,
Xenbase
Huez,
Translation of rabbit globin mRNA upon injection into fused HeLa cells.
1980,
Pubmed
Huez,
Role of the polyadenylate segment in the translation of globin messenger RNA in Xenopus oocytes.
1974,
Pubmed
,
Xenbase
Lane,
Rabbit haemoglobin synthesis in frog cells: the translation of reticulocyte 9 s RNA in frog oocytes.
1971,
Pubmed
Maniatis,
Translation and stability of human globin mRNA in Xenopus oocytes.
1976,
Pubmed
,
Xenbase
Marbaix,
Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes.
1975,
Pubmed
,
Xenbase
Nokin,
Molecular modifications associated with Aging of globin messenger RNA in vitro.
1976,
Pubmed
O'Farrell,
High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
1977,
Pubmed
Sehgal,
Does 3'-terminal poly(A) stabilize human fibroblast interferon mRNA in oocytes of Xenopus laevis?
1978,
Pubmed
,
Xenbase
Sippel,
Translational properties of rabbit globin mRNA after specific removal of poly(A) with ribonuclease H.
1974,
Pubmed
Stacey,
Microinjection studies of duck globin messenger RNA translation in human and avian cells.
1976,
Pubmed
